Gıda mikrobiyolojisinde Enterobacteriaceae üyeleri için kromojenik ve florojenik besiyerleri

Öz

Gıda endüstrisinde kalite güvencenin sağlanmasında ve halk sağlığının korunmasında mikrobiyolojik risklerin hızlı tespiti önemlidir. Bu nedenle gıda mikrobiyolojisinde analiz sürelerini kısaltmak amacıyla birçok alternatif metot geliştirilmiştir. Bu metotların birçoğu mikroorganizmaların spesifik enzim aktivitelerini tespit etmeye yönelik kromojenik ve florojenik substratların kullanımı esasına dayalıdır. Gıda mikrobiyolojisi laboratuvarlarında iş gücü ve zamandan tasarruf sağladıklarından dolayı kromojenik ve florojenik substratlar içeren besiyerlerinin kullanımı artarak devam etmektedir. Bu derlemede, gıda mikrobiyolojisi bakımından en önemli familya olan Enterobacteriaceae familyası üyesi bakterilerin tespit edilmesi ve sayılmasına yönelik kromojenik ve florojenik besiyerleri son gelişmeler doğrultusunda kapsamlı olarak ele alınmıştır.

Anahtar Kelimeler

• Kromojenik besiyeri, florojenik besiyeri, Enterobacteriaceae, gıda mikrobiyolojisi

Chromogenic and fluorogenic media for members of Enterobacteriaceae in food microbiology

Abstract

Fast detection of microbiological risks is important for quality assurance in the food industry and public health. For this reason, several alternative methods have been developed to reduce the analysis period in food microbiology. Most of these methods are based on the utilization of chromogenic or fluorogenic substrates for the detection of specific enzyme activities of microorganisms. In food microbiology laboratories, use of media with chromogenic and fluorogenic substrates has continually increased due to cost, labor, and time savings features. This review discusses chromogenic and fluorogenic media for analysing members of Enterobacteriaceae, the most important family in food microbiology, in respect of recent devolopments. According to results of the latest studies, performance, advantage and disadvantage of chromogenic and fluorogenic media were evaluated

Keywords

• Chromogenic medium, fluorogenic medium, Enterobacteriaceae, food microbiology

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