Rhizospheric PGPR Strains of Wheat, Barley and Trefoil Grown in Ağrı Province

Year 2021, Volume: 10 Issue: 2, 182 - 190, 31.12.2021

Burak Alaylar Medine Güllüce Mehmet Karadayı Taha Yasin Koç Gökçe Karadayı

https://doi.org/10.46810/tdfd.937841

Cited By: 2

Abstract

The present study aimed to isolate potential plant growth rhizobacteria (PGPR) from various crops in agricultural areas in Ağrı-Turkey and their characterization. For this goal, rhizospheric soil samples of wheat, barley and trefoil were collected from 12 different locations in Ağrı province. Bacteria isolation studies were carried out with these rhizospheric samples. Then, to determine the PGPR properties of each isolate; nitrogen fixation, phosphate dissolution, siderophore, ammonia and HCN production tests were performed. Molecular identification of active isolates determined as suitable for development of biofertilizers, biostimulants and/or bioprotectants was done by PCR and sequencing applications performed with universal 16S rRNA primers. According to the results, 29 potential PGPR isolates were determined and their molecular characterization was done. These isolates were distributed in Arthrobacter, Bacillus, Brevibacterium, Enterobacter, Pantoea, Pseudomonas, Microbacterium and Stenotrophomonas according to their 16S rRNA sequence similarities.

Keywords

PGPR, Nitrogen fixation, Phosphate-solubilization, Siderophore production, 16S rRNA

Supporting Institution

Agri Ibrahim Cecen University Scientific Research Project

Project Number

FEF.18.006-2018

Ağrı İlinde Yetiştirilen Buğday, Arpa ve Yoncanın Rhizosferik PGPR Suşları

Abstract

Mevcut çalışmamızda; Ağrı ili ve çevresinde yer alan tarım alanlarında yetişmekte olan buğday, arpa ve yonca gibi zirai mahsullerden potansiyel bitki büyümesini teşvik eden rizobakterilerin (PGPR) izolasyonu ve karakterizasyonu amaçlanmıştır. Bu amaçla, Ağrı ilinin 12 farklı noktasından buğday, arpa ve yonca rizosferik toprak örnekleri toplanmıştır. Bakteri izolasyonu çalışmaları bu rizosferik örneklerle gerçekleştirilmiştir. Daha sonra her bir izolatın PGPR özelliklerini belirlemeye yönelik; azot bağlama, fosfat çözme, siderofor, amonyak ve HCN üretimi testleri yapılmıştır. Biyogübre, biyostimülan ve/veya biyo-koruyucuların geliştirilmesine uygun olduğu belirlenen aktif izolatların moleküler tanımlaması, evrensel 16S rRNA primerlerinin kullanıldığı PCR ve dizileme uygulamaları ile yapılmıştır. Elde edilen Sonuçlara göre 29 potansiyel PGPR izolatı tanılanmış ve moleküler karakterizasyonu yapılmıştır. Bu izolatlar, 16S rRNA sekans benzerliklerine göre Arthrobacter, Bacillus, Brevibacterium, Enterobacter, Pantoea, Pseudomonas, Microbacterium ve Stenotrophomonas cinsleri içerisinde dağılım göstermiştir.

