Kahramanmaraş yöresinde farklı yaş gruplarındaki bireylerde antioksidan enzimlerinden süperoksit dismutaz (sod), katalaz (cat) ve glukoz-6-fosfat dehıdrogenaz (g6pd) aktivitelerinin taranması

Öz

Amaç: Bu çalışma Kahramanmaraş yöresinde ikamet eden sağlıklı bireylerde yaşlarına göre eritrosit metabolizmasında yer alan antioksidan enzimlerin aktivitelerini tespit etmek amacı ile yapılmıştır. Gereç ve Yöntemler: Eritrosit metabolizmasında yer alan Glukoz-6-fosfat Dehidrogenaz (G6PD), Süperoksit Dismutaz (SOD) ve Katalaz (CAT) enzimlerinin yokluğunu veya yetersizliğini tespit etmek maksadıyla 0-125 yaşları arasında 281 sağlıklı birey çalışmaya dahil edilmiştir. Çalışma grubuna alınan bireylerin hematolojik verileri normal değerdedir. Bulgular: G6PD ve CAT aktiviteleri Beutler Metodu ile SOD aktivitesi Fridovich Metodu ile saptanmıştır. Üç bireyde G6PD enzim eksikliği (sıfır aktivite) ve yedi bireyde katalaz enzim yetersizliği saptanmıştır. Sonuç: Bu çalışmada yaşlanma ile G6PD ve CAT aktivitelerinin arttığı, SOD aktivitesinin ise azaldığı (p<0,05) ve aynı yaş gruplarında cinsiyete bağlı olarak enzim aktivitelerinin farklılık göstermediği saptanmıştır (p>0,05). Sonuç olarak, yaşlanma ile oksidatif stres arasında pozitif bir korelasyon olduğu kanısına varılmıştır.

Anahtar Kelimeler

• Yaşlanma,
• SOD,
• CAT,

Screenıng of antıoxıdant enzymes superoxıde dısmutase (sod), catalase (cat) and glucose-6-phosphate dehydrogenase (g6pd) actıvıtıes ın persons of dıfferent age groups ın kahramanmaras regıon

Öz

Aim: This study was carried out to determine the activities of antioxidant enzymes in erythrocyte metabolism in healthy individuals residing in Kahramanmaraş region according to their age. Material and Methods: In order to detect the absence or insufficiency of Glucose-6-phosphate Dehydrogenase (G6PD), Superoxide Dismutase (SOD) and Catalase (CAT) enzymes involved in erythrocyte metabolism, 281 healthy individuals between the ages of 0-125 were included in the study. Hematological data of the individuals included in the study group were normal. Results: G6PD and CAT activities were determined by Beutler Method and SOD activity was determined by Fridovich Method. G6PD enzyme deficiency (zero activity) was found in three individuals and catalase enzyme deficiency was found in seven individuals. Conclusion: In this study; G6PD and CAT activities increased with aging, while SOD activity decreased (p<0.05) and enzyme activities did not differ depending on gender in the same age groups (p>0.05). As a result, it was concluded that there is a positive correlation between aging and oxidative stress.

Anahtar Kelimeler

• Aging
• SOD
• CAT
• G6PD

Kaynakça

1. 1. Hou Y, Dan X, Babbar M, et al. Ageing as a risk factor for neurodegenerative disease. Nat Rev Neurol 2019; 15: 565-81.
2. 2. Tümerdem Y. Real age. Turk J Geriatr 2006; 9: 195-196.
3. 3. WHO; US National Institute on Aging; National Institutes of Health; Global Health and Ageing. Available from: https://www. who.int/ageing/ publications/ global_health/en/ 2011.
4. 4. Benz CC, Yau C. Ageing, oxidative stress and cancer: Paradigms in parallax. Nat Rev Cancer 2008; 8: 875-9.
5. 5. Karasu Ç. Theories of biological aging: role of oxidative stres. Turkiye Klinikleri J Med Sci 2008; 28 : 1-11.
6. 6. Cheeseman KH, Slater TF. An Introduction to Free Radical Biochemistry. Br Med Bull, 1993; 49: 481-93.
7. 7. Kurutas EB. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state, Nutrition journal 2016;15 :1-22.
8. 8. Ighodaro OM, Akinloye OA. " First-line defense antioxidants— superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX): essential roles in the entire antioxidant defense grid.," Alexandria Journal of Medicine 2018; 54: 287-93.

