Kiraz Domatesinde (Solanum lycopersicum L. var. cerasiforme) in vitro ve ex vitro koşullardaki mutasyon dozunun belirlenmesi

Abstract

Bu çalışmada, kiraz domatesi (Solanum lycopersicum L. var. cerasiforme) tohumlarının farklı çimlenme koşullarında (in vitro ve ex vitro) gama ışınına maruz bırakılması sonucu oluşan biyolojik reaksiyonlar değerlendirilerek etkili mutasyon dozu (EMD₅₀) belirlenmiştir. İn vitro denemede, tohumlar 0–80 Gy aralığında gama ışınlarıyla ışınlanmış ve herhangi bir bitki büyüme düzenleyicisi içermeyen Murashige ve Skoog (MS) besiyerinde çimlendirilmiştir. Ex vitro koşullarda ise tohumlar 0–600 Gy arasında değişen dozlarda ışınlanarak toprak karışımıyla doldurulmuş saksılara ekilmiştir. Işınlamadan sonraki ilk 10 günde tohum çimlenmesi ve toplamda 30 günlük inkübasyon süresinin sonunda fidelerde sürgün uzunluğu, yaş ve kuru ağırlık gibi parametreler ölçülmüştür. Yapılan regresyon analizleri sonucunda, in vitro koşullarda EMD₅₀ değeri 39.08 Gy, ex vitro koşullarda ise 608.6 Gy olarak hesaplanmıştır. Elde edilen bulgular, in vitro ve in vivo koşullarda yapılan ışınlama dozunun ışınlama koşullarına bağlı olarak değiştiğini göstermiştir. Bu sonuçlar, mutasyon ıslahı çalışmalarında uygun ışınlama dozunun seçilmesinde eksplantın kültüre alınacağı ortama bağlı olarak dikkate alınması gerektiğinin önemini ortaya koymaktadır.

Keywords

• Etkili mutasyon dozu (EMD50)
• in vitro
• ex vitro
• Kiraz domatesi
• solanum lycopersicum L. var. cerasiforme

Mutation dose determination of cherry tomato (Solanum lycopersicum L. cerasiforme) under in vitro and ex vitro conditionsions

Abstract

In this study, biological responses resulting from gamma irradiation of cherry tomato (Solanum lycopersicum L. var. cerasiforme) seeds under different germination conditions (in vitro and ex vitro) were evaluated, and the effective mutation dose (EMD₅₀) was determined. In the in vitro experiment, seeds were irradiated with gamma rays at doses ranging from 0 to 80 Gy and germinated on Murashige and Skoog (MS) medium without any plant growth regulators. In the ex vitro conditions, seeds were exposed to doses between 0 and 600 Gy and sown in pots filled with a soil mixture. Seed germination was monitored during the first 10 days post-irradiation, and parameters such as shoot length, fresh weight, and dry weight of seedlings were measured at the end of a 30-day incubation period. Regression analyses revealed an EMD₅₀ value of 39.08 Gy under in vitro conditions and 608.6 Gy under ex vitro conditions. The findings demonstrate that the effective irradiation dose varies depending on the irradiation conditions (in vitro vs. ex vitro). These results emphasize the importance of considering the culture conditions when selecting the appropriate irradiation dose in mutation breeding programs in cherry tomato.

Keywords

• Effective mutation dose (EMD50)
• in vitro
• ex vitro
• Cherry Tomato
• Solanum lycopersicum L. cerasiforme

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