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The Use of Macerated Garlic (Allium Sativum L.) Oil in Preventing the Postoperative Adhesions in Rats

Year 2022, Volume: 11 Issue: 4, 79 - 87, 28.12.2022
https://doi.org/10.46810/tdfd.1184982

Abstract

In the present study, the efficiency of intra-abdominal use of the macerated oil obtained from Taşköprü Garlic (Kastamonu/Türkiye), which is one of the products with geographical indication-from European Union, in preventing the postoperative adhesions. The chemical structure of the macerated-oil, prepared using fresh-garlic at a concentration of 1.2 g/ml in olive oil, was analyzed by spectrophotometric and chromatographic methods. In in vivo experiments, Wistar Albino rats (n=8) were applied cecal abrasion under general anesthesia. Abrasion zone was treated with physiological saline solution in control group (K) and different doses of macerated oil (0.5-1 ml) in study groups-(S1 S2). Macroscopic-adhesion-scoring-(MAS) between 0-4 was performed for all the subjects on postoperative days 3-28. After staining the tissue-samples, which were taken from subjects found to have adhesion, histopathological adhesion scoring (HAS) was performed between 0 3. On day 3, adhesion was detected in all the subjects. The adhesions reached MAS-2 level in the K and were in MAS 1 level in the subjects in S1 S2. On day 28, no adhesion was observed in S1-S2, whereas adhesions in K remained at the level MAS-2. Moreover, HAS were found to be HAS-1 in S1 S2-K on day 3 whereas it was found to be HAS-2 in K subjects, where adhesion formation continued, on day 28. As a result, the garlic-macerated-oil, obtained by soaking fresh garlic in olive oil for a time, is a low cost and effective agent for adhesion prevention, which will be easily produced and applied by clinicians.

Thanks

For their contributions to the laboratory analyses and in vivo process during the present study, the authors would like to thank Havva YAZAR, Omar K. H. ALSHAWI, İpek CEYLAN, and Kıymet NURAL and Salma A. Taher MOHAMED (the postgraduate students in Kastamonu University Institute of Science and Technology), and also Dr. Orçun İŞLER ÖZGÜN (Kastamonu University Ihsangazi Vocational High School Department of Veterinary).

