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Antimicrobial effects of ropivacaine, levobupivacaine and bupivacaine at different temperatures

Year 2024, Volume: 15 Issue: 3, 400 - 405, 30.09.2024
https://doi.org/10.18663/tjcl.1523812

Abstract

Aim: Various prior studies have shown a possible antimicrobial activity of different local anesthetic (LA) drugs. The aim of this study was to assess the antimicrobial (antibacterial or antifungal) activity of commonly used commercially available LA drugs used in regional anesthesia practice such as; ropivacaine, levobupivacaine and bupivacaine on S.aureus ATCC 6538, S. epidermidis, E.coli and Candida albicans at different temperatures.
Material and Methods: Possible antimicrobial effects of ropivacaine (1% and 0.125%), levobupivacaine (0.75% and 0.125%) and bupivacaine (0.5% and 0.125%) on S.aureus ATCC 6538, S.epidermidis, E.coli and C.albicans ATCC 10231, which were isolated from patients in the microbiology laboratory were investigated at 240C and 37C.
Results: All three LA drugs showed antifungal activity at 37°C and 24°C in 3rd and 12th hours. Levobupivacaine’s (0.75%) antibacterial effect was observed at the 12th hour. Ropivacaine (1%) had relatively more antibacterial effect at the 3rd and 12th hour at 37°C rather than 24°C whereas bupivacaine had less.
Conclusion: Potential clinical implications of antibacterial or antifungal effects of local anesthetics might be promising.

References

  • Borgeat A. Non anesthetic action of local anesthetics. Periodicum Biologorum. 2013; 115(2): 113-117.
  • Goodman EJ, Jacobs MR, Bajaksouzian S, Windau AR, Dagirmanjian JP. Clinically significant concentrations of local anesthetics inhibit Staphylococcus aureus in vitro. Int J Obstet Anesth. 2002;11(2):95-99.
  • Kaya K, Rota S, Doğan B, Kökten G, Günaydın B, Bozdayı G. Comparison of the Antibacterial Effects of Two Local Anesthetics: Lidocaine and Articaine. Turk J Med Sci. 2007; 37(1): 7-10.
  • Johnson SM, Saint John BE, Dine AP. Local anesthetics as antimicrobial agents: a review. Surg Infect (Larchmt). 2008;9(2):205-213. doi:10.1089/sur.2007.036
  • Adlerr DMT, Domborg P, Verwilghen DR.Antimicrobial activity of bupivacaine, lidocaine and mepivacaien against equine pathogens: An investigation of 40 bacterial isolates. Veterinery Journal. 2017; 23: 27-31.
  • Sakuragi T, Ishino H, Dan K. Bactericidal activity of preservative-free bupivacaine on microorganisms in the human skin flora. Acta Anaesthesiol Scand. 1998;42(9):1096-1099. doi:10.1111/j.1399-6576.1998.tb05383.x.
  • Hodson M, Gajraj R, Scott NB. A comparison of the antibacterial activity of levobupivacaine vs. bupivacaine: an in vitro study with bacteria implicated in epidural infection. Anaesthesia. 1999;54(7):699-702. doi:10.1046/j.1365-2044.1999.00742.x.
  • Guillier M, Boselli E, Bouvet L, et al. Levobupivacaine hydrochloride and sufentanil have no antimicrobial effect at 25 degrees C in vitro. Eur J Anaesthesiol. 2007;24(7):634-639. doi:10.1017/S0265021507000026.
  • Kampe S, Poetter C, Buzello S, et al. Ropivacaine 0.1% with sufentanil 1 microg/mL inhibits in vitro growth of Pseudomonas aeruginosa and does not promote multiplication of Staphylococcus aureus. Anesth Analg. 2003;97(2):409-411. doi:10.1213/01.ANE.0000067404.84486.7A.
  • Bátai I, Kerényi M, Falvai J, Szabó G. Bacterial growth in ropivacaine hydrochloride. Anesth Analg. 2002;94(3):729-731. doi:10.1097/00000539-200203000-00046.
  • Pere P, Lindgren L, Vaara M. Poor antibacterial effect of ropivacaine: comparison with bupivacaine. Anesthesiology. 1999;91(3):884-886. doi:10.1097/00000542-199909000-00047.
  • Tamanai-Shaccori Z, Shaccori V, Vo Van JM, Robert JC, Bonnaure-Mallet M. Sufentanil modifies the antibacterial activity of bupivacaine and ropivacaine. Reg Anesth Pain Med 2004; 51: 911-4.
  • Coghlan MW, Davies MJ, Hoyt C, Joyce L, Kilner R, Waters MJ. Antibacterial activity of epidural infusions. Anaesth Intensive Care. 2009;37(1):66-69.
  • Aydin ON, Eyigor M, Aydin N. Antimicrobial activity of ropivacaine and other local anaesthetics. Eur J Anaesthesiol. 2001;18(10):687-694. doi:10.1046/j.1365-2346.2001.00900.x.
  • Kose AA, Karabağlı Y, Kiremitci A, Kocman E, Cetin C. Do local anesthetics have antibacterial effect on staphyloccus aureus under in vivo conditions? An experiemental study. Dermatol Surg. 2010; 36: 848-52.
  • Rodrigues AA, Pina-Vaz C, Mårdh PA, Martinez-de-Oliveira J, Freitas-da-Fonseca A. Inhibition of germ tube formation by Candida albicans by local anesthetics: an effect related to ionic channel blockade. Curr Microbiol. 2000;40(3):145-148. doi:10.1007/s002849910030.
  • Pina-Vaz C, Rodrigues AG, Sansonetty F, Martinez-De-Oliveira J, Fonseca AF, Mårdh PA. Antifungal activity of local anesthetics against Candida species. Infect Dis Obstet Gynecol. 2000;8(3-4):124-137. doi:10.1155/S1064744900000168.

