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Lens Capsular Glutathione Level and Glutathione Peroxidase Activity Among Diabetic Patients

Year 2008, Volume: 15 Issue: 3, 139 - 143, 01.06.2008

Sinan Emre Burhan Ateş Ahmet Taşar Selim Doğanay

Abstract

Purpose: To evaluate the level of glutathione (GSH) and gluthathione peroxidase (GSH-Px) activity in the anterior lens capsule of patients with cataract in diabetic patients with meticulous control and in patients with senile cataract. Method: The anterior lens capsules in 18 eyes with diabetes under tight glycemic control with cataract (Group A) and in 26 eyes with senile cataract (Group B) were collected in phacoemulsification surgery. The specimens were thawed, centrifuged and the supernatants of the homogenate obtained from the samples were analyzed for the GSH level and GSH-Px enzyme activity. Results: Group A composed of 10 female and 8 male patients and mean age of patients was 60.3 ± 8.3 years. Group B composed of 11 female and 15 male patients and mean age of the patients was 63.9 ± 12.2 years. Mean of the measurements for GSH level and GSH-Px activity at anterior capsule were 82.4±16.5 vs. 97.5±12.5 nmol/mg and 27.0±9.7 vs. 36.9±9.3 mmol/mg protein, in group A and B, respectively. The differences between groups were without any statistical significance for both parameters(p>0.05). Conclusion: The detorious effect of diabetes on anti-oxidant mechanisms is a well known subject. Our results indicate that approximate level of GSH and GSH-Px activity between diabetics with tight glucose control group and age-matched control group may be the result of meticulous control of hyperglycemia. Key words: Cataract, Diabetes mellitus, Glutathione (GSH), Glutathione peroxidase (GSH-Px), Meticulous controlled diabetes

Keywords

Cataract Diabetes mellitus Glutathione (GSH) Glutathione peroxidase (GSH-Px) Meticulous controlled diabetes

References

  • Özmen B, Özmen B, Erkin E, et al. Lens superoxide dismutase and catalase activities in diabetic cataract. Clinical Biochemistry 2002; 35: 69-72.
  • Godin DV, Wohaieb SA, Garnett ME, Goumeniouk AD. Antioxidant enzyme alterations in experimental and clinical diabetes. Mol Cell Biochem. 1988 ; 84:223- 31.
  • Mileva M, Bakalova R, Zlateva G. Low-Intensity Laser Irradiation Does not Affect the Oxidative Stress in Experimental Cataract. Med. Laser Appl. 2004; 19:150-4.
  • Obara Y. The oxidative stress in the cataract formation. Nippon Ganka Gakkai Zasshi. 1995 ; 99:1303-41.
  • Ganea E, Harding JJ. Glutathione-related enzymes and the eye. Curr Eye Res. 2006;31:1-11.
  • Harding JJ. Free and protein-bound glutathione in normal and cataractous human lenses. Biochem J. 1970 ;117:957-60.
  • George S, Jyothi M, Mathew B, Shashidhar S. Changes in glutathione, glutathione- linked enzymes and hexose monophosphate shunt enzymes in senile cataract. Indian J Physiol Pharmacol. 2003 ;47:191-6.
  • Donma O, Yorulmaz E, Pekel H, Suyugül N. Blood and lens lipid peroxidation and antioxidant status in normal individuals, senile and diabetic cataractous patients. Curr Eye Res. 2002 ;25:9-16.
  • Doganay S, Borazan M, Iraz M, Cigremis Y. The effect of resveratrol in experimental cataract model formed by sodium selenite. Curr Eye Res. 2006 ;31:147-53.
  • Doganay S, Turkoz Y, Evereklioglu C, et al. Use of caffeic acid phenethyl ester to prevent sodium-selenite-induced cataract in rat eyes. J Cataract Refract Surg. 2002 ;28:1457-62.
  • Stitt AW. Advanced glycation: an important pathological event in diabetic and age related ocular disease. Br J Ophthalmol. 2001 ;85:746-53.
  • Blakytny R, Harding JJ. Glycation (non-enzymic glycosylation) inactivates glutathione reductase. Biochem J. 1992 ;288 :303-7.
  • Ortwerth BJ, Olesen PR. Glutathione inhibits the glycation and crosslinking of lens proteins by ascorbic acid, Exp. Eye Res. 1988;47: 37–50.
  • Nagaraj RH, Prabhakaram M, Ortwerth BJ, