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Parazit bitkilerden (Orobanche ramosa L. Cuscuta campestris Yunck. ve Viscum album L.) elde edilen ekstratların bazı bitki patojeni funguslara karşı antifungal etkileri

Year 2017, Volume: 20 Issue: 1, 61 - 69, 01.02.2017

Abstract

Günümüzde kullanılan pestisitlerin olumsuz etkileri nedeniyle yeni alternatif kontrol yöntemleri bulmak önem kazanmıştır. Bu çalışmada, Alternaria solani, Monilinia fructigena, Fusarium oxysporum f. sp. radicis-lycopersici (FORL)'ye karşı üç parazit bitki türü olan Orabanche ramosa, Viscum album ve Cuscuta campestris'in antifungal aktiviteleri araştırılmıştır. Antifungal aktivite çalışmalarında parazit bitkilerden elde edilmiş methanol ekstraktları kullanılmıştır. Bu çalışmalar agar petri yöntemi kullanılarak belirlenmiştir. Bitki patojenlerine karşı ekstraktların 2, 5, 10 ve 20 mg ml-1 dozları kullanılmıştır. Denemeler 4 tekerrürlü olarak ve 2 kez tekrarlanmıştır. Elde edilen sonuçların yüzde miselyum gelişim engelleme (MGE) oranları ve letal dozları belirlenmiştir. Bu sonuçlara göre, her bitki ekstraktı belirgin bir şekilde antifungal aktivite göstermiştir. Doz miktarı arttıkça aktivite oranları da artış göstermiştir. Antifungal aktivite çalışmalarının sonuçlarına göre, ekstraktların 20 mg ml-1 dozlarındaki en yüksek etkiler, C. campestris’de A. solani'ye karşı %90, V. album’da M. fructigena'ya karşı %78, C. campestris'de FORL’ye karşı %76 oranlarında bulunmuştur. LD90 değerleri bitki patojenlerine karşı, O. ramosa'da 230.6-58.4, V. album’da 128.6-51.3 ve C. campestris'de ise 43.5-38.9 mg ml-1 arasında hesaplanmıştır. Ayrıca, parazit bitkilerden elde edilen metanol ekstratlarının ilk kez bu çalışmayla bitki patojenlerinin kontrolünde antifungal aktivite çalışmaları araştırılmıştır. Sonuç olarak, ortamdaki antifungal maddelerin etkinliklerinin tanımlanması sonucunda, daha ucuz ve çevre dostu alternatif maddelerin geliştirilmesi kolaylaşacaktır