References

  • [1] Pamukçu A. Peritoneal adezyonlardan korunmada gliserinin etkisi. Konya. Selçuk University; 2007.
  • [2] Dubuisson J, Botchorishvili R, Perrette S, Bourdel N, Jardon K, Rabischong B, et al. Incidence of intraabdominal adhesions in a continuous series of 1000 laparoscopic procedures. Am J Obstet Gynecol 2010;203:111.e1-3.
  • [3] Şahbaz A, Işık H, Aynıoğlu Ö, Güngördük K, Gün BD. Effect of intraabdominal administration of Allium Sativum (garlic) oil on postoperative peritoneal adhesion. Eur J Obstet Gyn R B. 2014; 177:44–47.
  • [4] Topal U, Göret NE, Göret CC, Özkan ÖF. The effect of Allium sativum in experimental peritoneal adhesion model in rats. Acta Cir Bras. 2019;34(10);e201901002.
  • [5] Tang J, Xiang Z, Bernards MT, Chen S. Peritoneal adhesions: Occurrence, prevention and experimental models. Acta Biomat. 2020:116;84-104.
  • [6] Blauer KL, Collins RL. The effect of intraperitoneal progesteron on postoperative adhesion formation in rabbit. Fertil Steril. 1988;49:144–8.
  • [7] Ali SN, Al Badawi MH, Galhom RA, Badr FM. Macroscopic and microscopic study of Allium Sativum (garlic) oil effects on post-operative intra-abdominal adhesion in diabetic and non diabetic male rats. J Cell Sci Ther. 2016;7(2):240-6
  • [8] Tabibian N, Swehli E, Boyd A, Umbreen A, Tabibian JH. Abdominal adhesions: A practical review of an often overlooked entity. Annals of Medicine and Surgery. 2017;15: 9-13.
  • [10] Kıvçak B. Sarımsağın ilaç endüstrisinde değerlendirilmesi. Taşköprü Sarımsak Paneli, Kastamonu: North Anatolian Development Agency (Document Number 2012-RP-29/31); 2012. p. 59-67.
  • [11] Koyuncu M. Sarımsağın Tarihçesi, Kullanım Alanları, Sarımsağın Faydaları, Taşköprü Sarımsak Paneli, Kastamonu: North Anatolian Development Agency (Document Number 2012-RP-29/31); 2012. p. 11-20.
  • [12] Ferioli F, Giambanelli E, D'Alessandro V, D'Antuono LF. Comparison of two extraction methods (high pressure extraction vs. maceration) for the total and relative amount of hydrophilic and lipophilic organosulfur compounds in garlic cloves and stems. An application to the Italian ecotype “Aglio Rosso di Sulmona” (Sulmona Red Garlic). Food Chem. 2020;312:126086 (9 pages).
  • [13] Kızılaslan N, Tokatlı K. Sarımsağın İnsan Sağlığı Üzerine Etkileri (Effects of garlic on human health). J Togu Heal Sci 2021;1(2):62 71.
  • [14] Staba EJ, Lash L, Staba JE. A commentary on the effects of garlic extraction and formulation on product composition. J Nutr. 2001;131(3):1118‑19.
  • [15] Alshawi OKH. İnsan kolorektal kanser hücrelerine farklı sarımsak (Allium Sativum L.) ekstraktlarının antikarsinojenik etkisinin araştırılması (Investigation of the anticarcinogenic effects of different garlic (Allium Sativum L.) extracts on human colorectal cancer cells). Kastamonu. Kastamonu University; 2021.
  • [16] Turkish Patent and Trademark Institution [Internet]. Taşköprü Sarımsağı; 2010 [cited 2022 May 01]. Available from https://ci.turkpatent.gov.tr/cografi-isaretler/detay/38009
  • [17] Turkish Ministry of Foreign Affairs [Internet]. Taşköprü Garlic and Bayramiç White Registered as Geographical Indications in the European Union; 2021 [cited 2022 May 01]. Available from https://www.ab.gov.tr/taskopru-sarimsagi-ve-bayramic-beyazi-avrupa-birliginde-cografi-isaret-olarak-tescil-edildi_52527.html
  • [18] Srivastava N, Singh A, Kumari P, Nishad JH, Gautam VS, Yadav M, et al. Advances in extraction technologies: isolation and purification of bioactive compounds from biological materials. In: Sinha R, Häder DP, editors. Natural Bioactive Compounds. Natural Bioactive Compounds. India: Academic Press; 2021. p.409–433.
  • [19] Armutçu F, Namuslu M, Yüksel R, Kaya M. Zeytinyağı ve sağlık: biyoaktif bileşenleri, antioksidan özellikleri ve klinik etkileri. Konuralp Tıp Dergisi. 2013; 5(1):60-68.
  • [20] Institutional Animal Care and Use Committee (IACUC) [Internet]. Use of Tribromoethanol (Avertin); 2019 [cited 2020 Apr 04]. Available from https://iacuc.wsu.edu/documents/2016/06/32_avertin_policy_final.pdf/
  • [21] Diehl KH, Hull R, Morton D, Pfister R, Rabemampianina Y, Smith D, et al. A good practice guide to the administration of substances and removal of blood, including routes and volumes. J Appl Toxicol. 2001;21:15–23.
  • [22] Emin N, Koç A, Durkut S, Elçin AE, Elçin YM. Engineering of rat articular cartilage on porous sponges: Effects of TGF-β 1 and microgravity bioreactor culture. Artif Cell Blood Sub. 2008;36 (2):123-137.
  • [23] Yılmaz HG, Taçyıldız IH, Keles C, Gedik E, Kılınç N. Micronized purified flavonoid fraction may prevent formation of intraperitoneal adhesions in rats. Fertil Steril. 2005;84(2):1083-8.
  • [24] Gündüz T. İnstrumental analiz. Ankara University Science Faculty Publishing; 1988.
  • [25] Fellner M, Kneifel W, Gregorits D, Leonhardt W. Identification and antibiotic sensitivity of microbial contaminants from callus cultures of garlic Allium sativum L. and Allium longicuspis Regei. Plant Sci. 1996;113(2):193-201.
  • [26] Ham JS, Lee SG, Kim MK, Oh MH, Jeong SG, Kim DH, et al. Inhibitory activity of garlic fermented by Pediococcus pentosaceus KACC 91419 against antibiotic-resistant pathogens. Asian Austral J Anim. 2010; 23(9):1236-1243.

