The Microscopical Evaluation of Age Dependent Modifications in Rat Ileum

Abstract

Aim: In this study, we aimed to determine changes inhe number of villi, height of villi, width of villi and the number of goblet cells in rat ileum. Materials and Methods: For this purpose, 24 female Sprague Dawley rats were divided into 3 groups each containing 8 rats. Tissue samples were removed on the 21st day, 60th day and at 19 months. Sections were stained by the hematoxylin-eosin and periodic acid Schiff (PAS). The number of villi, height of villi, width of villi and number of goblet cell were determined by Leica Q Win image analysis system. Results: In the present study, the mean number of villi was 9.50±0.202 at 21 days old, 7.50±0.164 at 60 days old and 7.07±0.180 at 19 months old rats. There was a statistically significant difference between 21 days old and 60 days old groups, and 21 days old and 19 months old groups (p=0.0001). We have detected that the mean villi heights as 320.73±3.801 μm at 21 days old, 509.34±4.207 μm at 60 days old and 381.09±3.528 μm at 19 months old rats. All three groups had statistically significant differences compared with each other (p=0.0001). We have detected that the mean villi width as 86.75±1.665 μm at 21 days old, 110.08±1.331 μm at 60 days old and 100.51±2.140 μm at 19 months old rats. All three groups had a statistically significant difference compared with each other (p=0.0001). We have detected that mean number of goblet cells as 14.83±0.184 at 21 days old, 24.42±0.239 at 60 days old and 18.70±0.241 at 19 months old rats. All three groups had a statistically significant difference compared with each other (p=0.0001). Conclusion: The collected data shall provide a reference point for future studies. Key words: Ileum; Goblet Cell; Villi; Rat; Histomorphology.

Keywords

• Ileum; Goblet Cell; Villi; Rat; Histomorphology.

Sıçan İleumunda Meydana Gelen Yaşa Bağlı Değişimlerin Mikroskopik Olarak İncelenmesi

Abstract

Amaç: Bu çalışmada, sıçan ileumunda yaşa bağlı olarak değiştiği düşünülen villusların sayısını, yüksekliğini, genişliğini ve goblet hücre sayısını incelemeyi amaçladık. Gereç ve Yöntem: Bu amaçla 24 adet dişi Sprague Dawley cinsi sıçanlar her grupta 8'er sıçandan oluşan 3 gruba ayrıldılar. Sıçanlar 21 günlük, 60 günlük ve 19 aylık olduklarında ileum dokuları çıkarıldı. Kesitler hematoksilen-eosin (H-E) ve periodic acid Schiff (PAS) ile boyandı. Leica Q Win görüntü analiz sistemi kullanılarak ortalama villus sayısı, villus yüksekliği, villus genişliği ve villuslardaki ortalama goblet hücre sayısı belirlendi. Bulgular: Bu çalışmada, ortalama villus sayısını 21 günlük grupta 9.50±0.20, 60 günlük grupta 7.50±0.16 ve 19 aylık grupta 7.07±0.18 olarak tespit ettik. 21 günlük grup ile 60 günlük grup ve 21 günlük grup ile 19 aylık grup arasında istatistiksel olarak anlamlı fark vardı (p=0.0001). Villus yüksekliğini 21 günlük grupta 320.73±3.80 μm, 60 günlük grupta 509.34±4.20 μm ve 19 aylık grupta 381.09±3.52 μm olarak tespit ettik. Grupları birbirleriyle karşılaştırdığımızda villus yüksekliği açısından aralarında istatistiksel olarak anlamlı fark vardı (p=0.0001). Villus genişliğini 21 günlük sıçanlarda 86.75±1.66 μm, 60 günlük sıçanlarda 110.08±1.33 μm ve 19 aylık sıçanlarda 100.51±2.14 μm olarak tespit ettik. Grupları birbirleriyle karşılaştırdığımızda villus genişliği açısından aralarında istatistiksel olarak anlamlı fark vardı (p=0.0001). Her villustaki ortalama goblet hücre sayısını 21 günlük grupta 14.83±0.18, 60 günlük grupta 24.42±0.23 ve 19 aylık grupta 18.70±0.24 olarak tespit ettik. Grupları birbirleriyle karşılaştırdığımızda aralarında istatistiksel olarak anlamlı fark vardı (p=0.0001). Sonuç: Elde edilen veriler sindirim sistemi ile ilgili çalışmalara referans oluşturacaktır. Anahtar kelimeler: İleum; Goblet Hücresi; Villus; Sıçan; Histomorfoloji.

Keywords

• İleum; Goblet Hücresi; Villus; Sıçan; Histomorfoloji.

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