9. 9. Antwi-Baffour S, Adjei JK, Forson PO, et al. Comorbidity of Glucose- 6-Phosphate Dehydrogenase Deficiency and Sickle Cell Disease Exert Significant Effect on RBC Indices. Anemia 2019; 2019: 3179173.
10. 10. Yang WC, Tai S, Hsu CL, et al. Reference levels for glucose-6- phosphate dehydrogenase enzyme activity in infants 7-90 days old in Taiwan. J Formos Med Assoc 2020; 119 :69-74.
11. 11. Ong KIC, Iwagami M, Araki H, et al. Prevalence of G6PD Viangchan variant in endemic malaria areas in Lao PDR: an outcome for malaria eradication by 2030. Malar J 2019; 18:75
12. 12. Pes GM, Parodi G, Dore MP. Glikoz-6-fosfat dehidrojenaz eksikliği ve kardiyovasküler hastalık riski:Eğilim skoru eşleştirilmiş bir çalışma. Ateroskleroz. 2019; 282 :148-53.
13. 13. Ninfali P, Orsenigo T, Barociani, Papa S. “Rapid purification of gucose 6-phosphate dehydrogenase from mammal’s erythrocytes”. Preparative Biochemistry 20; 1990: 297-309.
14. 14. Beutler E. Red Cell Metabolism. A Manual of Biochemical Methods. 2nd ed. New York 1984.
15. 15. Fridovich I. Superoxide dismutase. Adv Enzymol 1974.
16. 16. Whıte WL, Erıckson M, Stewens, S.C. Determination of Total Protein and Albümin Chemistry for Clinical Laborotory. 4th. Ed. P. 183 The C. V. Mosby Company, St Louis, Mo.1976.
17. 17. Hatao H. Effects of Acute Exercise on Lung Antioxidant Enzymes in Young and Old Rats. Mechanisms of Ageing and Development 2006; 127: 384-90.
18. 18. Yargıçoğlu P, Şahin E, Gümüşlü S, Ağar A. The Effect of Sulfur Dioxide Inhalation on Active Learning Antioxidant Status and Lipid Peroxidation During Aging. Neurotoxicology and Teratology 2007; 29: 211-8.
19. 19. Bayne AV, Sohal RS. Effects of Superoxide Dismutase\Catalase Mimetics on Life Span and Oxidative Stress Resistance in The Housefly, Musca Domestica. Free Radical Biology & Medicine 2002; 32: 1229-34.
20. 20. Devı SA, Kıran TR. Regional Responses in Antioxidant System to Exercise Training and Disetary Vitamin E in Aging Rat Brain. Neurobiology of Aging 2004; 25: 501-8.
21. 21. İnal ME, Kanbak G, Sunal E. Antioxidant Enzyme Activities and Malondialdehyde Levels Related to Aging. Clinica Chimica Acta 2004; 305: 75-80.
22. 22. Wickens AP. Ageing and the free fadical theory. Respiration Physiology 2001; 128: 379391.
23. 23. Tolmasoff JM, Ono T, Cutler RG. Superoxide dismutase: correlation with life span and specific metabolic rate in primate species. Proceding of the National Academy of Science 1980; 77: 2777-81.
24. 24. Jolıtha AB, Subramanyam MVV, Devı SA. Modification by Vitamin E and Exercise of Oxidative Stres in Regions of Aging Rat Brain: Studies on Superoxide Dismutase Isoenzymes and Protein Oxidation Status. Experimental Gerontoloji 2006;41: 753-763.
25. 25. Lı Y, Qın H, Chen Q, Wang J. Neurochemical and Behavioral Effects of The Intrahippocampal Co-Injection of B-Amyloid Protein 1-40 and Ibotenic Acid in Rats. Life Sciences, 2005; 76: 1189-1197.
26. 26. Koyu A, Özgüner F, Çalışkan S, Karaca H. Preventive Effect of Vitamin E on Iron-Induced Oxidative Damage in Rabbit. SAGE Journals 2005; 21: 239.
27. 27. Beutler E. Glucose-6-phosphate dehydrogenase deficiency: a historical perspective. Blood 2008; 11: 16-24.
28. 28. Mesner O, Hammerman C, Goldschmidt D, et al. Glucose-6- phosphate dehydrogenase activity in male premature and term neonates. Arch Dis Child Fetal Neonatal Ed 2004; 89: 555-7.
29. 29. Reglinski J, Hoey J, Simith WE, Sturrock RD. Celluler response to oxidative stress at sulfhidryl group receptor sites on the erytrocyte membrane. J Biol Chem 1988; 263: 12360-6.