Soğuk Stresi Uygulamasının Ve Hipotansif Bir İlaç Olan Enalapril Maleat ’ın Bazı Sıçan Dokularında Total Rna Seviyelerine Etkileri

Öz

Bu çalışmada kontrol grubu, soğuk stres, enalapril maleat ve soğuk stres ile beraber enalapril maleatuygulamalarının Fischer-344 sıçanlarının adrenal medulla, hipotalamus ve kalpte total RNA düzeyleri üzerineetkileri araştırılmıştır. Stres hormonlarının kontrolünde ve salınmasında etkili enzimlerin düzeyleri hakkında ön bilgisağlayacağı düşüncesi ile total RNA düzeylerinin saptanması amaçlanmıştır. Soğuk stresi uygulamasında sıçanlar48 saat süreyle +8°C’de soğuğa maruz bırakılmıştır. Enalapril maleat, 10 mg/kg olacak şekilde sıçanlaraintraperitonal olarak yapılmıştır. Soğuk stresi ile beraber enalapril maleat uygulamasında, sıçanlara 10 mg/kgolacak şekilde intraperitonal olarak enalapril uygulandıktan sonra bu sıçanlar 48 saat +8°C’de soğuğa maruzbırakılmıştır. Uygulamalardan sonra sıçanlardan adrenal medulla, hipotalamus ve kalp doku örnekleri alınmıştır. Herüç organın doku örneğinde soğuk stresi uygulaması sonucu, total RNA seviyesinde istatistiksel olarak anlamlı artışolduğu gözlenmiştir p0.05 . Kalpte ise total RNA seviyesindeistatistiksel olarak anlamlı artışlar gözlenmiştir p

Anahtar Kelimeler

• Soğuk stresi, enalapril maleat, total RNA, sıçan

The Effects Of Cold Stress Treatment And Enalapril Maleat Which Is A Hypotensive Drug On Total Rna Levels In Different Rat Tissues

Abstract

In this study, the effects of cold stress, enalapril maleat and enalapril maleate accompanied to cold stresstreatments on total RNA levels of adrenal medulla, hypotalamus and heart tissues of Fischer -344 rats have beenobserved. Determination of total RNA levels was aimed for being able to get introductory information about theenzyme levels which play a central role in the control and secretion of stress hormons. In cold stress treatment, rats were exposed to +8°C temperature during 48 hours. Enalapril maleate wasinjected intraperitoneally 10 mg/kg body weight . In cold stress accompanied to enalapril maleat treatment, enalaprilmaleat was injected in doses of 10 mg/kg per rat, after injection, rats were exposed to +8°C during 48 hours. Afterthe treatments, tissue samples of adrenal medulla, hypotalamus and heart were taken.In all three these tissues, elevations in total RNA levels were found to be statistically significant because of coldstress treatment p0.05 . On the other hand, increase in total RNA levelsof heart tissue had been found statistically significant p

Keywords

• Cold stress, enalapril maleat, total RNA, rat

Kaynakça

1. 1. Devlin TM. Textbook of Biochemistry. Hahnemann University School of Medicine A. Wiley Medical Publication. New York ,1986.
2. 2. Fregly MJ, Kikta DC, Threatte RM, Torres JL, Barney CC. Development of hypertension in rats during chronic exposure to cold. J Appl Physiol 1989; 66 (2): 741-9.
3. 3. Sun Z, Cade JR, Fregly MJ. Cold-induced hypertension. A model of mineralocorticoid-induced hypertension. Ann N Y Acad Sci 1997; 813: 682-8.
4. 4. Murat SN, Ecemiş ZA, Angiotensin Konverting Enzim İnhibitörleri ve Klinik Kullanımı. Türkiye Tıp Dergisi 1995; Sayı: 3, Cilt: 2
5. 5. Birincioğlu M, Aksoy T, Ölmez E, et al. Protective Effect of ACE Inhibitors on Ischemia-Reperfusion-induced Arrhythmias in Rats: Is this Effect Related to the Free Radical Scavenging Action of these Drugs?. Free Rad Res 1997; 27 (4): 389-96.
6. 6. Noyan A, Fizyoloji Ders Kitabı. 8. Baskı, Ankara: Anadolu Üniversitesi Yayınları. No: 2 Meteksan, 1980.
7. 7. Katzung BG. Temel ve Klinik Farmakoloji Cilt I, İstanbul : Barış Kitabevi, 1995.
8. 8. Opie LH. Angiotensin- Converting Enzyme: Scientific Basis for Clinical Use. John Wiley& Sons’s Pres, 1994.

9. 9. Tümer N, and La Rochelle JS. Tyrosine Hydroxylase Expression in Rat Adrenal Medulla: İnfluence Influence of age and cold. Pharmacol Biochem Behave 1995; 51 (4): 775-80.
10. 10. Chomzynski P, Sacchi N. Single–Step Method of RNA Isolation by Acid Guanidinium Thiocyanatephenol – Chloroform Extraction. Anal Bio Chem 1987; 162: 156-9.
11. 11. Roberts J, Tumer N. Age related changes in autonomic function of catecholamines. Rewiew of Biological in Aging 1987; 3: 257-98 .
12. 12. Reynolds JEF, Martindale. The Extra Pharmacopoeia. Thirty first Edition. Pharmaceutical Society. London Royal, 1996.
13. 13. Miner LL, Baruchin A, and Kaplan BB. Effect of Cold Stress on cholinergic receptors in the rat adrenal gland. Neuroscı Lett 1989; 106(3): 339-44.
14. 14. Fregly MJ, Rossi F, Sun Z, et al. Effect of Chronic Treatment with Prazosin and L- Arjinine on the Elevation of Blood Pressure during Cold Exposure. Pharmacol 1994; 49(6): 351-62.
15. 15. Tümer N, Bowman CJ, La Rochelle JS, et al. Induction of tyrosine hydroxylase by forskolin: Modulation with age. Eur J Pharmacol 1997; 324: 57-62.
16. 16. Axelrod J, Mueller RA, Henry JP, et al. Changes in enzymes involved in the biosynthesis and metabolism of noradrenaline and adrenaline after psychosocial stimulation. Nature 1970; 225:1059-60.
17. 17. Smith MA, Brady LS, Glowa J, Gold PW and Herkenham M. Effects of stres and adrenalectomy on tyrosine hydroxylase mRNA levels in the locus ceruleus by in situ hybridization. Brain Res 1991; 544: 26-32.
18. 18. Stachowiak MK, Fluharty SJ, Stricker EM, Zigmond MJ, Kaplan BB. Molecular adaptations in catecholamine biosynthesis induced by cold stress and sympathectomy. J Neurosci Res. 1986; 16 (1): 13-24.
19. 19. Baruchin A, Vollmer RR, Miner LL, Sell SL, Stricker EM, Kaplan BB. Cold-induced increases in phenylethanolamine N-methyltransferase (PNMT) mRNA are mediated by non-cholinergic mechanisms in the rat adrenal gland. Neurochem Res. 1993 Jul; 18 (7): 759-66.
20. 20. Baruchin A, Weisberg EP, Miner LL, Ennis D, Nisenbaum LK, Naylor E, Stricker EM, Zigmond MJ, Kaplan BB. Effects of cold exposure on rat adrenal tyrosine hydroxylase: an analysis of RNA, protein, enzyme activity, and cofactor levels. J Neurochem. 1990 May; 54 (5): 1769-75.