Determination of antioxidant activity of Medicago sativa L. flowers by biosensor and quantitative analysis of bioactive compounds by LC-MS/MS
Abstract
Natural products play a vital role in various aspects of human life, particularly in health, agriculture, and industry. These are substances derived from plants, animals, microorganisms, and minerals, and they have been used for centuries. Natural products have historically served as the foundation for many modern medicines. Over 50% of current pharmaceuticals are derived from or inspired by natural compounds. Plants are a rich and vital source of natural antioxidants that protect the body from oxidative stress caused by free radicals. The antioxidant effect of Medicago sativa L. flowers (MSF) was determined by sensory. Moreover, quantitative analysis of phenolics in MSF was presented. MSF scavenged DPPH radical by 41.71%. This value indicates that the plant exhibits a good antioxidant effect. Total phenolic content of MSF was determined to be 92.18 mg GA/g extract. MSF contained kaempferol (88.99), coumarin (24.42), and Kaempferol-3-glucoside (20.39 µg/g extract). Consequently, this plant's flowers include the pharmaceutically critical natural compounds for food and pharmaceutical industries.
Keywords
Medicago sativa , Sensory , Quantitative analysis , Antioxidant activity
References
- Fracchiolla, N.S., Artuso, S. & Cortelezzi, A. (2013). Biosensors in clinical practice: focus on oncohematology. Sensors, 13(5), 6423-6447.
- Fetz, V., Knauer, S.K., Bier, C., Von Kries, J.P. & Stauber, R.H. (2009). Translocation biosensors–cellular system integrators to dissect CRM1-dependent nuclear export by chemicogenomics. Sensors, 9(7), 5423-5445.
- Knauer, S.K., Moodt, S., Berg, T., Liebel, U., Pepperkok, R. & Stauber, R.H. (2005). Translocation biosensors to study signal‐specific nucleo‐cytoplasmic transport, protease activity and protein–protein interactions. Traffic, 6(7), 594-606.
- Topçu, G., Erenler, R., Çakmak, O., Johansson, C.B., Çelik, C., Chai, H.-B. & Pezzuto, J.M. (1999). Diterpenes from the berries of Juniperus excelsa. Phytochemistry, 50(7), 1195-1199.
- Elmastas, M., Ozturk, L., Gokce, I., Erenler, R. & Aboul‐Enein, H.Y. (2004). Determination of antioxidant activity of marshmallow flower (Althaea officinalis L.). Analytical Letters, 37(9), 1859-1869.
- Demirtas, I., Erenler, R., Elmastas, M. & Goktasoglu, A. (2013). Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction. Food Chemistry, 136(1), 34-40.
- Genç, N., Elmastaş, M., Telci, İ. & Erenler, R. (2020). Quantitative analysis of phenolic compounds of commercial basil cultivars (Ocimum basilicum L.) by LC-TOF-MS and their antioxidant effects. International Journal of Chemistry and Technology, 4(2), 179-184.
- Erenler, R., Geçer, E.N., Alma, M.H. & Demirtas, İ. (2022). Quantitative Analysis of Bioactive Compounds by High‑Performance Liquid Chromatography in Origanum bilgeri. Journal of Integrative and Anatolian Medicine, 4(1), 15-20.
- Yaman, C., Önlü, Ş., Ahmed, H. & Erenler, R. (2022). Comparison of phytochemicals and antioxidant capacity of Hypericum perforatum; wild plant parts and in vitro samples. Journal of Animal & Plant Sciences, 32(2), 596-603.
- Erenler, R., Gecer, E.N., Genc, N. & Yanar, D. (2021). Antioxidant activity of silver nanoparticles synthesized from Tagetes erecta L. leaves. International Journal of Chemistry and Technology, 5(2), 141-146.