The Microscopical Evaluation of Age Dependent Modifications in Rat Ileum

Year 2012, Volume: 19 Issue: 4, 237 - 245, 01.08.2012

Hülya Elbe Meltem Kuruş Alper Kazancı Ali Otlu

Abstract

Aim: In this study, we aimed to determine changes inhe number of villi, height of villi, width of villi and the number of goblet cells in rat ileum. Materials and Methods: For this purpose, 24 female Sprague Dawley rats were divided into 3 groups each containing 8 rats. Tissue samples were removed on the 21st day, 60th day and at 19 months. Sections were stained by the hematoxylin-eosin and periodic acid Schiff (PAS). The number of villi, height of villi, width of villi and number of goblet cell were determined by Leica Q Win image analysis system. Results: In the present study, the mean number of villi was 9.50±0.202 at 21 days old, 7.50±0.164 at 60 days old and 7.07±0.180 at 19 months old rats. There was a statistically significant difference between 21 days old and 60 days old groups, and 21 days old and 19 months old groups (p=0.0001). We have detected that the mean villi heights as 320.73±3.801 μm at 21 days old, 509.34±4.207 μm at 60 days old and 381.09±3.528 μm at 19 months old rats. All three groups had statistically significant differences compared with each other (p=0.0001). We have detected that the mean villi width as 86.75±1.665 μm at 21 days old, 110.08±1.331 μm at 60 days old and 100.51±2.140 μm at 19 months old rats. All three groups had a statistically significant difference compared with each other (p=0.0001). We have detected that mean number of goblet cells as 14.83±0.184 at 21 days old, 24.42±0.239 at 60 days old and 18.70±0.241 at 19 months old rats. All three groups had a statistically significant difference compared with each other (p=0.0001). Conclusion: The collected data shall provide a reference point for future studies. Key words: Ileum; Goblet Cell; Villi; Rat; Histomorphology.

Keywords

Ileum; Goblet Cell; Villi; Rat; Histomorphology.

Sıçan İleumunda Meydana Gelen Yaşa Bağlı Değişimlerin Mikroskopik Olarak İncelenmesi

Abstract

Amaç: Bu çalışmada, sıçan ileumunda yaşa bağlı olarak değiştiği düşünülen villusların sayısını, yüksekliğini, genişliğini ve goblet hücre sayısını incelemeyi amaçladık. Gereç ve Yöntem: Bu amaçla 24 adet dişi Sprague Dawley cinsi sıçanlar her grupta 8'er sıçandan oluşan 3 gruba ayrıldılar. Sıçanlar 21 günlük, 60 günlük ve 19 aylık olduklarında ileum dokuları çıkarıldı. Kesitler hematoksilen-eosin (H-E) ve periodic acid Schiff (PAS) ile boyandı. Leica Q Win görüntü analiz sistemi kullanılarak ortalama villus sayısı, villus yüksekliği, villus genişliği ve villuslardaki ortalama goblet hücre sayısı belirlendi. Bulgular: Bu çalışmada, ortalama villus sayısını 21 günlük grupta 9.50±0.20, 60 günlük grupta 7.50±0.16 ve 19 aylık grupta 7.07±0.18 olarak tespit ettik. 21 günlük grup ile 60 günlük grup ve 21 günlük grup ile 19 aylık grup arasında istatistiksel olarak anlamlı fark vardı (p=0.0001). Villus yüksekliğini 21 günlük grupta 320.73±3.80 μm, 60 günlük grupta 509.34±4.20 μm ve 19 aylık grupta 381.09±3.52 μm olarak tespit ettik. Grupları birbirleriyle karşılaştırdığımızda villus yüksekliği açısından aralarında istatistiksel olarak anlamlı fark vardı (p=0.0001). Villus genişliğini 21 günlük sıçanlarda 86.75±1.66 μm, 60 günlük sıçanlarda 110.08±1.33 μm ve 19 aylık sıçanlarda 100.51±2.14 μm olarak tespit ettik. Grupları birbirleriyle karşılaştırdığımızda villus genişliği açısından aralarında istatistiksel olarak anlamlı fark vardı (p=0.0001). Her villustaki ortalama goblet hücre sayısını 21 günlük grupta 14.83±0.18, 60 günlük grupta 24.42±0.23 ve 19 aylık grupta 18.70±0.24 olarak tespit ettik. Grupları birbirleriyle karşılaştırdığımızda aralarında istatistiksel olarak anlamlı fark vardı (p=0.0001). Sonuç: Elde edilen veriler sindirim sistemi ile ilgili çalışmalara referans oluşturacaktır. Anahtar kelimeler: İleum; Goblet Hücresi; Villus; Sıçan; Histomorfoloji.

