Kanatlı eti ürünlerinde toplam aerobik mezofilik mikroorganizma ve Enterobacteriaceae belirlenmesinde klasik ve hızlı yöntemlerin karşılaştırması

Öz

Amaç: Bu çalışmada, bütün karkas ve mekanik olarak ayrılmış 123 adet kanatlı et ürününde, toplam aerobik mezofilik mikroorganizma sayısı ve Enterobacteriaceae sayısının belirlenmesinde klasik ve hızlı test yöntemlerinin karşılaştırılması amaçlanmıştır. Yöntemler: Çalışmada, toplam aerobik mezofilik mikroorganizma sayısının belirlenmesinde “ISO 4833: 2003” klasik yöntemi ve “TEMPO TVC” hızlı test yöntemi, Enterobacteriaceae sayısının belirlenmesinde ise “ISO 21528-2: 2004” klasik yöntemi ve “TEMPO EB” hızlı test yöntemi eş zamanlı olarak çalışılmıştır. Buna göre, toplam aerobik mezofilik mikroorganizma sayımında 100, Enterobacteriaceae sayımında ise 85 örneğe ait sonuçlar istatistikî olarak değerlendirilmiştir. Klasik ve hızlı test yöntemlerinin istatistiki olarak karşılaştırılmasında office excel 2007 Microsoft, Redmond, ABD programı kullanılarak, tanımlayıcı istatistik testleri ve F-testi yapılmıştır. Lineer regresyon ve Pearson korelasyon analizleri ise MINITAB 16 programı Minitab Inc., State College, TX, ABD kullanılarak yapılmıştır.Results: According to the results of the study, it was determined that there was no statistically significant

Anahtar Kelimeler

• kanatlı eti, Enterobacteriaceae, toplam aerobik mezofilik mikroorganizma, mikrobiyolojik teknikler

Comparison of conventional and rapid methods for determination of total aerobic mesophilic microorganisms and Enterobacteriaceae in poultry products

Abstract

Objective: In this study, it was aimed to compare the conventional and rapid test methods in determining the numbers of both total aerobic mesophilic microorganizm and Enterobacteriaceae in totally 123 poultry products which were both whole carcass and mechanically separated.Methods: In In the study, it was simultaneously used ISO 4833:2003 conventional method and TEMPO TVC rapid test method for determining the number of total aerobic mesophilic microorganism, as well as ISO 21528-2:2004 conventional method and TEMPO EB rapid test method for the enumeration of Enterobacteriaceae. According to this, it was statistically evaluated the results belonging to 100 samples in a total of aerobic mesophilic microorganism count and also 85 samples in Enterobacteriaceae count. Descriptive statistical test and F-test were performed by using office excel 2007 Software Microsoft, Redmond, USA at the statistical comparison of the conventional and rapid test methods. In addition, linear regression and Pearson correlation analyses were performed by using MINITAB 16 software Minitab Inc., State College, TX, USA .Results: According to the results of the study, it was determined that there was no statistically significant

Keywords

• poultry, Enterobacteriaceae, total aerobic mesophilic microroganism, microbiological techniques

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