Soğuk Stresi Uygulamasının Ve Hipotansif Bir İlaç Olan Enalapril Maleat ’ın Bazı Sıçan Dokularında Total Rna Seviyelerine Etkileri

Öz

Bu çalışmada kontrol grubu, soğuk stres, enalapril maleat ve soğuk stres ile beraber enalapril maleatuygulamalarının Fischer-344 sıçanlarının adrenal medulla, hipotalamus ve kalpte total RNA düzeyleri üzerineetkileri araştırılmıştır. Stres hormonlarının kontrolünde ve salınmasında etkili enzimlerin düzeyleri hakkında ön bilgisağlayacağı düşüncesi ile total RNA düzeylerinin saptanması amaçlanmıştır. Soğuk stresi uygulamasında sıçanlar48 saat süreyle +8°C’de soğuğa maruz bırakılmıştır. Enalapril maleat, 10 mg/kg olacak şekilde sıçanlaraintraperitonal olarak yapılmıştır. Soğuk stresi ile beraber enalapril maleat uygulamasında, sıçanlara 10 mg/kgolacak şekilde intraperitonal olarak enalapril uygulandıktan sonra bu sıçanlar 48 saat +8°C’de soğuğa maruzbırakılmıştır. Uygulamalardan sonra sıçanlardan adrenal medulla, hipotalamus ve kalp doku örnekleri alınmıştır. Herüç organın doku örneğinde soğuk stresi uygulaması sonucu, total RNA seviyesinde istatistiksel olarak anlamlı artışolduğu gözlenmiştir p0.05 . Kalpte ise total RNA seviyesindeistatistiksel olarak anlamlı artışlar gözlenmiştir p

Anahtar Kelimeler

• Soğuk stresi, enalapril maleat, total RNA, sıçan

The Effects Of Cold Stress Treatment And Enalapril Maleat Which Is A Hypotensive Drug On Total Rna Levels In Different Rat Tissues

Abstract

In this study, the effects of cold stress, enalapril maleat and enalapril maleate accompanied to cold stresstreatments on total RNA levels of adrenal medulla, hypotalamus and heart tissues of Fischer -344 rats have beenobserved. Determination of total RNA levels was aimed for being able to get introductory information about theenzyme levels which play a central role in the control and secretion of stress hormons. In cold stress treatment, rats were exposed to +8°C temperature during 48 hours. Enalapril maleate wasinjected intraperitoneally 10 mg/kg body weight . In cold stress accompanied to enalapril maleat treatment, enalaprilmaleat was injected in doses of 10 mg/kg per rat, after injection, rats were exposed to +8°C during 48 hours. Afterthe treatments, tissue samples of adrenal medulla, hypotalamus and heart were taken.In all three these tissues, elevations in total RNA levels were found to be statistically significant because of coldstress treatment p0.05 . On the other hand, increase in total RNA levelsof heart tissue had been found statistically significant p

Keywords

• Cold stress, enalapril maleat, total RNA, rat

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