Keywords

PGPR, Azot fiksasyonu, Fosfat çözünmesi, Siderofor üretimi, 16S rRNA

Project Number

FEF.18.006-2018

References

• [1] Wezel A, Casagrande M, Celette F, Vian JF, Ferrer A, et al. Agroecological practices for sustainable agriculture. A review. Agronomy for Sustainable Development. 2014;34:1–20.
• [2] Alaylar B, Güllüce M, Karadayı G, Karadayı M. Isolation of pgpr strains with phosphate solubilizing activity from Erzurum and their molecular evaluation by using newly designed specific primer for pqqB Gene. International Journal of Scientific and Engineering Research. 2018;9(5);103-106.
• [3] Aeron A, Khare E, Jha CK, Meena VS, Abdel Aziz SM, Islam MT, et al. Revisiting the plant growth‑promoting rhizobacteria: lessons from the past and objectives for the future. Archives of Microbiology. 2020;202:665-676.
• [4] Alaylar B, Güllüce M, Karadayı M. Detection of the nifH gene in nitrogen-fixing bacteria from agricultural areas in Erzurum. Fresenius Environmental Bulletin. 2020;29 (2): 809–814.
• [5] Ibal JC, Jung BK, Park CE, Shin JH. Plant growth-promoting rhizobacteria used in South Korea. Applied Biological Chemistry. 2018; 61(6):709-716.
• [6] Egamberdieva D, Davranov K, Wirth S, Hashec A, Abd_Allah EF. Impact of soil salinity on the plant-growth-promoting and biological control abilities of root associated bacteria. Saudi Journal of Biological Sciences. 2017;24(7):1601-1608.
• [7] Abbas-Zadeh P, Saleh-Rastin N, Asadi-Rahmani H, Khavazi K, Soltani A, Shoary-Nejati AR, et al. Plant growth-promoting activities of fluorescent pseudomonads, isolated from the Iranian soils. Acta Physiologiae Plantarum.2010;32:281-288.
• [8] Rasool L, Asgha M, Jamil A, Rehman SU. Identification of Azospirillum species from wheat rhizosphere. The Journal of Animal & Plant Sciences. 2015;25 (4):1081-1086.
• [9] Shaheen T, Khan AA, Rahman M, Qamar MTU, Rahman M. Estimation of genetic diversity of plant growth promoting rhizobacteria (pgpr) strains found in different areas of Pakistan using RAPD and 16S rRNA analysis. The Journal of Animal & Plant Sciences.2015;25(5):1457-1465.
• [10] Karadayı M, Güllüce M, Doğan S, Güllüce E. Isolation and molecular characterization of bacteria with plant growth-promoting characteristics from magnesite mining fields in Kütahya-Turkey. Journal of Applied Biological Sciences. 2016;10(3): 27-32.
• [11] Rehman B, Hassan TUI, Bano A. Potential of indole-3-acetic acid-producing rhizobacteria to resist Pb toxicity in polluted soil. Soil and Sediment Contamination. 2019;28(1): 101-121.
• [12] Banerjee S, Palit R, Sengupta C, Standing D. Stress induced phosphate solubilization by Arthrobacter sp. and Bacillus sp. isolated from tomato rhizosphere. Australian Journal of Crop Science. 2010;4(6):378-383.
• [13] Kumar K, Amaresan N, Bhagat S, Madhuri K, Srivastava RC. Isolation and characterization of rhizobacteria associated with coastal agricultural ecosystem of rhizosphere soils of cultivated vegetable crops. World Journal of Microbiology and Biotechnology. 2011;27: 1625-1632.
• [14] Pérez-Montano F, Alías-Villegas C, Bellogín RA, Del Cerro P, Espuny MR, Jiménez-Guerrero I, et al. Plant growth promotion in cereal and leguminous agricultural important plants: From microorganism capacities to crop production. Microbiological Research. 2014;169(5-6):325-336.
• [15] Habibi S, Djedidi S, Ohkama-Ohtsu N, Sarhadi WA, Kojima K, Rallos RV, et al. Isolation and screening of indigenous plant growth-promoting rhizobacteria from different rice cultivars in Afghanistan soils. Microbes and Environments. 2019;34(4): 347-355.
• [16] Sharan BS, Nehra V. Plant growth promoting rhizobacteria: A critical review. Life Sciences and Medicine Research. 2011;2011:1-30.
• [17] Chuks Kenneth O. Plant growth promoting rhizobacteria (pgpr): A bioprotectant bioinoculant for sustainable agrobiology. A review. International Journal of Advanced Research in Biological Sciences. 2017;4(5):123-142.
