Molecular examination of ESβL, Amp-C, and carbapenemase genes in Klebsiella pneumoniae and Escherichia coli isolates from raw milk
Abstract
Objective: In this study, the detection of ESβL, Amp-c, and carbapenemase genes was aimed at K. pneumoniae and E. coli isolates obtained from raw milk collected from a total of 150 healthy cows (120 samples) located in the Ankara Region and raw milk samples (30 samples) available in markets. Materials and Methods: In this study, a total of 150 raw milk samples (120) from healthy cows and 30 from different companies were offered for sale in markets) from 9 different family businesses located in the Ankara Region were used. Results: As a result of conventional isolation from raw milk samples, 4 K. pneumoniae and 1 E. coli were identified by traditional methods and a rapid identification kit (BBL Crystal). When examined in terms of ESβL activity, CTX-M1, SHV, TEM-1, CTX-M9, OXA-1 genes; in terms of Amp-C activity, FOX gene; and terms of carbapenemase activity, OXA-48, KPC, VIM and NDM genes were detected by the classical PCR. Conclusion: A higher rate of ESβL, Amp-C ve carbapenemase genes were detected in E. coli and K. pneumonia isolates which are isolated and identified from raw milk in the Ankara Region than in many other studies. For this reason, it is considered that milk obtained from small family businesses that feed cows and raw milk sold in markets should be followed in terms of antibiotic resistance and that people who consume these products are at risk in terms of antibiotic resistance.
Keywords
Amp-C carbapenemase Extended Spectrum Beta-Lactamase Escherichia coli Klebsiella pneumonia.
Ethical Statement
Etik Beyana gerek bulunmamaktadır.
Supporting Institution
The authors declared that there is no conflict of interest.
Project Number
2022/069
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Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Microbiology |
| Journal Section | Research Article |
| Authors | |
| Project Number | 2022/069 |
| Submission Date | October 16, 2025 |
| Acceptance Date | March 11, 2026 |
| Publication Date | March 30, 2026 |
| DOI | https://doi.org/10.47748/tjvr.1803810 |
| IZ | https://izlik.org/JA82AR95LK |
| Published in Issue | Year 2026 Volume: 10 Issue: 1 |
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Prof. Dr. İsmail Hakkı Ekin, 1971 Yılında Siirt'te doğdu. İlk ve ortaöğrenimini Van'da tamamladı. 1989 yılında Van Teknik Lisesi Motor Bölümünü birincilikle bitirdi. 1990 yılında girdiği Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesinden 1995 yılında mezun oldu. Aynı yıl kısa dönem askerlik görevini yerine getirerek, Temmuz 1996’da terhis oldu. 1997’de Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi’nde Araştırma Görevliliğine atandı. Van Yüzüncü Yıl Üniversitesi Sağlık Bilimleri Enstitüsü Veteriner Mikrobiyoloji Anabilim Dalında başladığı Yüksek Lisans öğrenimini 1998 yılında, Doktora öğrenimini ise 2004 yılında bitirerek Bilim Doktoru (PhD) unvanını aldı. Dr. Araştırma görevlisi olarak 2 yıl süreyle görev yaptıktan sonra Şubat 2006’da Dr. Öğretim Üyesi (Yrd. Doç. Dr.) kadrosuna atandı. Van Yüzüncü Yıl Üniversitesi Sağlık Bilimleri Enstitüsü Besin Hijyeni ve Teknolojisi Anabilim Dalındaki ikinci Doktora eğitimini de 2022 yılında bitirerek ikinci Bilim Doktoru (PhD) unvanını aldı. 2013 yılında Doçentlik unvanını alan Ekin, 2018 yılında Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Mikrobiyoloji Anabilim Dalında Profesörlük kadrosuna atandı. Halen bu birimde Anabilim Dalı Başkanı olarak görevini sürdürmekte olup evli ve üç çocuk babasıdır.
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