Sıçanlarda Postoperatif Adezyonların Önlenmesinde Masere Sarımsak (Allium Sativum L.) Yağı Kullanımı

Year 2022, Volume: 11 Issue: 4, 79 - 87, 28.12.2022
https://doi.org/10.46810/tdfd.1184982

Abstract

Sunulan çalışmada, Avrupa Birliği coğrafi işaretli ürünlerden olan “Taşköprü Sarımsağı”ndan (Kastamonu/Türkiye) maserasyonla elde edilen yağın, postoperatif adezyonları önlemede intraabdominal kullanımının etkinliği araştırıldı. Zeytinyağında 1,2 g/ml konsantrasyonda taze sarımsak kullanılarak hazırlanan maserat yağın kimyasal yapısı, spektrofotometrik ve kromatografik yöntemlerle analize edildi. İn vivo deneylerde, Wistar Albino sıçanlara (n=8) genel anestezi altında sekal abrazyon uygulandı. Takiben, abrazyon bölgesine kontrol grubunda-(K) serum fizyolojik, çalışma gruplarında-(S1 S2) ise farklı dozlarda maserat yağı (0,5 ml-1 ml) tatbik edildi. Adezyon oluşumu, postoperatif 3.-28. günlerde ötenazi sonrasında, 0-4 arasında makroskobik adezyon skorlaması (MAS) ile adezyon bölgelerinden alınan doku örneklerinde hematoksilen-eosin, safranin-O ve alsiyan mavisi boyamaları yapıldıktan sonra, 0-3 arasında histopatolojik adezyon skorlaması (HAS) ile değerlendirildi. Üçüncü günde deneklerin tümünde adezyon tespit edildi. Kontrol grubundaki adezyonların MAS-2 seviyesine çıktığı, çalışma grubu deneklerinde ise sadece MAS 1 seviyesinde adezyon oluştuğu görüldü. Yirmi sekizinci günde S1 ve S2 deneklerinde adezyon görülmezken, K deneklerinde adezyonun MAS-2 seviyesinde devam ettiği belirlendi. Ayrıca, oluşan adezyonların histopatolojik adezyon skorlaması, 3.-günde S1, S2 ve K deneklerinde HAS-1, 28.-gün için adezyon oluşumu devam eden K deneklerinde HAS-2 olarak belirlendi. Sonuç olarak, taze sarımsağın bir süre zeytinyağında bekletilmesiyle elde edilen sarımsak maserat yağının, klinisyenler tarafından kolaylıkla üretilip uygulanabilecek, düşük maliyetli ve etkili bir yapışma önleyici ajan olduğu kanaatine varıldı.