Ropivakain, levobupivakain ve bupivakainin farklı sıcaklıklardaki antimikrobiyal etkileri

Year 2024, Volume: 15 Issue: 3, 400 - 405, 30.09.2024
https://doi.org/10.18663/tjcl.1523812

Abstract

Amaç: Lokal anestezik (LA) ilaçların, daha önce yapılan çeşitli çalışmalarla olası bir antimikrobiyal aktivitesi gösterilmiştir. Bu çalışmanın amacı, rejyonel anestezi uygulamalarında yaygın olarak kullanılan ropivakain, levobupivakain ve bupivakain gibi ticari olarak mevcut LA ilaçlarının farklı sıcaklıklarda S.aureus ATCC 6538, S. epidermidis, E.coli ve Candida albicans üzerindeki antimikrobiyal (antibakteriyel veya antifungal) aktivitesini değerlendirmektir.
Gereç ve Yöntemler: Mikrobiyoloji laboratuvarında ropivakain (%1 ve %0,125), levobupivakain (%0,75 ve %0,125) ve bupivakainin (%0,5 ve %0,125) hastalardan izole edilen S.aureus ATCC 6538, S.epidermidis, E.coli ve C.albicans ATCC 10231 üzerindeki olası antimikrobiyal etkileri 240C ve 370C’de araştırıldı.
Bulgular: Her üç lokal anestezik ilaç da, 37°C ve 24°C'de 3. ve 12. saatlerde antifungal aktivite göstermiştir. Levobupivakainin (%0,75) antibakteriyel etkisi 12. saatte gözlendi. Ropivakain (%1) 37°C'de 3. ve 12. saatlerde 24°C'ye göre nispeten daha fazla antibakteriyel etkiye sahipken, bupivakain daha az antibakteriyel etkiye sahipti.
Sonuçlar: Lokal anesteziklerin antibakteriyel veya antifungal etkilerinin potansiyel klinik sonuçları umut verici olabilir.

