The Histology of Digestive and Respiratory System Macrophages in Rats

Year 2008, Volume: 15 Issue: 4, 225 - 231, 01.08.2008

Aslı Çetin Feral Öztürk Mehmet Gül Mukaddes Eşrefoğlu Ali Otlu

Abstract

Purpose: The aim of this study was to examine the morphology of the connective tissue macrophages in digestive and respiratory systems, alveolar macrophages in lung and Kupffer cells in liver with light and electron microscopes, and to compare their histologic structures in order to observe their shared and different morphologic properties. Materials and Methods: In this study, eight male rats (200-280g) obtained from Inonu University Laboratory Animals Research Center were used. Six rats were injected with 3 ml trypan blue intraperitoneally everyday for seven days. Two of the rats were used as controls and no injections were performed. The tissues collected from the sacrified rats were fixed, processed and stained with routine techniques and examined with light and electron microscopes. Results: Macrophages were seen as large cells containing cytoplasmic blue granules, located in the submucosa and adventitia of tongue, sophagus, small and large intestine and in periton. In liver Kupffer cells were seen as small cells with blue granules located perisinusoidally. Macrophages were detected as small cells in trachea and lungs located around bronch, bronchiole and blood vessels. With electron microscope, they were observed as oval and rounded cells with irregular plasmalemma. Kupffer cells were star like cells showing short and long cytoplasmic extensions. Conclusion: We concluded that digestive and respiratory system macrophages were morphologically similar. Key words: Macrophage, Rat, Light Microscope, Electron Microscope.

Keywords

Macrophage, Rat, Light Microscope, Electron Microscope.

Sıçanların Sindirim ve Solunum Sistemlerindeki Makrofajların Histolojik Yapıları

Abstract

Amaç: Bu çalışma, sıçanların sindirim ve solunum sistemlerinde yerleşmiş bağ dokusu makrofajlarını, akciğerdeki alveoler makrofajları ve karaciğer Kupffer hücrelerini ışık ve elektron mikroskobik olarak incelemek ve histolojik yapılarını birbirleriyle karşılaştırarak, farklı ve ortak morfolojik özelliklerini belirlemek amacıyla yapılmıştır. Materyal ve Metod: Bu çalışmada İnönü Üniversitesi Deney Hayvanları Araştırma Laboratuvarından temin edilen sekiz adet Wistar cinsi erkek sıçan (200-280 gr) kullanıldı. Altı tanesine yedi gün süresince her gün intraperitoneal 3ml trypan blue solusyonu enjekte edildi. İki tanesi ise kontrol olarak kullanıldı, herhangi bir işlem uygulanmadı. Sakrifiye edilen sıçanlardan alınan dokular rutin tespit ve doku takibi sonrasında histokimyasal olarak boyanarak ışık ve elektron mikroskobunda incelendi. Bulgular: Makrofajlar periton, dil, özofagus, ince ve kalın barsak submukozası ve adventisyasında damarlar çevresine yerleşmiş mavi sitoplazmik granüller içeren büyük hücreler olarak gözlendi. Karaciğerde Kupffer hücreleri, perisinüzoidal yerleşmiş küçük mavi granüllü hücreler olarak izlendi. Makrofajlar, trakeada, akciğerlerde bronş, bronşiol ve kan damarları çevresinde yerleşmiş hücreler olarak gözlendi. Elektron mikroskopta makrofajlar, oval, yuvarlak, plazmalemması girinti ve çıkıntılı hücreler olarak görüldü. Kupffer hücreleri ise uzun ve kısa sitoplazmik uzantılara sahip yıldız şeklinde hücreler olarak izlendi. Sonuç: Sindirim ve solunum sistemlerine yerleşmiş olan makrofajların morfolojik olarak birbirlerine benzedikleri sonucuna vardık. Anahtar kelimeler: Makrofaj, Sıçan, Işık mikroskobu, Elektron mikroskobu.

Keywords

Makrofaj, Sıçan, Işık mikroskobu, Elektron mikroskobu.

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