References

  • [1] Pamukçu A. Peritoneal adezyonlardan korunmada gliserinin etkisi. Konya. Selçuk University; 2007.
  • [2] Dubuisson J, Botchorishvili R, Perrette S, Bourdel N, Jardon K, Rabischong B, et al. Incidence of intraabdominal adhesions in a continuous series of 1000 laparoscopic procedures. Am J Obstet Gynecol 2010;203:111.e1-3.
  • [3] Şahbaz A, Işık H, Aynıoğlu Ö, Güngördük K, Gün BD. Effect of intraabdominal administration of Allium Sativum (garlic) oil on postoperative peritoneal adhesion. Eur J Obstet Gyn R B. 2014; 177:44–47.
  • [4] Topal U, Göret NE, Göret CC, Özkan ÖF. The effect of Allium sativum in experimental peritoneal adhesion model in rats. Acta Cir Bras. 2019;34(10);e201901002.
  • [5] Tang J, Xiang Z, Bernards MT, Chen S. Peritoneal adhesions: Occurrence, prevention and experimental models. Acta Biomat. 2020:116;84-104.
  • [6] Blauer KL, Collins RL. The effect of intraperitoneal progesteron on postoperative adhesion formation in rabbit. Fertil Steril. 1988;49:144–8.
  • [7] Ali SN, Al Badawi MH, Galhom RA, Badr FM. Macroscopic and microscopic study of Allium Sativum (garlic) oil effects on post-operative intra-abdominal adhesion in diabetic and non diabetic male rats. J Cell Sci Ther. 2016;7(2):240-6
  • [8] Tabibian N, Swehli E, Boyd A, Umbreen A, Tabibian JH. Abdominal adhesions: A practical review of an often overlooked entity. Annals of Medicine and Surgery. 2017;15: 9-13.
  • [10] Kıvçak B. Sarımsağın ilaç endüstrisinde değerlendirilmesi. Taşköprü Sarımsak Paneli, Kastamonu: North Anatolian Development Agency (Document Number 2012-RP-29/31); 2012. p. 59-67.
  • [11] Koyuncu M. Sarımsağın Tarihçesi, Kullanım Alanları, Sarımsağın Faydaları, Taşköprü Sarımsak Paneli, Kastamonu: North Anatolian Development Agency (Document Number 2012-RP-29/31); 2012. p. 11-20.
  • [12] Ferioli F, Giambanelli E, D'Alessandro V, D'Antuono LF. Comparison of two extraction methods (high pressure extraction vs. maceration) for the total and relative amount of hydrophilic and lipophilic organosulfur compounds in garlic cloves and stems. An application to the Italian ecotype “Aglio Rosso di Sulmona” (Sulmona Red Garlic). Food Chem. 2020;312:126086 (9 pages).
  • [13] Kızılaslan N, Tokatlı K. Sarımsağın İnsan Sağlığı Üzerine Etkileri (Effects of garlic on human health). J Togu Heal Sci 2021;1(2):62 71.
  • [14] Staba EJ, Lash L, Staba JE. A commentary on the effects of garlic extraction and formulation on product composition. J Nutr. 2001;131(3):1118‑19.
  • [15] Alshawi OKH. İnsan kolorektal kanser hücrelerine farklı sarımsak (Allium Sativum L.) ekstraktlarının antikarsinojenik etkisinin araştırılması (Investigation of the anticarcinogenic effects of different garlic (Allium Sativum L.) extracts on human colorectal cancer cells). Kastamonu. Kastamonu University; 2021.
  • [16] Turkish Patent and Trademark Institution [Internet]. Taşköprü Sarımsağı; 2010 [cited 2022 May 01]. Available from https://ci.turkpatent.gov.tr/cografi-isaretler/detay/38009
  • [17] Turkish Ministry of Foreign Affairs [Internet]. Taşköprü Garlic and Bayramiç White Registered as Geographical Indications in the European Union; 2021 [cited 2022 May 01]. Available from https://www.ab.gov.tr/taskopru-sarimsagi-ve-bayramic-beyazi-avrupa-birliginde-cografi-isaret-olarak-tescil-edildi_52527.html
  • [18] Srivastava N, Singh A, Kumari P, Nishad JH, Gautam VS, Yadav M, et al. Advances in extraction technologies: isolation and purification of bioactive compounds from biological materials. In: Sinha R, Häder DP, editors. Natural Bioactive Compounds. Natural Bioactive Compounds. India: Academic Press; 2021. p.409–433.
  • [19] Armutçu F, Namuslu M, Yüksel R, Kaya M. Zeytinyağı ve sağlık: biyoaktif bileşenleri, antioksidan özellikleri ve klinik etkileri. Konuralp Tıp Dergisi. 2013; 5(1):60-68.
  • [20] Institutional Animal Care and Use Committee (IACUC) [Internet]. Use of Tribromoethanol (Avertin); 2019 [cited 2020 Apr 04]. Available from https://iacuc.wsu.edu/documents/2016/06/32_avertin_policy_final.pdf/
  • [21] Diehl KH, Hull R, Morton D, Pfister R, Rabemampianina Y, Smith D, et al. A good practice guide to the administration of substances and removal of blood, including routes and volumes. J Appl Toxicol. 2001;21:15–23.
  • [22] Emin N, Koç A, Durkut S, Elçin AE, Elçin YM. Engineering of rat articular cartilage on porous sponges: Effects of TGF-β 1 and microgravity bioreactor culture. Artif Cell Blood Sub. 2008;36 (2):123-137.
  • [23] Yılmaz HG, Taçyıldız IH, Keles C, Gedik E, Kılınç N. Micronized purified flavonoid fraction may prevent formation of intraperitoneal adhesions in rats. Fertil Steril. 2005;84(2):1083-8.
  • [24] Gündüz T. İnstrumental analiz. Ankara University Science Faculty Publishing; 1988.
  • [25] Fellner M, Kneifel W, Gregorits D, Leonhardt W. Identification and antibiotic sensitivity of microbial contaminants from callus cultures of garlic Allium sativum L. and Allium longicuspis Regei. Plant Sci. 1996;113(2):193-201.
  • [26] Ham JS, Lee SG, Kim MK, Oh MH, Jeong SG, Kim DH, et al. Inhibitory activity of garlic fermented by Pediococcus pentosaceus KACC 91419 against antibiotic-resistant pathogens. Asian Austral J Anim. 2010; 23(9):1236-1243.
There are 25 citations in total.