References

  • Borgeat A. Non anesthetic action of local anesthetics. Periodicum Biologorum. 2013; 115(2): 113-117.
  • Goodman EJ, Jacobs MR, Bajaksouzian S, Windau AR, Dagirmanjian JP. Clinically significant concentrations of local anesthetics inhibit Staphylococcus aureus in vitro. Int J Obstet Anesth. 2002;11(2):95-99.
  • Kaya K, Rota S, Doğan B, Kökten G, Günaydın B, Bozdayı G. Comparison of the Antibacterial Effects of Two Local Anesthetics: Lidocaine and Articaine. Turk J Med Sci. 2007; 37(1): 7-10.
  • Johnson SM, Saint John BE, Dine AP. Local anesthetics as antimicrobial agents: a review. Surg Infect (Larchmt). 2008;9(2):205-213. doi:10.1089/sur.2007.036
  • Adlerr DMT, Domborg P, Verwilghen DR.Antimicrobial activity of bupivacaine, lidocaine and mepivacaien against equine pathogens: An investigation of 40 bacterial isolates. Veterinery Journal. 2017; 23: 27-31.
  • Sakuragi T, Ishino H, Dan K. Bactericidal activity of preservative-free bupivacaine on microorganisms in the human skin flora. Acta Anaesthesiol Scand. 1998;42(9):1096-1099. doi:10.1111/j.1399-6576.1998.tb05383.x.
  • Hodson M, Gajraj R, Scott NB. A comparison of the antibacterial activity of levobupivacaine vs. bupivacaine: an in vitro study with bacteria implicated in epidural infection. Anaesthesia. 1999;54(7):699-702. doi:10.1046/j.1365-2044.1999.00742.x.
  • Guillier M, Boselli E, Bouvet L, et al. Levobupivacaine hydrochloride and sufentanil have no antimicrobial effect at 25 degrees C in vitro. Eur J Anaesthesiol. 2007;24(7):634-639. doi:10.1017/S0265021507000026.
  • Kampe S, Poetter C, Buzello S, et al. Ropivacaine 0.1% with sufentanil 1 microg/mL inhibits in vitro growth of Pseudomonas aeruginosa and does not promote multiplication of Staphylococcus aureus. Anesth Analg. 2003;97(2):409-411. doi:10.1213/01.ANE.0000067404.84486.7A.
  • Bátai I, Kerényi M, Falvai J, Szabó G. Bacterial growth in ropivacaine hydrochloride. Anesth Analg. 2002;94(3):729-731. doi:10.1097/00000539-200203000-00046.
  • Pere P, Lindgren L, Vaara M. Poor antibacterial effect of ropivacaine: comparison with bupivacaine. Anesthesiology. 1999;91(3):884-886. doi:10.1097/00000542-199909000-00047.
  • Tamanai-Shaccori Z, Shaccori V, Vo Van JM, Robert JC, Bonnaure-Mallet M. Sufentanil modifies the antibacterial activity of bupivacaine and ropivacaine. Reg Anesth Pain Med 2004; 51: 911-4.
  • Coghlan MW, Davies MJ, Hoyt C, Joyce L, Kilner R, Waters MJ. Antibacterial activity of epidural infusions. Anaesth Intensive Care. 2009;37(1):66-69.
  • Aydin ON, Eyigor M, Aydin N. Antimicrobial activity of ropivacaine and other local anaesthetics. Eur J Anaesthesiol. 2001;18(10):687-694. doi:10.1046/j.1365-2346.2001.00900.x.
  • Kose AA, Karabağlı Y, Kiremitci A, Kocman E, Cetin C. Do local anesthetics have antibacterial effect on staphyloccus aureus under in vivo conditions? An experiemental study. Dermatol Surg. 2010; 36: 848-52.
  • Rodrigues AA, Pina-Vaz C, Mårdh PA, Martinez-de-Oliveira J, Freitas-da-Fonseca A. Inhibition of germ tube formation by Candida albicans by local anesthetics: an effect related to ionic channel blockade. Curr Microbiol. 2000;40(3):145-148. doi:10.1007/s002849910030.
  • Pina-Vaz C, Rodrigues AG, Sansonetty F, Martinez-De-Oliveira J, Fonseca AF, Mårdh PA. Antifungal activity of local anesthetics against Candida species. Infect Dis Obstet Gynecol. 2000;8(3-4):124-137. doi:10.1155/S1064744900000168.
There are 17 citations in total.