References

  • Alice D and Rao AV (1987). Antifungal effects of plant etracts on Drechslera oryzae in rice. International Rice Research Newsletter 12(2):28. RPP. 67(2):758 Anonim (2016a). Dünya nüfusu https://tr.wikipedia.org/wiki/D%C3%BCnya_n%C3%BCfusu (Erişim tarihi 04.05.2017).
  • Anonim (2016b). Current World population. www.worldmeters.info/world-population/ (Erişim tarihi 01.05.2017)
  • Benhamou N, Lafontaine PJ, Nicole M (1994). Induction of systemic resistance to Fusarium crown and root rot in tomato plants by seed treatment with chitosan. Phytopathology 84:1432–1444.
  • Buza NL, Krinitsyna AA, Protsenko MA and Vartapetyan VV (2004). Intensity of apple fruit attack by brown rot fungus Monilia fructigena during ripening. Mikologiya i Fitopatologiya, 38(5):62-67.
  • Cramer HH (1967). Pflanzenschutz und Welternte. Pflanzenschutz Nachrichten Bayer Leverkusen, 20: 1-523.Ertürk Ö, Katı H, Yaylı N, Demirbağ Z (2003). Antimicrobial Activity of Viscum albumL. Subsp. abietis (Wiesb). Turk J Biol 27 (2003) 255-258.
  • Gatto MA, Ippolito A, Linsalata V, Cascarano NA, Nigro F, Vanadia S, Venere DD (2011).
  • Activity of extracts from wild edible hers against postharvest fungal diseases of fruit and vegetables. Postharvest Biology and Technology Vol.61, Issue1, P 72-82
  • Hussain MA, Khan MQ, Hussain N and Habib T (2011). Antibacterial and antifungal potential of leaves and twigs of Viscum album L. Journal of Medicinal Plants Research Vol. 5(23), pp. 5545-5549.
  • Jagtap MD, Asabe AS, Telave AB, Mali BS, Chavan SJ and Kanade MB (2014). Antifungal potential of Cuscuta reflexa Roxb. Central European Journal of Experimental Biology, 2014, 3(3):30-32
  • Kadıoğlu İ, Yanar Y (2004). Allelopathic Effects of Plant Extracts Against Seed Germination of Some Weeds, Asian J. of Plant Sciences, 3 ;4: 472-475, 2004.
  • Malik MS, Sanfwan NK, Dhindsa KS and Bhatti DS (1988). Nematicidal activity of extracts of Xanthium strumarium. Weed Abstr. 37(5):1673
  • Manirujjaman M, Suchana S, Collet T, Nawshin LN & Chowdhury MAR (2016) Antimicrobial effects of ethanolic extracts from Cuscuta reflexa Roxb. (Convolvulaceae). International Journal of Pharmacognosy and Phytochemical Research, 8(6), pp. 930-932.
  • Mukhtar I, Atiq M, Hanan A, (2012). Antifungal activity of Cuscuta reflexa Roxb. Pakistan journal of phytopathology. Vol 24, Issue:2, p: 163-166.
  • Nascimento GGF, Locatelli J, Freitas PC, Silva GL (2000). Antıbacterıal Actıvıty Of Plant Extracts And Phytochemıcals On Antıbıotıc-Resıstant Bacterıa. Brazilian Journal of Microbiology (2000) 31:247-256
  • Nemli Y, Kaya İ, Tamer ŞR (2015). Cuscuta campestris Yunck. S: 271-282. Türkiye istilaci bitkiler kataloğu, editör Hüseyin Önen, t.c. gida, tar. Ve hay. Bakanliği. Tagem, Bit. Sağ. Araş. Daire Başk., Ankara, ISBN: 978-605-9175-05-0
  • Nwosu MO, Okafor Jl (1995). Preliminary studies of the antifungal activites of some medicinal plants against Basidiobolus and some other pathogenic fungi. Mycoses 38, 191-195.
  • Onaran A and Yılar M (2012). Antifungal activity of Trachystemon orientalis L. aqueous extracts against plant pathogens. J. Food Agrıc. Environ. 10 (3&4), pp. 287-291.
  • Orhan DD, Özçelik D, Özgen S, Ergun F (2010). Antibacterial, antifungal and antiviral activities of some flavonoids. Microbiological Research Vol 165, Issue 6, p 496-504.
  • Özer Z, Kadıoğlu İ, Önen H, Tursun N (2003). Herboloji (Yabancı Ot Bilimi) Cilt 1. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Yayınları No:20 Kitaplar Serisi No:10, 263 sayfa
  • Özer Z, Önen H, Tursun N, Uygur FN (1999). Türkiye’nin Bazı Önemli Yabancı Otları (Tanımları ve Kimyasal Savaşımları). Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Yayınları N:38, Kitap Serisi No:16, 408 sayfa.
  • Pandey DK, Tripathi NN, Tripathi RD, Dixit SN, (1982). Fungitoxic and phytotoxic properties of essential oil of Hyptis suaveolens. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz, 89:344–349, 1982.
  • Rice EL (1984). Allelopathy. Second Editions. Acedemy Pres Inc. Ltd., London.
  • Sengul M, Yıldız H, Gungor N, Cetin B, Eser Z and Ercisli S (2009). Total phenolic content, antioxidat and antimicrobial activities of some medicinal plants. Pak. J Pharm Sci. 2009
  • Jan;22(1):102-6. Yazıcı S, Yanar Y and Karaman I (2011). Evaluation of bacteria for biological control of early blight disease of tomato. Afr. J. Biotechnol. 10;1573–1577.