Details

Primary Language English
Subjects Health Care Administration, Veterinary Surgery
Journal Section Articles
Authors

G. Ülke Çalışkan 0000-0002-4542-315X

Nuray Emin 0000-0002-0859-2536

Publication Date December 28, 2022
Published in Issue Year 2022 Volume: 11 Issue: 4

Cite

APA Çalışkan, G. Ü., & Emin, N. (2022). The Use of Macerated Garlic (Allium Sativum L.) Oil in Preventing the Postoperative Adhesions in Rats. Türk Doğa Ve Fen Dergisi, 11(4), 79-87. https://doi.org/10.46810/tdfd.1184982
AMA Çalışkan GÜ, Emin N. The Use of Macerated Garlic (Allium Sativum L.) Oil in Preventing the Postoperative Adhesions in Rats. TJNS. December 2022;11(4):79-87. doi:10.46810/tdfd.1184982
Chicago Çalışkan, G. Ülke, and Nuray Emin. “The Use of Macerated Garlic (Allium Sativum L.) Oil in Preventing the Postoperative Adhesions in Rats”. Türk Doğa Ve Fen Dergisi 11, no. 4 (December 2022): 79-87. https://doi.org/10.46810/tdfd.1184982.
EndNote Çalışkan GÜ, Emin N (December 1, 2022) The Use of Macerated Garlic (Allium Sativum L.) Oil in Preventing the Postoperative Adhesions in Rats. Türk Doğa ve Fen Dergisi 11 4 79–87.
IEEE G. Ü. Çalışkan and N. Emin, “The Use of Macerated Garlic (Allium Sativum L.) Oil in Preventing the Postoperative Adhesions in Rats”, TJNS, vol. 11, no. 4, pp. 79–87, 2022, doi: 10.46810/tdfd.1184982.
ISNAD Çalışkan, G. Ülke - Emin, Nuray. “The Use of Macerated Garlic (Allium Sativum L.) Oil in Preventing the Postoperative Adhesions in Rats”. Türk Doğa ve Fen Dergisi 11/4 (December 2022), 79-87. https://doi.org/10.46810/tdfd.1184982.
JAMA Çalışkan GÜ, Emin N. The Use of Macerated Garlic (Allium Sativum L.) Oil in Preventing the Postoperative Adhesions in Rats. TJNS. 2022;11:79–87.
MLA Çalışkan, G. Ülke and Nuray Emin. “The Use of Macerated Garlic (Allium Sativum L.) Oil in Preventing the Postoperative Adhesions in Rats”. Türk Doğa Ve Fen Dergisi, vol. 11, no. 4, 2022, pp. 79-87, doi:10.46810/tdfd.1184982.
Vancouver Çalışkan GÜ, Emin N. The Use of Macerated Garlic (Allium Sativum L.) Oil in Preventing the Postoperative Adhesions in Rats. TJNS. 2022;11(4):79-87.

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