Keywords

İleum; Goblet Hücresi; Villus; Sıçan; Histomorfoloji.

References

• Wang JX, Peng KM. Developmental morphology of the small intestine of African ostrich chicks. Poultry Science 2008;87:2629-35.
• Ergün E, Ergün L, Özen A, Kürüm A. Ankara tavşanı ince bağırsağında paneth hücrelerinin morfolojisi, histokimyası ve ince yapısı üzerinde çalışmalar. Ankara Üniv Vet Fak Derg 2009;56:25-30.
• P. de Santa Barbara, van den Brink GR, Roberts DJ. Development and differentiation of the intestinal epithelium. Cell Mol Life Sci 2003;60:1322-32.
• Rubin DC. Intestinal morphogenesis. Curr Opin Gastroenterol 2007;23:111-4.
• Erdoğan D, Hatiboğlu MT, Görgün M, ve ark. Özel Histoloji;Ankara: Hatiboğlu Yayınları. 1996.
• Gartner LP, Hiatt LJ. Color Textbook of Histology; Second edition. Philadelphia: WB Saunder Co. 2001.
• Junqueria LC, Carneiro J. Temel Histoloji (Türkçe Çeviri); İstanbul: Nobel Tıp Kitabevleri. 2009.
• Ross MH, Pawlina W. Histology A Text and Atlas; 5th Edition. US: Lippincott Williams&Wilkins. 2006.
• Hernandes L, Marques da Silva Pereira LC, Alvares AP. Goblet cell number in the ileum of rats denervated during suckling and weaning. Biocell 2003;27:347-51.
• Vigueras RM, Reyes G, Rojas-Castañeda J, Rojas P, Hernández R. Histological characteristics of the intestinal mucosa of the rat during the first year of life. Lab Anim 1999;33:393-400.
• Drozdowski L, Thomson ABR. Aging and the intestine. World J Gastroenterol 2006;12:7578-84.
• Bozkurt M, Sandıkçı M. Farklı yaşlardaki civcivlerin barsak villus boyu ve çapı ile kadeh hücresi ve Mitotik hücre sayılarındaki değişimler. YYU Veteriner Fakültesi Dergisi 2009;20:5-9.
• Brown DC, Maxwell CV, Erf GF, Davis ME, Singh S, Johnson ZB. The influence of different management systems and age on intestinal morphology, immune cell numbers and mucin production from goblet cells in post- weaning pigs. Vet Immunol and Immunopathol 2006;111:187-98.
• Clarke RM. Does the number of intestinal villi grow with the rat ? J Anat 1971;109:352.
• Clarke RM. The effect of growth and of fasting on the number of villi and crypts in the small intestine of the albino rat. J Anat 1972;112:27-33.
• Clarke RM. The effects of age on mucosal morphology and epithelial cell production in rat small intestine. J Anat 1977;123:805-11.
• Kapadia S, Baker SJ. The Effects of alterations in villus shape on intestinal mucosal surface area and the crypts. Digestion 1972;14: 256-68.
• Borghi F, Petrino R, Bellora P, Gattuso G, Di Napoli A, Cellino G, et. al. Postnatal development of intestinal villi in the rat. determination of villus size gradient. Panminerva Med 1994;36:149.
• Fry RJM, Lesher S, Kohn HI. Influence of age on the transit time of cells of mouse intestinal epithelium. Lab Invest 1962;11:289-93.
• Warren PM, Pepperman MA, Montgomery RD. Age changes in small intestinal mucosa. Lancet 1978;2:849-50. 21. Warren R. Serosal and mucosal dimensions at different levels of the dog’s small intestine. Anat Rec 1939;75:427- 37.
• Iji PA, Saki A, Tivey DR. Body and intestinal growth of broiler chicks on a commercial starter diet. intestinal weight and mucosal development. British Poult Sci 2001;42:505-13.
