GENETIC ANALYSIS OF POWDERY MILDEW RESISTANCE GENE USING SSR MARKERS IN COMMON WHEAT ORIGINATED FROM WILD EMMER (Triticum dicoccoides Thell)

Year 2016, , 10 - 15, 01.06.2016

Mamoudou Saıdou Changyou Wang Md. Ashraful Alam Chunhuan Chen Wanquan Jı

https://doi.org/10.17557/tjfc.83589

Cited By: 2

Abstract

Common wheat line N0324, derived from the cross wild emmer (Triticum dicoccoides Thell) accession 5055 and
Shaan 253, provides effective protection to powdery mildew. Analysis of 205 individuals in the segregating F2
population generated by crossing N0324 with the susceptible cultivar Shaanyou 225 revealed that resistance in
N0324 line was controlled by a single recessive gene designated temporarily as Pm5055. Bulked segregant
analysis (BSA) and simple sequence repeats (SSRs) were used to characterize the powdery mildew resistance
gene Pm5055. Four SSR markers such as Xwmc441, Xbarc7, Xbarc13 and Xbarc55 were linked to the gene with
genetic distances of 10.6, 23, 23 and 23.9 cM, respectively. Chinese Spring nulli-tetrasomic and ditelosomic lines
were used to assign the chromosomal locations of the linked markers. The results suggested that this gene might
be located on chromosome 2B. Pm5055 can be used to diversify powdery mildew resistance sources in future
wheat breeding programs.

Keywords

Blumeria graminis f. sp. tritici, molecular markers, Triticum dicoccoides, wheat breeding

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