DETERMINATION OF ANTIMICROBIAL ACTIVITY OF THE DYED SILK FABRICS WITH SOME NATURAL DYES

Year 2015, , 36 - 43, 31.03.2015

Rezan Alkan Emine Torgan Canan Aydın Recep Karadag

https://doi.org/10.7216/130075992015229706

Cited By: 5

Abstract

In this study, silk fabric is dyed with natural indigo. Dyed silk fabric with natural indigo was cut in the 20x20 cm2 size. Excluding a fabric, all fabrics were mordanted in the same percentage with alum metal (KAl(SO4)2.12H2O). Then, silk fabrics for green color dyeing are dyed separately with weld (Reseda luteola), gall oak (Quercus infectoria Olivier) and together weld (Reseda luteola) and gall oak (Quercus infectoria) in different percentage. Antimicrobial functionality of the twenty seven silk fabrics is established. Tests were conducted against the Staphylococcus aureus ATCC 6538. The results of the counting test showed more reduction of survival Staphylococcus aureus in dark-colored fabric. The number of survival microorganism was determined by counting the colonies as colony-forming unit (CFU/ml) and reduction rate of bacteria was calculated. Coloring compounds and their percentages in the natural dyed silk fabrics are detected by HPLC-PDA (high performance liquid chromatography with diode array detection). Colour measurement is done of the dyed silk fabrics by CIEL*a*b* spectrophotometer.

Keywords

Antimicrobial testing, natural dyeing, Staphylococcus aureus, HPLC-PDA, colour measurement

BAZI DOĞAL BOYALAR KULLANILARAK BOYANMIŞ İPEK KUMAŞLARIN ANTİMİKROBİYAL AKTİVİTESİNİN BELİRLENMESİ

Abstract

Bu çalışmada, ipek kumaş doğal indigo bitkisi ile boyanmıştır. Sonra 20x20 cm2 boyutunda kesilmiştir. Bir kumaş hariç tüm kumaşlar aynı yüzdede şap (KAl(SO4)2.12H2O) çözeltisi ile mordanlanmıştır. Yeşil renk boyama için kumaşlar önce ayrı ayrı muhabbet çiçeği (Reseda luteola) ve mazı gomalağı (Quercus infectoria Olivier) ile daha sonra iki bitki beraber farklı yüzdelerde kullanılarak boyama yapılmıştır. Antimikrobiyel test 27 adet ipek kumaş için uygulanmıştır. Testler Staphylococcus aureus ATCC 6538 e karşı yapılmıştır. Sayım sonuçları koyu renkli kumaşlarda S.aureus’ un daha fazla azaldığını göstermiştir. Canlı mikroorganizma sayısı CFU/ml olarak koloni sayımı ile belirlenmiştir. Bakteri azalması tayin edilmiştir. Boyama yapılmış ipek kumaşlardaki renk bileşenleri ve onların yüzdeleri HPLC-PDA (yüksek performanslı sıvı kromatografisi) ile renk ölçümleri ise CIEL*a*b* spektorofotometresi yardımıyla belirlenmiştir

