Determination of TYLCV-Resistant Cherry and Cocktail Tomato Cultivars by Molecular Markers

Abstract

ABSTRACT In this study, resistant cultivars to Tomato yellow leaf curly virus (TYLCV) of different cherry and cocktail tomato cultivars were determined using molecular DNA markers. For this purpose, resistance to Tomato Yellow Leaf Curly Virus (TYLCV) of a total of 409 different cherry and cocktail tomato cultivars was determined by polymerase chain reaction (PCR) using the primer Ty3P6-25. As a result of the assays, 291 cherry and cocktail tomato cultivars were found to be susceptible (rr), 66 cultivars were heterozygous resistant (Rr), and 45 cultivars were homozygous resistant (RR) to TYLCV. In addition, no molecular markers were detected in 7 cherry and cocktail tomato cultivars. It was found that the molecular DNA marker used is useful in determining resistance responses to TYLCV in cherry tomato and cocktail tomato and can provide reproducible and reliable results in a short time.

Keywords

• Cherry and Coctail tomatoes
• Molecular marker
• Tomato Yellow Leaf Curl Virus

TYLCV'ye Dayanıklı Kiraz ve Kokteyl Domates Çeşitlerinin Moleküler Markörler ile Belirlenmesi

Abstract

Bu çalışmada, farklı kiraz ve kokteyl domates çeşitlerinin Domates sarı yaprak kıvırcık virüsüne (TYLCV) karşı dayanıklılıkları moleküler DNA markörleri kullanılarak belirlenmiştir. Bu amaçla, Ty3P6-25 primeri kullanılarak polimeraz zincir reaksiyonu (PCR) ile toplam 409 farklı kiraz ve kokteyl domates çeşidinin Tomato Yellow Leaf Curly Virus'a (TYLCV) dayanıklılığı belirlenmiştir. Testler sonucunda, 291 kiraz ve kokteyl domates çeşidinin TYLCV'ye duyarlı (rr), 66 çeşidin heterozigot dayanıklı (Rr) ve 45 çeşidin homozigot dayanıklı (RR) olduğu tespit edilmiştir. Ayrıca, 7 kiraz ve kokteyl domates çeşidinde herhangi bir moleküler belirteç tespit edilmemiştir. Kullanılan moleküler DNA markörünün kiraz domatesi ve kokteyl domatesinde TYLCV'ye karşı direnç yanıtlarını belirlemede yararlı olduğu ve kısa sürede tekrarlanabilir ve güvenilir sonuçlar sağlayabileceği bulunmuştur.

Keywords

• Domates Sarı Yaprak Kıvrılma Virüsü
• Kiraz ve kokteyl domatesleri
• Moleküler markör

Thanks

This work was supported by Istanbul Tarım AŞ.

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