Anti-inflammatory activity of acetonic extract of Pistacia lentiscus fruits

Abstract

acute inflammation, Croton oil-induced ear edema and ear pouch in mice. Croton oil-induced ear edema mice was conducted in three ways: 1. Simultaneously use of the treatment with the irritant agent: croton oil-induced ear edema was performed according to the method of Manga et al. (2004). Cutaneous inflammation was induced to the inner surface of the right ear of mice (7 mice each group) by application of 15 ȝL of acetone containing 80 ȝg of Croton oil as irritant. Treated animals received topically 3 mg/ear of acetonic extract of P. lentiscus fuits. Indomethacin as reference drug was applied topically (0.5 mg/ear). The control group received topically Croton oil alone. The thickness of ears was measured before and 6 h after induction of inflammation using a digital micrometer. The micrometer was applied near the tip of the ear just distal to the cartilaginous ridges, and the thickness was recorded in micrometers. To minimize technique variations, a single investigator performed the measurements throughout each experiment. The edema was expressed as an increase in the ear thickness due to Croton oil application. The inhibition of the inflammation was calculated using the following equation: Inhibition = (ǻT – ǻE / ǻ T) x 100, were ǻT: edema size of the control and ǻE: edema size of the treated group by the extract. 2. Topical pretreatment 1 hour before the induction of inflammation: A volume of 15 ȝl of acetone-water solution (1:1) containing 3 mg of extract or 0.5 mg of indomethacin were applied topically on the inner surface of the right ear of mice. One hour after application of the treatment, 15 ȝl l of acetone-water solution (1:1) containing 80 mg of Croton oil was applied locally on the inner surface of the right ear of each mouse. The control mice received only 15 ȝl of the solution of Croton oil. The ear thickness was measured before the treatment and then 4h and 6h after the induction of inflammation. 3. Oral pretreatment 1 hour before the induction of inflammation: Three groups of mice were respectively received orally 0.2 ml of saline solution (control group), 300 mg/kg of acetonic extract of P. lentiscus fruits and 50

Keywords

• Inflammation, anti-inflammatory, Pistacia lentiscus, polyphenols, flavonoids

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