Asteraceae Ailesine Ait Farklı Türlerin Antibakteriyel Aktivitesinin Belirlenmesi

Abstract

Son yıllarda alternatif tıp uygulamalarında bitkilerin kullanımı artmıştır. Sentetik kimyasalların görülen zararlı etkilerinden dolayı tıbbi bitkilerden elde edilen özütlerin antioksidan, antibakteriyel ve antifungal özellikleri araştırılmaktadır. Bu çalışmada, Amasya ilinde farklı ekolojik şartlarda yayılış gösteren bazı bitki türlerinin antibakteriyel aktiviteleri araştırılmıştır. Asteraceae ailesine ait Anthemis tinctorial L., Matricaria chamomilla L. ve Achillea biebersteinii Afan türlerinin metanolik özütleri sokslet cihazı yardımıyla elde edilmiştir. Elde edilen özütlerin antibakteriyal aktivitesi disk difüzon ve mikrodilüsyon yöntemi ile belirlenmiştir. Antibakteriyel aktivite için Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa ve Salmonella enteritidis standart suşları kullanılmıştır. Bu çalışmada, bitki özütleri kontrol olarak kullanılan antibiyotik grubuyla karşılaştırıldığında antibakteriyel etkiye sahip olduğu gözlemlenmiştir. Disk difüzyon yönteminin sonuçlarına göre, en yüksek antibakteriyel etki sırasıyla A. tinctorial, M. chamomilla ve A. biebersteinii olarak belirlenmiştir. Mikrodilüsyon yönteminde bitki özütlerinin konsantrasyon aralığı 6,25 - 50 mg/mL olacak şekilde ayarlanmıştır. Sonuç olarak çalışmada kullanılan bitki özütlerinin alternatif tedavi amacıyla kullanılabileceği ve daha sonra yapılacak antibakteriyel çalışmalara yön göstereceği düşünülmektedir.

Keywords

• Asteraceae,Disk difüzyon,Mikro dilüsyon

Antibacterial Activity Determination of Different Species Belonging to Asteraceae Family

Abstract

The use of herbs as alternative medicine has increased dramatically in the last years. In recent years, due to the harmful effects of chemical drugs are frequently observed antioxidant, antibacterial and antifungal properties of extracts which derived from plants and they have been used in medicine. In this study, aim was investigated the antibacterial activities of some weed species which distributed to ecologically for different conditions. The antibacterial activities of Anthemis tinctorial L., Matricaria chamomilla L. and Achillea biebersteinii Afan. which belonging to Asteraceae family from Amasya province were determined by using disc diffusion and micro dilution methods. The methanolic extracts of these weeds were prepared with Soxhlet Extractor. The extracts were tested against standard strains of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella enteritidis. In this study, it was observed that weed extracts had antibacterial effects when they compared with control antibiotic group. the bacteria to be tested are sensitive to the control group antibiotics. According to the results of disc diffusion method, the highest antibacterial effect was identified A. tinctorial, M. chamomilla and A. biebersteinii respectively. The microdilution method was studied concentration range from 6.25 - to 50 mg/mL. In this context, it can be considered that these weed species are used as an alternative treatment and can lead to later antibacterial studies.

Keywords

• Asteraceae,Disc diffusion,Micro dilution

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