Kahramanmaraş yöresinde farklı yaş gruplarındaki bireylerde antioksidan enzimlerinden süperoksit dismutaz (sod), katalaz (cat) ve glukoz-6-fosfat dehıdrogenaz (g6pd) aktivitelerinin taranması

Yıl 2022, , 285 - 289, 30.06.2022

İlter Demirhan , Ergul Belge Kurutas

https://doi.org/10.18663/tjcl.994531

Öz

Amaç: Bu çalışma Kahramanmaraş yöresinde ikamet eden sağlıklı bireylerde yaşlarına göre eritrosit metabolizmasında yer
alan antioksidan enzimlerin aktivitelerini tespit etmek amacı ile yapılmıştır.
Gereç ve Yöntemler: Eritrosit metabolizmasında yer alan Glukoz-6-fosfat Dehidrogenaz (G6PD), Süperoksit Dismutaz
(SOD) ve Katalaz (CAT) enzimlerinin yokluğunu veya yetersizliğini tespit etmek maksadıyla 0-125 yaşları arasında 281
sağlıklı birey çalışmaya dahil edilmiştir. Çalışma grubuna alınan bireylerin hematolojik verileri normal değerdedir.
Bulgular: G6PD ve CAT aktiviteleri Beutler Metodu ile SOD aktivitesi Fridovich Metodu ile saptanmıştır. Üç bireyde G6PD
enzim eksikliği (sıfır aktivite) ve yedi bireyde katalaz enzim yetersizliği saptanmıştır.
Sonuç: Bu çalışmada yaşlanma ile G6PD ve CAT aktivitelerinin arttığı, SOD aktivitesinin ise azaldığı (p<0,05) ve aynı yaş
gruplarında cinsiyete bağlı olarak enzim aktivitelerinin farklılık göstermediği saptanmıştır (p>0,05). Sonuç olarak, yaşlanma
ile oksidatif stres arasında pozitif bir korelasyon olduğu kanısına varılmıştır.

Anahtar Kelimeler

Yaşlanma,, SOD,, CAT,

Teşekkür

ksü tıbbi biyokimya bölümü çalışanları ve pek kıymetli hocam ergül belge kurutaş hanımefendiye teşekkür ederim.

Screenıng of antıoxıdant enzymes superoxıde dısmutase (sod), catalase (cat) and glucose-6-phosphate dehydrogenase (g6pd) actıvıtıes ın persons of dıfferent age groups ın kahramanmaras regıon

Öz

Aim: This study was carried out to determine the activities of antioxidant enzymes in erythrocyte metabolism in healthy
individuals residing in Kahramanmaraş region according to their age.
Material and Methods: In order to detect the absence or insufficiency of Glucose-6-phosphate Dehydrogenase (G6PD),
Superoxide Dismutase (SOD) and Catalase (CAT) enzymes involved in erythrocyte metabolism, 281 healthy individuals
between the ages of 0-125 were included in the study. Hematological data of the individuals included in the study group
were normal.
Results: G6PD and CAT activities were determined by Beutler Method and SOD activity was determined by Fridovich
Method. G6PD enzyme deficiency (zero activity) was found in three individuals and catalase enzyme deficiency was found
in seven individuals.
Conclusion: In this study; G6PD and CAT activities increased with aging, while SOD activity decreased (p<0.05) and
enzyme activities did not differ depending on gender in the same age groups (p>0.05). As a result, it was concluded
that there is a positive correlation between aging and oxidative stress.

Anahtar Kelimeler

Aging, SOD, CAT, G6PD

Kaynakça

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