Effects of Ischemic Precondition and K-ATP Channel Opener Pinacidil on Intestinal Ischemia-Reperfusion Injury

Year 2009, Volume: 3 Issue: 1, 7 - 14, 19.03.2009

Saadet Akturan

Abstract

To investigate and to compare the effect of ischemic precondition and K-ATP channel activating agent pinacidil, showing anti-ischemic effects which are similar to ischemic pre-conditioning, on ischemic injury in experimental intestinal ischemia reperfusion model. Five groups were made up each including 6 Wistar Albino rat (200- 250 gr). Only laparotomy performed on the control group (group I). In I/R (group II), ischemia was induced with vascular clempage for 40 minutes to proximal superior mesentric artery, and then reperfusion was induced for 90 minutes with declampage. Five minutes before I/R period, 0,1 mg/kg pinacidil was given intravenously to Pin.+I/R (group III), in addition to group II. In IÖ+IR group (group IV), before I/R period, 10 minutes ischemia and then 10 minutes reperfusion was performed, in addition to group II. At the fifth minute of the reperfusion stage. Pin. were given to IÖ+Pin.+1/R group (group V), in addition to group IV. The level of malondialdehit (MDA) was measured in blood and terminal ileum tissue by performing relaparatomy. Furthermore, microbiological study was carried out in blood and mesentric lymph node. Histopathologically terminal ileum in the groups was investigated. Bacterial translocation (BT) to MLN and the level of tissue MDA in the I/R group were significantly higher than the other grups. In the IÖ+IR group, BT to MLN, levels of tissue and plasma MDA were significantly lower than I/R group. In the Pin.+I/R group, BT to MLN, level of tissue MDA were significantly lower than I/R group. In the IÖ+Pin.+1/R group, BT to MLN, level of tissue MDA, and histopathologically ileal ischemia score were significantly lower than I/R group. There was no significant difference in the parameters among IÖ+I/R, Pin.+I/R and IÖ+Pin. I/R groups. Pin. and IÖ decreased lipid peroxidation, deterioration in ileum morphology, and BT, resulting from intestinal I/R. Given Pin. and IO together has no superiority on giving them each alone.

Keywords

schemia-reperfusion, pinacidil, ischemic precondition

İskemik Önkoşullanmanın ve K-ATP Kanal Açıcı Ajan Pinacidil’in Intestinal İskemi-Reperfüzyon Hasarına Etkisi

Abstract

Deneysel intestinal iskemi-reperfüzyon (I/R) modelinde, iskemik önkoşullanmanın (IÖ) ve K-ATP kanallarını spesifik olarak açarak önkoşullanmaya benzer antiiskemik etki gösteren pinacidil’in (Pin.) iskemik hasar üzerine etkilerini incelemek ve karşılaştırmaktır. Her biri 6 Wistar Albino rat (200- 250 gram) içeren 5 grup oluşturuldu. Kontrol (I. grup) grubuna sadece laparotomi yapıldı. I/R (II. grup) grubuna süperior mezenterik arterin aort çıkışına 40 dakika vasküler klempaj ile iskemi yapılıp, ardından klemp açılarak 90 dakika reperfüzyon sağlandı. Pin.+I/R (III. grup) grubuna II. gruba ek olarak I/R periyodundan 5 dakika önce 0.1 mg/kg pinacidil in-travenöz (İV) puşe olarak yapıldı. IÖ+IR (IV. grup) grubuna II. gruba ek olarak I/R periyodundan önce 10 dakika iskemi, 10 dakika reperfüzyon yapıldı. IÖ+Pin.+I/R (V. grup) grubuna IV. gruba ek olarak lÖ’nın reperfüzyon safhasının 5. dakikasında pinacidil uygulandı. Yeniden laparatomi yapılarak kanda ve terminal ileum dokusunda malondialdehit (MDA) düzeyine bakıldı. Kanda ve mezenterik lenf nodu (MLN)’da mikrobiyolojik çalışma yapıldı. Terminal ileum dokusu histopatolojik olarak incelendi. I/R grubunda MLN’e bakteriyel translokasyon (BT)’un ve doku MDA düzeyinin diğer gruplardan yüksek olması istatistiksel olarak anlamlı bulundu. IÖ+IR grubunda MLN’e BT, doku ve serum MDA düzeyleri I/R grubundan anlamlı olarak daha düşüktü. Pin.+I/R grubunda MLN’e BT ve doku MDA düzeyi I/R grubundan anlamlı olarak daha düşük bulundu. IÖ+Pin.+I/R grubunda MLN’e BT’nin, doku MDA düzeyinin ve terminal ileum histopatolojik skorlamasının I/R grubundan anlamlı olarak daha düşük olduğu görüldü. IÖ+I/R, Pin.+I/R ve lÖ+Pin. I/R grupları arasında yukarıdaki parametrelerde anlamlı farklılık saptanmadı. Pin. ve IÖ, intestinal I/R sonucu gelişen lipit peroksidasyonunu, ileum morfolojisindeki bozulmayı ve BT’i azaltmaktadır. Pin. ve îö’nın birlikte kullanılmasının, yalnız Pin. ya da yalnız IÖ ile sağlanan korumaya belirgin bir üstünlüğü yoktur.

Keywords

İskemi-reperfüzyon, pinacidil, iskemik önkoşullanma

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