Keywords

Antimikrobiyal test, doğal boyama, Staphylococcus aureus, HPLC-PDA, renk ölçümü

References

• http://www.oeko-tex.com/xdesk/ximages/470/16459_100def, (2011), pdf; last accessed, 22 October.
• Surowiec, I., Orska-Gawry, J., Biesaga, M., Trojanowicz, M.,
• Clementi, C., Miliani, C., Romani, A. and Favaro, G., (2006),
• Degano, I., Ribechini, E., Modugno, F. and Colombini, M.P.,
• Erkan, G., Sengul, K. and Kaya, S., J., (2011), Dyeing of White
• Bechtold, T., Turcanu, A., Ganglberger, E. and Geissler, S., (2003), Natural dyes in modern textile dyehouses - how to combine experiences of two centuries to meet the demands of the future, J. Clean. Prod., Vol.11, 499-509.
• Bechtold, T., Mahmud-Ali, A. and Mussak, R., (2007), Natural dyes for textile dyeing: A comparison of methods to assess the quality of Canadian golden rod plant material, Dyes and Pigments., Vol.75, 287-293.
• Bechtold, T., Mahmud-Ali, A. and Mussak, R.A.M., (2007), Reuse of ash-tree (Fraxinus excelsior L.) bark as natural dyes for textile dyeing: process conditions and process stability, Coloration Technology, Vol.123, 271-279.
• Vankar, P.S., Shanker, R. and Verma, A., (2007), Enzymatic natural dyeing of cotton and silk fabrics without metal mordants, J. Clean. Prod., Vol.15, 1441-1450.
• Vankar, P.S., Shanker, R., Mahanta, D. and Tiwari, S.C., (2008), Ecofriendly sonicator dyeing of cotton with Rubia cordifolia Linn. using biomordant, Dyes and Pigments, Vol.76, 207-212.
• Das, D., Maulik, S.R. and Bhattacharya, S.C., (2008), Dyeing of wool and silk with Rheum emodi, Indian J. Fiber Text. Res., Vol.33, 163-170.
• Zarkogianni, M., Mikropoulou, E., Varella, E. and Tsatsaroni, E., (2011), Colour and fastness of natural dyes: revival of traditional dyeing techniques, Color. Technol., Vol.127, 18-27.
• Surowiec, I., Nowik, W. and Trojanowicz, M., (2008), Post- column deprotonation and complexation in HPLC as a tool for identification and structure elucidation of compounds from natural dyes of historical importance, Microchim. Acta, Vol.162, 393-404.
• Deveoglu, O., Torgan, E. and Karadag, R., (2012), The characterisation by liquid chromatography of lake pigments prepared from European buckthorn (Rhamnus cathartica L.), J. Liq. Chrom. Relat. Technol., Vol.35, 331-338.
• Shaid, M., Shahid-ul-Islam, Mohammed, F., (2014), “Recent advancementsin natural dye applications: a review”, J. Clean. Prod., Vol.53, 1-7.
• Zhang, B., Wang, L., Luo, L. and King, M.W., (2013), “Natural dye extracted from Chinese gall-the application of color and antibacterial activity to wool fabric”, J. Clean.Prod., Vol.80, 310-331.
• Baliarsingh, S., Panda, A.K., Jena, J., Das, T. and Das, N.B., (2012), “Exploring Sustainable Technique on Natural Dye Extraction from Native Plants for Textile: Identification of Colourants, Colourimetric Analysis of Dyed yarns and their Antimicrobial Evaluation, J. Clean. Prod., Vol.37, 257-264.
• Meyer, A.S., Heinonen, M. And Frankel, E.N., (1998), “Antioxidant interactions of catechin, cyanidin, caffeic acid, quercetin, and ellagic acid on human LDL oxidant”, Food Chemistry, Vol. 61, No. ½, 71-75.
• Karadag, R., Torgan, E. & Yurdun, T., (2010), “Formation and HPLC analysis of the natural lake pigment obtained from madder (Rubia tinctorum L.)”, Rewiews in Analytical Chemistry, Vol. 29, No. 1, 1-12.
• Halpine, S.M., (1996), “An improved dye and lake pigment analysis method for high performance liquid chromatography and diode-array detector”, Studies in Conservation, Vol.41, No. 9, 731-735.
• Karadag, R. and Dolen, E., (1997), “Examination of historical textiles with dyestuffs analyses by TLC and derivative spectrophometry”, Turk J. Chem, Vol.21, No.2, 126-133.
• Deveoglu, O., Erkan, G., Torgan, E. and Karadag, R., (2012), “The evaluation of procedures for dyeing silk with buckthorn and walloon oak on the basis of colour changes and fastness characteristics, Coloration Technologies, Vol. 129, 223-231.
• Deveoglu, O., Sahinbaskan, B.Y., Torgan, E. and Karadag, R., (2012), “Investigation on colour, fastness properties and HPLC-DAD analysis of silk fibres dyed with Rubia tinctorium L. and Quercusithaburensis Decaisne”, Coloration Technologies, 128, 364-370.