Antifungal activity of parasitic plant (Orobanche ramosa L. Cuscuta campestris Yunck. and Viscum album L.) extracts against some plant pathogenic fungi

Year 2017, Volume: 20 Issue: 1, 61 - 69, 01.02.2017

Abstract

Because of the adverse effects of pesticides used today, it has become important to find new alternative control methods. In this study, antifungal activities of three parasitic plants species, Orabanche ramosa, Viscum album and Cuscuta campestris, were investigated against Alternaria solani, Monilinia fructigena, Fusarium oxysporum f. sp. radicis-lycopersici (FORL) plant pathogens. Methanol extracts derived from parasitic plants were used in antifungal activity studies. These studies were conducted with the agar plate method. Extract doses of 2, 5, 10 and 20 mg ml-1 used against plant pathogens. Experiments were set up four replicates and repeats 2 times. Percentage of mycelial growth inhibition (MGI) and lethal doses were determined according to results. According to these results, each plant extract showed noticeable antifungal activity. As the amount of the dose increased, the activity rates increased. As a result of antifungal activity studies, the highest effects were observed at 20 mg ml-1doses of plant extracts against A. solani, with rate of 90% in C. campestris, 78% in V. album against M. fructigena, and 76% against FORL in C. campestris. LD90 values were calculated as 230.6 to 58.4 in O. ramosa, 128.6 to 51.3 in V. album, and 43.5 to 38.9 mg ml-1 in C. campestris against plant pathogens. In addition, with the data collected from this study, the utilization of methanol extracts obtained from parasitic plants in control of plant pathogens were investigated for the first time. As a result, the identification of the activities of antifungal substances in the environment will facilitate to the discovery of cheaper and environmentally friendly alternative substances