• [18] Oleńska E, Małek W, Wójcik M, Swiecicka I, Thijs S, Vangronsveld J, et al. Beneficial features of plant growth-promoting rhizobacteria for improving plant growth and health in challenging conditions: A methodical review. Science of the Total Environment. 2020;743: 1-21.
• [19] Vessey JK. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil. 2003; 255:571-586.
• [20] Jensen HL. Pro Line Soc. NSW; 1942.
• [21] Pikovskaya RI. Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Mikrobiologiya. 1948;17:362–370.
• [22] Louden BC, Haarmann, Lynne AM. Use of blue agar CAS assay for siderophore detection. Journal of Molecular Biology and Biology Education. 2011;12(1):51-53.
• [23] Marques APGC, Pires C, Moreira H, Rangel AOSS, Castro PML. Assessment of the plant growth promotion abilities of sixbacterial isolates using Zea mays as indicator plant. Soil Biology and Biochemistry. 2010;42:1229–1235.
• [24] Bakker AW, Schippers B. Microbial cyanides production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biology and Biochemistry. 1987;19(4):451–457.
• [25] Harley JP, Prescott LM. Laboratory Exercises in Microbiology. New York; USA. McGraw-Hill Press; 2002.
• [26] Sezen S. Isolation and molecular characterization of microorganisms with usage potential for lignite enrichment technology from Afşin-Elbistan lignite mine [dissertation]. Erzurum: University of Ataturk; 2015.
• [27] Baltaci MO, Genc B, Arslan S, Adiguzel G, Adiguzel A. Isolation and characterization of thermophilic bacteria from geothermal areas in Turkey and preliminary research on biotechnologically important enzyme production. Geomicrobiology Journal. 2017; 34(1):53–62.
• [28] Ferchichi N, Toukabri W, Boularess M, Smaoui A, Mhamdi R, Trabelsi D. Isolation, identification and plant growth promotion ability of endophytic bacteria associated with lupine root nodule grown in Tunisian soil. Archives of Microbiology. 2019;201(3): 1333-1349.
• [29] Orhan F. Alleviation of salt stress by halotolerant and halophilic plant growth-promoting bacteria in wheat (Triticum aestivum). Brazilian Journal of Microbiology. 2016;47 (3):621-627.
• [30] Alaylar B, Güllüce M, Karadayı M, Isaoglu M. Rapid detection of phosphate‑solubilizing bacteria from agricultural areas in Erzurum. Current Microbiology. 2019; 76(7):804-809.
• [31] Alaylar B, Egamberdieva D, Gulluce M, Karadayi M, Arora NK. (2020b). Integration of molecular tools in microbial phosphate solubilization research in agriculture perspective. World Journal of Microbiology and Biotechnology. 2020;36(93):1-12.
• [32] Bhattacharyya PN, Jha DK. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology. 2012; 28:1327-1350.
• [33] Kumar P, Dubey RC, Maheshwari DK. Bacillus strains isolated from rhizosphere showed plant growth promoting and antagonistic activity against phytopathogens. Microbiological Research. 2012;167: 493-499.
• [34] Silini-Chérif H, Silini A, Ghoul M, Yadav S. Isolation and characterization of plant growth promoting traits of a rhizobacteria: Pantoea agglomerans Ima2. Pakistan Journal of Biological Sciences. 2012;15 (6): 267-276.
• [35] Kesaulya H, Baharuddin B, Zakaria B, and Syaiful SA. Isolation and physiological characterization of PGPR from potato plant rhizosphere in medium land of Buru Island. First International Symposium on Food and Agro-biodiversity, ISFA 2014. Semerang: Elsevier; 2014. p. 190-199.
• [36] Tara N, Sharan BS. Plant growth promoting traits shown by bacteria Brevibacterium frigrotolerans SMA23 isolated from Aloe vera rhizosphere. Agricultural Science Digest. 2017;37 (3): 226-231.
• [37] Wang S, Xu Y, Li Z. Nitrogen utilization and transformation of Stenotrophomonas maltophilia W-6 with nitrogen-fixing ability. The Preprint Server for Biology. 2018;1-32.
• [38] Nadiéline CV, Ndiaye MF, Fall S, Krasova T, Le Quéré A, Diouf D. Isolation and characterization of potential phosphate solubilizing bacteria in two regions of Senegal. African Journal of Microbiology Research. 2019;13(28):609-618.