Dağ kekiği (Origanum syriacum L. var. bevanii (Holmes) Ietswaart)’nin in vivo ve in vitro koşullarda yetiştirilen bitkilerinin ve kalluslarının uçucu yağ bileşenlerinin bir kıyaslaması

Year 2023, Volume: 24 Issue: 2, 45 - 55, 28.06.2023

A. Haluk Türker

https://doi.org/10.18182/tjf.1281648

Abstract

Bu araştırma, Origanum syriacum var. bevanii taksonunun donör olarak kullanılan in vivo bitkileri ile in vitro koşullarda yetiştirilen rejenerant bitkileri ve kalluslarının uçucu yağ bileşenlerinin belirlenmesi ve kıyaslanması amacıyla yürütülmüştür. Doğal ortamından alınan çeliklerle yetiştirilen donör bitkiler ve bunlardan alınan çeşitli eksplantlarla farklı konsantrasyonlarda bitki büyüme düzenleyicileri ilave edilen in vitro ortamda yetiştirilen rejenerant bitkiler ve kallusların uçucu yağ bileşenleri GC/MS (Gaz Kromatografisi - Kütle Spektrometrisi) cihazı ile belirlenmiştir. Araştırma sonucunda, donör ve rejenerant bitkiler ve kallusların uçucu yağ bileşenlerinin birbirinden oldukça farklı olduğu belirlenmiştir. Ana bileşenler olarak, donör bitkilerde %36,28 Carvacrol ve %19,76 ortho-Cymene tespit edilmişken rejenerant bitkilerde %26,49 Carbamic Acid ve %19,67 Carvacrol tespit edilmiştir. Tamamen farklı olarak, kalluslardaki ana bileşenler %64,82 Ethanol, %57,23 1,2-Propanediol, %55,11 L(+)-Lactic acid, %53,53 Methyltartronic acid, %52,16 4-Penten-2-ol ve %38,47 2-Methoxycinnamic acid olarak tespit edilmiştir. Basit fenolik bileşiklerin oluşumunda başlangıç bileşenlerinden olan 2-Methoxycinnamic acid, sap boğumu eksplantından gelişen kallusta yaprak eksplantından gelişen diğer kalluslara göre en yüksek oranda bulunmakta (%38,4) ve ana bileşeni oluşturmaktadır. Dolayısıyla analiz sonuçlarına göre sap boğumu eksplantından gelişen kallusun sekonder metabolitlerin oluşumunda ümitvar sonuçlar verdiği tespit edilmiştir.

Keywords

Origanum syriacum var. bevanii, Rejenerant bitki, Kallus, Uçucu yağ bileşenleri

Supporting Institution

Doğu Akdeniz Ormancılık Araştırma Enstitüsü Müdürlüğü

Project Number

20.7705

Thanks

Bu makale; Orman Genel Müdürlüğü, Doğu Akdeniz Ormancılık Araştırma Enstitüsü Müdürlüğünce yürütülen 20.7705 proje numaralı ve “Biberiye (Rosmarinus officinalis L.) ve Dağ kekiğinin (Origanum syriacum L. var. bevanii (Holmes) Ietswaart) in vitro rejenerasyon olanaklarının ve uçucu yağ bileşenlerinin araştırılması” isimli araştırma projesi kapsamında hazırlanmıştır. Uçucu yağ analizleri için Prof. Dr. Ebru KAFKAS’a ve katkılarından dolayı Prof. Dr. Rüştü HATİPOĞLU’na teşekkür ederim.

A comparison of essential oil components of plants and calli grown in vivo and in vitro conditions of bible hyssop (Origanum syriacum L. var. bevanii (Holmes) Ietswaart)

Abstract

This research was carried out to determine and compare the essential oil components of regenerated plants and calli grown under in vitro conditions with in vivo plants of Origanum syriacum var. bevanii taxon used as donor. The essential oil components of the donor plants grown with cuttings taken from the native environment and regenerant plants and calli grown from the various explants in vitro supplemented with different concentrations of the plant growth regulators were determined by Gas Chromatography-Mass Spectrometry (GC-MS). In result of the research, it was determined that the essential oil components of donor and regenerant plants and calli were quite different from each other. It was determined that was Carbamic Acid (26.49%) and Carvacrol (19.67%) in essential oil from the regenerant plants while was Carvacrol (36.28%) and ortho-Cymene (19.76%) in essential oil from the donor plants as major components. It was determined that was 64.82% Ethanol, 57.23% 1,2-Propanediol, 55.11% L(+)-Lactic acid, 53.53% Methyltartronic acid, 52.16% 4-Penten-2-ol ve 38.47% 2-Methoxycinnamic acid in essential oil from the callus as major components disparately. 2-Methoxycinnamic acid, which is one of the initial components in the formation of simple phenolic compounds, was found at the highest rate (38.4%) in the callus developed from the stem node explant, compared to the other callus developed from the leaf explant, and constitutes the main component. Therefore, according to the results of the analysis, it was determined that the callus developed from the stem node explant gave promising results in the formation of secondary metabolites.

Keywords

Bible hyssop, Regenerant plant, Callus, Essential oil components

Project Number

20.7705

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