Farklı Dozlarda Asetilsalisilik Asit Uygulamasının Sıçanlarda Davranış Bozuklukları Üzerindeki Etkilerinin Araştırılması

Year 2024, Volume: 13 Issue: 4, 162 - 172, 30.12.2024

Hasan Şimşek , Özge Kandemir , Nurhan Akaras

https://doi.org/10.46810/tdfd.1563122

Abstract

Aspirin, dünya genelinde en yaygın kullanılan non-steroid anti-inflamatuvar ilaçlardan biridir. Norodejeneratif hastalıklar merkezi sinir sistemini olumsuz etkileyerek bilişsel fonksiyonların gerilemesine neden olur. Aspirin farklı dozlarda farklı farmakolojik aktiviteye sahiptir. Bu nedenle bu çalışmadaki amaç, farklı dozlarda uygulanan aspirin etken maddesi asetilsalisilik asitin (ASA) bilişsel fonksiyonlarda rol oynayan parametreler üzerindeki etkilerinin moleküler ve histolojik yöntemlerle ve davranış testleri ile belirlenmesidir. Bu amaç doğrultusunda 28 Wistar rat 4 gruba ayrılmıştır. Kontrol, ASA-düşük doz (1mg/kg), ASA-orta doz (10mg/kg) ve ASA-yüksek doz (100mg/kg). ASA tek doz intragastrik olarak uygulandı ve 3 saat sonrasında açık alan testi uygulandı. Ardından hipokampüs dokuları alınarak antioksidan kapasite, inflamasyon, apoptozis ve hafızada rol oynayan parametrelerin analizi ile hipokampüs doku yapısı incelendi. ASA özellikle orta dozda antioksidan kapasiteyi arttırıp, inflamasyon ve apoptotik hasarı kısmı olarak azaltmıştır. Yüksek dozda ise tersi etki göstererek hasar düzeylerini arttırmıştır. Benzer etkiler histolojik incelemede de tespit edilmiş olup düşük ve orta dozda yapısal bozulmalar yok iken yüksek dozda yapısal bozukluklar görülmüştür. Açık alan testi bulgularında gruplar arası fark yok iken merkezde geçirilen süre, kat edilen mesafe ve hız ASA orta doz uygulanan grupta kısmen daha yüksektir. Sonuç olarak, ASA düşük ve orta düzeyde bilişsel fonksiyonların iyileşmesine katkı sağlayabilir ancak yüksek dozda bilişsel fonksiyonların bozulmasına neden olabilir.

Keywords

Apoptozis, Aspirin, Davranışsal bozukluklar, İnflamasyon, NSAID

Investigation of the Effects of Acetylsalicylic Acid Administration at Different Doses on Behavioral Disorders in Rats

Abstract

Aspirin is one of the most widely used non-steroidal anti-inflammatory drugs worldwide. Neurodegenerative diseases adversely affect the central nervous system, leading to cognitive decline. Aspirin has different pharmacological activities at different doses. Therefore, this study aimed to determine the effects of acetylsalicylic acid (ASA), the active ingredient of aspirin, administered at different doses on the parameters that play a role in cognitive function using molecular and histological methods and behavioral tests. For this purpose, 28 Wistar rats were divided into 4 groups. Control, ASA-low dose (1mg/kg), ASA-moderate dose (10mg/kg) and ASA-high dose (100mg/kg). ASA was intragastrically administered as a single dose, and an open field test was performed 3 hours later. Subsequently, hippocampus tissues were obtained, and the hippocampus tissue structure was analyzed by analyzing the parameters involved in antioxidant capacity, inflammation, apoptosis, and memory. ASA, especially at moderate doses, increased antioxidant capacity and partially reduced inflammation and apoptotic damage. At high doses, the opposite effect was observed, and the damage levels. Similar effects were detected by histological examination. Although there were no structural defects at low or moderate doses, structural defects were observed at high doses. Although there was no difference in the open field test findings between the groups, the time spent in the center, distance traveled, and speed was slightly higher in the ASA moderate-dose group. In conclusion, ASA may contribute to the improvement of cognitive function at low and moderate doses. However, high doses may cause cognitive impairment.

Keywords

Apoptosis, Aspirin, Behavioral disorders, Inflammation, NSAIDs

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