Kahramanmaraş yöresinde farklı yaş gruplarındaki bireylerde antioksidan enzimlerinden süperoksit dismutaz (sod), katalaz (cat) ve glukoz-6-fosfat dehıdrogenaz (g6pd) aktivitelerinin taranması

Abstract

Amaç: Bu çalışma Kahramanmaraş yöresinde ikamet eden sağlıklı bireylerde yaşlarına göre eritrosit metabolizmasında yer alan antioksidan enzimlerin aktivitelerini tespit etmek amacı ile yapılmıştır. Gereç ve Yöntemler: Eritrosit metabolizmasında yer alan Glukoz-6-fosfat Dehidrogenaz (G6PD), Süperoksit Dismutaz (SOD) ve Katalaz (CAT) enzimlerinin yokluğunu veya yetersizliğini tespit etmek maksadıyla 0-125 yaşları arasında 281 sağlıklı birey çalışmaya dahil edilmiştir. Çalışma grubuna alınan bireylerin hematolojik verileri normal değerdedir. Bulgular: G6PD ve CAT aktiviteleri Beutler Metodu ile SOD aktivitesi Fridovich Metodu ile saptanmıştır. Üç bireyde G6PD enzim eksikliği (sıfır aktivite) ve yedi bireyde katalaz enzim yetersizliği saptanmıştır. Sonuç: Bu çalışmada yaşlanma ile G6PD ve CAT aktivitelerinin arttığı, SOD aktivitesinin ise azaldığı (p<0,05) ve aynı yaş gruplarında cinsiyete bağlı olarak enzim aktivitelerinin farklılık göstermediği saptanmıştır (p>0,05). Sonuç olarak, yaşlanma ile oksidatif stres arasında pozitif bir korelasyon olduğu kanısına varılmıştır.

Keywords

• Yaşlanma,
• SOD,
• CAT,

Thanks

ksü tıbbi biyokimya bölümü çalışanları ve pek kıymetli hocam ergül belge kurutaş hanımefendiye teşekkür ederim.

Screenıng of antıoxıdant enzymes superoxıde dısmutase (sod), catalase (cat) and glucose-6-phosphate dehydrogenase (g6pd) actıvıtıes ın persons of dıfferent age groups ın kahramanmaras regıon

Abstract

Aim: This study was carried out to determine the activities of antioxidant enzymes in erythrocyte metabolism in healthy individuals residing in Kahramanmaraş region according to their age. Material and Methods: In order to detect the absence or insufficiency of Glucose-6-phosphate Dehydrogenase (G6PD), Superoxide Dismutase (SOD) and Catalase (CAT) enzymes involved in erythrocyte metabolism, 281 healthy individuals between the ages of 0-125 were included in the study. Hematological data of the individuals included in the study group were normal. Results: G6PD and CAT activities were determined by Beutler Method and SOD activity was determined by Fridovich Method. G6PD enzyme deficiency (zero activity) was found in three individuals and catalase enzyme deficiency was found in seven individuals. Conclusion: In this study; G6PD and CAT activities increased with aging, while SOD activity decreased (p<0.05) and enzyme activities did not differ depending on gender in the same age groups (p>0.05). As a result, it was concluded that there is a positive correlation between aging and oxidative stress.

Keywords

• Aging
• SOD
• CAT
• G6PD

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