Details

Primary Language English
Subjects Anaesthesiology, Clinical Microbiology
Journal Section Research Article
Authors

Fatmanur Duruk Erkent 0000-0002-9161-5013

Berrin Günaydın 0000-0002-0422-5536

Merve Erdoğan 0000-0003-3845-9100

Ayşe Kalkancı 0000-0003-0961-7325

Publication Date September 30, 2024
Submission Date July 28, 2024
Acceptance Date August 21, 2024
Published in Issue Year 2024 Volume: 15 Issue: 3

Cite

APA Duruk Erkent, F., Günaydın, B., Erdoğan, M., Kalkancı, A. (2024). Antimicrobial effects of ropivacaine, levobupivacaine and bupivacaine at different temperatures. Turkish Journal of Clinics and Laboratory, 15(3), 400-405. https://doi.org/10.18663/tjcl.1523812
AMA Duruk Erkent F, Günaydın B, Erdoğan M, Kalkancı A. Antimicrobial effects of ropivacaine, levobupivacaine and bupivacaine at different temperatures. TJCL. September 2024;15(3):400-405. doi:10.18663/tjcl.1523812
Chicago Duruk Erkent, Fatmanur, Berrin Günaydın, Merve Erdoğan, and Ayşe Kalkancı. “Antimicrobial Effects of Ropivacaine, Levobupivacaine and Bupivacaine at Different Temperatures”. Turkish Journal of Clinics and Laboratory 15, no. 3 (September 2024): 400-405. https://doi.org/10.18663/tjcl.1523812.
EndNote Duruk Erkent F, Günaydın B, Erdoğan M, Kalkancı A (September 1, 2024) Antimicrobial effects of ropivacaine, levobupivacaine and bupivacaine at different temperatures. Turkish Journal of Clinics and Laboratory 15 3 400–405.
IEEE F. Duruk Erkent, B. Günaydın, M. Erdoğan, and A. Kalkancı, “Antimicrobial effects of ropivacaine, levobupivacaine and bupivacaine at different temperatures”, TJCL, vol. 15, no. 3, pp. 400–405, 2024, doi: 10.18663/tjcl.1523812.
ISNAD Duruk Erkent, Fatmanur et al. “Antimicrobial Effects of Ropivacaine, Levobupivacaine and Bupivacaine at Different Temperatures”. Turkish Journal of Clinics and Laboratory 15/3 (September 2024), 400-405. https://doi.org/10.18663/tjcl.1523812.
JAMA Duruk Erkent F, Günaydın B, Erdoğan M, Kalkancı A. Antimicrobial effects of ropivacaine, levobupivacaine and bupivacaine at different temperatures. TJCL. 2024;15:400–405.
MLA Duruk Erkent, Fatmanur et al. “Antimicrobial Effects of Ropivacaine, Levobupivacaine and Bupivacaine at Different Temperatures”. Turkish Journal of Clinics and Laboratory, vol. 15, no. 3, 2024, pp. 400-5, doi:10.18663/tjcl.1523812.
Vancouver Duruk Erkent F, Günaydın B, Erdoğan M, Kalkancı A. Antimicrobial effects of ropivacaine, levobupivacaine and bupivacaine at different temperatures. TJCL. 2024;15(3):400-5.


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