Novel Approach to Controlled Surface Modification in Textile Via Magnetic Cross-Linked Enzyme Aggregates (Clea)

Year 2015, Volume: 22 Issue: 97, 43 - 49, 31.03.2015

Alixander Perzon Cedric Dıcko Özgür Çobanoğlu Onur Yükselen Estera Dey Jitka Eryılmaz

https://doi.org/10.7216/130075992015229707

Abstract

In this proceeding, architecting novel magnetic Cross-Linked-Enzyme-Aggregates (CLEA) nanoparticles to effectively and controllably modify textile surfaces without damaging the fabric itself, will be discussed. The efficiency is due to exponentially increased surface concentration of biocatalyst and to the choice of aggregate size which controls the penetration depth and hence preventing the fabric disruption. In addition, due to the presence of magnetic nanoparticles, the continuous recovery and reusability makes this design more economic and ecologic compared to the conventional chemical processes. The careful fabrication and functionalization of such a design with preliminary results will be presented in this contribution.

Keywords

Biocatalysis, enzymatic process, biomimicking, biostoning, textile surface treatment, cellulosome, clea, magnetic nano-particle, reusability

Tekstilde Çapraz Bağlı Enzim Topluluklarının Kontrollü Yüzey Modifikasyonunda Kullanılması

Abstract

Bu makalede, manyetik nano-parçacıkların da içinde bulunduğu, çapraz bağlı enzim topluluklarının (CLEA) oluşturulması ve tekstil işlemede kullanılması fikri ve uygulaması sunulmuştur. CLEA’nın, serbest enzimlere göre daha verimli olmasının iki temel nedeni vardır. Birincisi ayarlanabilen CLEA büyüklüğü ile enzim etkinliğinin kumaşın gözeneklerine girme miktarı sınırlandırılabilir ki bu, kumaşın mukavemetini artırır. İkincisi ise, enzimlerin kumaşın istenmeyen bölümlerine girişini engelleyerek, yalnızca yüzeye yönlendirilmeleri sayesinde yüzey işlenme veriminin arttırılmasıdır. Bunlara ek olarak, CLEA içine yerleştirilmiş manyetik nano-parçacıklar CLEA’nın tekrar tekrar kullanılmasına imkan sunar. Önerilen süreç, alışılagelmiş kimyasal süreçlerle karşılaştırıldığında daha tutumlu ve çevrecidir. İlk CLEA tasarımımız ve uygulamaları, öncül ölçüm sonuçları ile beraber sunulmuştur

Keywords

Biyokataliz, enzimatik proses, biyo-taklit etme, biyotaşlama, tekstil yüzey muamelesi, selülozom, clea, manyetik nano-partikül, tekrar kullanılabilirilik

References

• Mojsov, K. (2011), Application of enzymes in the textile industry: a review. in II International Congress "Engineering, Ecology and Materials in the Processing Industry". Jahorina, Bosnia and Hercegovina: UGD Academic Repository.
• Cavaco-Paulo, A., Gubitz G., eds. (2003), Textile processing with enzymes. Woodhead Publishing Ltd: Cambridge England.
• Schmitt B., Prasad A.K., (1998), Update of indigo denim washing. Colourage, 45(10): p. 20-24.
• Bhat M.K., (2000),Cellulases and related enzymes in biotechnology. Biotechnology Advances, 18(5): p. 355-383.
• Cavaco-Paulo A., (1998), Mechanism of cellulse action in textile processes. Carbohydrate Polymers, 37: p. 273-277.
• Lenting H.B.M., Warmoeskerken M.M.C.G., (2001), Guidelines to come to minimized tensile strength loss upon cellulase application. Journal of Biotechnology, 89(2): p. 227-232.
• Klahorst S., Kumar A., Mullins M.M., (1994), Optimizing the use of cellulase enzymes. Textile Chemist and Colorist, 26(2): p. 13-18.
• Cavaco-Paulo A., Morgado J., Almeida L., and Kilburn D., (2000), Indigo backstaining during cellulase washing. Textile Research Journal, 68(6): p. 398-401.
• Gusakov A.V., Sinitsyn P., Markov A. V., Skomarovsky A. A., Sinitsyna O. A., Berlin A. G., and Ankudimova N. V., (2000), Indigo-binding domains in cellulase molecules. Biocatalysis- 2000: Fundamentals and Applications, (095): p. 77-80.
• Pazarlioğlu N.K., Sariişik M., Telefoncu A., (2005), Treating denim fabrics with immobilized commercial cellulases. Process Biochemistry, 40(2): p. 767-771.
• Popdrepsek G.H., Primožiþ M., Knez Ž., Habulin M., (2012), Immobilization of cellulase for industrial production, in Chemical Engineering Transactions. p. 235-240.
• Yu Y., Yuan J., Wang Q., Fan X., Wang P., Cui L., (2001), A promising approach for bio-finishing of cotton using immobilized acid-cellulase. Fibers and Polymers, 15(5): p. 932-937.
• Cao L., (2005),Immobilised enzymes: science or art? Current opinion in chemical biology, 9(2): p. 217-26.
• Hanefeld U., Gardossi L., and Magner E., (2009), Understanding enzyme immobilisation. Chemical Society Reviews, 38(2): p. 453-68.
• Dalal S., Sharma A., Gupta M.N., (2007), A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activities. Chemistry Central Journal, 1(1): p. 16-16.
• Broun G.H., (1976), Chemically aggregated enzymes, in Method in Enzymology, K. Mosbach, Editor. Academic Press: New York. p. 263-580.
• Reza R.T., Pérez C.A.M., González C. A. R., Romero H. M., Casillas P. E. G.., (2010), Effect of the polymeric coating over Fe3O4 particles used for magnetic separation. Central European Journal of Chemistry, 8(5): p. 1041-1046.
• Talekar S., Ghodake V., Ghotage T., Rathod P., Deshmukh P., Nadar S., Mulla M., Ladole M., (2012), Novel magnetic cross-linked enzyme aggregates (magnetic CLEAs) of alpha amylase. Bioresource Technology, 123: p. 542-547.
• Sandip B., Bule M.V., Singhal R.S., Ananthanarayan L., (2009), Glucose oxidase — An overview. Biotechnology Advances, 27: p. 489-501.
• Gusakov A., Sinitsyn A.P., Berlin A.G., Markov A.V., Ankudimova N.V., (2000), Surface hydrophobic amino acid residues in cellulase molecules as a structural factor responsible for their high denim-washing performance. Enzyme and microbial technology, 27(9): p. 664-671.
• Gusakov A.V., Sinitsyn A.P., (2000), Indigo-binding domains in cellulase molecules. Biocatalysis-2000: Fundamentals and Applications, 41(6): p. 77-80.
• Markov A.V., Gusakov V., Kondratyeva E. G., Okunev O. N., Bekkarevich A. O., Sinitsyn A. P., (2005), New effective method for analysis of the component composition of enzyme complexes from Trichoderma reesei. Biochemistry. Biokhimii͡a, 70(6): p. 657-63.
• Sinitsyn A.P., Gusakov, A.V., Grishutin, S.G., Sinitsyna, O.A., Ankudimova, N.V, (2001),Application of microassays for investigation of cellulase abrasive activity and backstaining. Journal of Biotechnology, 89(2-3): p. 233-8.