• Smits CH, Maarssen CA, Mouwen VM, Koninkx JG, Beynen AC. The antinutritive effect of a carboxymethylcellulose with high viscosity on lipid digestibility in broiler chickens is not associated with mucosal damage. J Anim Phiysiol and Anim Nutr 2000;83:239-45.
• Sorkun HÇ, Özdamar S. Sıçan ince ve kalın bağırsaklarının gelişimi ve gastrin hücre dağılımı. Türkiye Klinikleri J Med Sci 2005;25:10-19.
• Martin K, Kirkwood TB, Potten CS. Age changes in stem cells of murine small intestinal crypts. Exp Cell Res 1998;241:316-23.
• Holt P, Pascal R, Kotler D. Effect of aging upon small intestinal structure in the fischer rat. J Gerontol 1984;39:642-7.
• Hollmann KH. The fine structure of goblet cells in the rat intestine. Ann N Y Acad of Sci 1963;106:545-54.
• Freeman JA. Goblet cell fine structure. Anat Rec 1966;154:121-47.
• Yunus M, Horii Y, Makimura S Smith AL. Murine goblet cell hypoplasia during eimeria pragensis infection is ameliorated by clindamycin treatment. J Vet Med Sci 2005;67:311-5.
• Paulus U, Loeffler M, Zeidler J, Owen G, Potten CS. The differentiation and lineage development of goblet cells in the murine small intestinal crypt: Experimental and modelling studies. J Cell Sci 1993;106:473-84.
• Dağ M, Kurus M, Söğütlü G. İskemi-reperfüzyon uygulanan sıçan jejunum goblet hücrelerinin morfolojik ve sayısal değişimi. Turkiye Klinikleri J Med Sci 2010;952:30:952-61.
• Forstner JF. Intestinal mucins in health and disease. Digestion 1978;17:234-63
• Specian RD, Oliver MG. Functional biology of intestinal goblet cells. Am J Physiol 1991;260:183-93.
• Sharma R, Schumacher U, Ronaasen V, Coates M. Rat intestinal mucosal responses to a microbial flora and different diets. Gut 1995;36:209-14.
• Rose MC. Mucins: structure, function and role in pulmonary diseases. Lung Cellular and Molecular Physiology 1992;263: 413-29.
• Luna LG. Methods for carbohydrates and mucoproteins. Manual of histologic staining methods of armed forces institute of pathology. McGraw-Hill Book Company: New York. 1968.
• Knospe C. Evidence for secretion channels in the gastric mucous sheet of the cat. Acta Anat 1991; 142: 1-5.
• Noyan A. Yaşamda ve Hekimlikte Fizyoloji. Meteksan Anonim Şirketi. Ankara. 2004.
• Liévin-Le Moalad V, Servin A. The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: Mucins, antimicrobial peptides, and microbiota. Clin Microbiol Rev 2006;19:315-37.
• Uni Z, Smirnov A, Sklan D. Pre and posthatch development of goblet cells in the broiler small intestine: Effect of delayed access to feed. Poultry Science 2003;82: 320-7.
• Deplancke B, Gaskins HR. Microbial modulation of innate defense: Goblet cells and the intestinal mucus layer. Am J Clin Nutr 2001;73:1131-41.
• McManus JFA. Histological and histochemical uses of periodic acid. Stain Technol. 1948;23:99.
• Prophet EB, Mills B, Arrington JB. Laboratory methods in histotechnology. American forces institute of pathology. 1. Edition, American Registry of Pathology, USA: 1992.
• Cummins AG, Steele TW, LaBrooy JT, Shearman DJ. Maturation of the rat small intestine at weaning: Change in epithelial cell kinetics, bacterial flora and mucosal immune activity. Gut 1988;29:1672-9.