Antimicrobial and Antioxidant Activities of Different Extracts of Helichrysum arenarium subsp. (L.) Moench aucheri

Year 2023, Volume: 18 Issue: 2, 345 - 351, 01.09.2023

Ayşe Eren Şule İnci Kochar Kh. Saleh Sevda Kırbağ Kemal Güven

https://doi.org/10.55525/tjst.1260055

Abstract

Helichrysum arenarium (L.) Moench subsp. aucheri is a herbaceous perennial herb belonging to the Asteraceae. This plant has biological activities such as antibacterial, antiviral, anti-inflammatory, antifungal, antiproliferative, antioxidant, and antiradical. In this study, antimicrobial and antioxidant activities of methanol and ethanol extracts of aerial parts of H. arenarium subsp. aucheri were investigated. To determine the antimicrobial activity pathogenic microorganisms Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus megaterium, Candida glabrata, Candida albicans and Trichophyton sp. Antioxidant activity was determined with total antioxidant value (TAS), total oxidant value (TOS) and 2.2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging capacity. In the results obtained, it was determined that the methanol extract had an antimicrobial effect (9.3 mm) only against C. albicans. It was found that the ethanol extract showed antimicrobial activity at different rates (8.8-20.4 mm) against S. aureus, B. megaterium, C. glabrata, C. albicans and Trichophyton sp. The TAS value of the methanol extract was 3.00 mmol, and the TAS value of the ethanol extract was 3.15 mmol. The TOS value of the methanol extract of the same species was calculated as 6.81 μmol, and the TOS value of the ethanol extract was calculated as 12.64 μmol. The DPPH radical scavenging effects of extracts of goldengrass was found to increase depend on concentrations.

Keywords

Antimicrobial effect, antioxidant effect, goldengrass, Helichrysum arenarium

Helichrysum arenarium subsp. (L.) Moench aucheri’nin Farklı Ekstraktlarının Antimikrobiyal ve Antioksidan Aktivitesi

Abstract

Helichrysum arenarium (L.) Moench subsp. aucheri, Asteraceae ait otsu çok yıllık bir bitkidir. Bu bitki, antibakteriyel, antiviral, antiinflamatuar, antifungal, antiproliferatif, antioksidan, antiradikal gibi biyolojik aktivitelere sahiptir. Bu çalışmada, H. arenarium subsp. aucheri’nin toprak üstü kısımlarının metanol ve etanol ekstraktlarının antimikrobiyal ve atioksidan aktiviteleri araştırılmıştır. Antimikrobiyal aktivitenin belirlenebilmesi için Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus megaterium, Candida glabrata, Candida albicans, Trichophyton sp. patojenik mikroorganizmalar kullanılmıştır. Antioksidan aktivite toplam antioksidan değeri (TAS), toplam oksidan değeri (TOS) ve 2.2-diphenyl-1-picrilhydrazyl (DPPH) radikal süpürme kapasitesi ile belirlenmiştir. Elde edilen sonuçlarda metanol ekstresinin sadece C. albicans’a karşı antimikrobiyal etkisinin (9.3 mm) olduğu tespit edilmiştir. Etanol ekstresinin ise S. aureus, B. megaterium, C. glabrata, C. albicans, ve Trichophyton sp.’ye karşı farklı oranlarda antimikrobiyal etki (8.8-20.4 mm) gösterdiği bulunmuştur. Metanol ekstresinin TAS değeri 3.00 mmol, etanol ekstresinin TAS değeri 3.15 mmol olarak tespit edilmiştir. Aynı türün metanol ekstresinin TOS değeri 6.81 μmol, etanol ekstresinin TOS değeri 12.64 μmol olarak hesaplanmıştır. Altın otun’un ekstrelerinin DPPH radikalini süpürücü etkilerinin artan konsantrasyonlara bağlı olarak arttığı belirlenmiştir.

Keywords

Antimikrobiyal, antioksidan, altınotu, Helichrysum arenarium

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