References

  • Alice D and Rao AV (1987). Antifungal effects of plant etracts on Drechslera oryzae in rice. International Rice Research Newsletter 12(2):28. RPP. 67(2):758 Anonim (2016a). Dünya nüfusu https://tr.wikipedia.org/wiki/D%C3%BCnya_n%C3%BCfusu (Erişim tarihi 04.05.2017).
  • Anonim (2016b). Current World population. www.worldmeters.info/world-population/ (Erişim tarihi 01.05.2017)
  • Benhamou N, Lafontaine PJ, Nicole M (1994). Induction of systemic resistance to Fusarium crown and root rot in tomato plants by seed treatment with chitosan. Phytopathology 84:1432–1444.
  • Buza NL, Krinitsyna AA, Protsenko MA and Vartapetyan VV (2004). Intensity of apple fruit attack by brown rot fungus Monilia fructigena during ripening. Mikologiya i Fitopatologiya, 38(5):62-67.
  • Cramer HH (1967). Pflanzenschutz und Welternte. Pflanzenschutz Nachrichten Bayer Leverkusen, 20: 1-523.Ertürk Ö, Katı H, Yaylı N, Demirbağ Z (2003). Antimicrobial Activity of Viscum albumL. Subsp. abietis (Wiesb). Turk J Biol 27 (2003) 255-258.
  • Gatto MA, Ippolito A, Linsalata V, Cascarano NA, Nigro F, Vanadia S, Venere DD (2011).
  • Activity of extracts from wild edible hers against postharvest fungal diseases of fruit and vegetables. Postharvest Biology and Technology Vol.61, Issue1, P 72-82
  • Hussain MA, Khan MQ, Hussain N and Habib T (2011). Antibacterial and antifungal potential of leaves and twigs of Viscum album L. Journal of Medicinal Plants Research Vol. 5(23), pp. 5545-5549.
  • Jagtap MD, Asabe AS, Telave AB, Mali BS, Chavan SJ and Kanade MB (2014). Antifungal potential of Cuscuta reflexa Roxb. Central European Journal of Experimental Biology, 2014, 3(3):30-32
  • Kadıoğlu İ, Yanar Y (2004). Allelopathic Effects of Plant Extracts Against Seed Germination of Some Weeds, Asian J. of Plant Sciences, 3 ;4: 472-475, 2004.
  • Malik MS, Sanfwan NK, Dhindsa KS and Bhatti DS (1988). Nematicidal activity of extracts of Xanthium strumarium. Weed Abstr. 37(5):1673
  • Manirujjaman M, Suchana S, Collet T, Nawshin LN & Chowdhury MAR (2016) Antimicrobial effects of ethanolic extracts from Cuscuta reflexa Roxb. (Convolvulaceae). International Journal of Pharmacognosy and Phytochemical Research, 8(6), pp. 930-932.
  • Mukhtar I, Atiq M, Hanan A, (2012). Antifungal activity of Cuscuta reflexa Roxb. Pakistan journal of phytopathology. Vol 24, Issue:2, p: 163-166.
  • Nascimento GGF, Locatelli J, Freitas PC, Silva GL (2000). Antıbacterıal Actıvıty Of Plant Extracts And Phytochemıcals On Antıbıotıc-Resıstant Bacterıa. Brazilian Journal of Microbiology (2000) 31:247-256
  • Nemli Y, Kaya İ, Tamer ŞR (2015). Cuscuta campestris Yunck. S: 271-282. Türkiye istilaci bitkiler kataloğu, editör Hüseyin Önen, t.c. gida, tar. Ve hay. Bakanliği. Tagem, Bit. Sağ. Araş. Daire Başk., Ankara, ISBN: 978-605-9175-05-0
  • Nwosu MO, Okafor Jl (1995). Preliminary studies of the antifungal activites of some medicinal plants against Basidiobolus and some other pathogenic fungi. Mycoses 38, 191-195.
  • Onaran A and Yılar M (2012). Antifungal activity of Trachystemon orientalis L. aqueous extracts against plant pathogens. J. Food Agrıc. Environ. 10 (3&4), pp. 287-291.
  • Orhan DD, Özçelik D, Özgen S, Ergun F (2010). Antibacterial, antifungal and antiviral activities of some flavonoids. Microbiological Research Vol 165, Issue 6, p 496-504.
  • Özer Z, Kadıoğlu İ, Önen H, Tursun N (2003). Herboloji (Yabancı Ot Bilimi) Cilt 1. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Yayınları No:20 Kitaplar Serisi No:10, 263 sayfa
  • Özer Z, Önen H, Tursun N, Uygur FN (1999). Türkiye’nin Bazı Önemli Yabancı Otları (Tanımları ve Kimyasal Savaşımları). Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Yayınları N:38, Kitap Serisi No:16, 408 sayfa.
  • Pandey DK, Tripathi NN, Tripathi RD, Dixit SN, (1982). Fungitoxic and phytotoxic properties of essential oil of Hyptis suaveolens. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz, 89:344–349, 1982.
  • Rice EL (1984). Allelopathy. Second Editions. Acedemy Pres Inc. Ltd., London.
  • Sengul M, Yıldız H, Gungor N, Cetin B, Eser Z and Ercisli S (2009). Total phenolic content, antioxidat and antimicrobial activities of some medicinal plants. Pak. J Pharm Sci. 2009
  • Jan;22(1):102-6. Yazıcı S, Yanar Y and Karaman I (2011). Evaluation of bacteria for biological control of early blight disease of tomato. Afr. J. Biotechnol. 10;1573–1577.
There are 24 citations in total.

Details

Other ID JA85AE64EJ
Journal Section Research Article
Authors

Bahadır Şin This is me

İzzet Kadıoğlu This is me

Abdurrahman Onaran This is me

Publication Date February 1, 2017
Published in Issue Year 2017 Volume: 20 Issue: 1

Cite

APA Şin, B., Kadıoğlu, İ., & Onaran, A. (2017). Antifungal activity of parasitic plant (Orobanche ramosa L. Cuscuta campestris Yunck. and Viscum album L.) extracts against some plant pathogenic fungi. Turkish Journal of Weed Science, 20(1), 61-69.

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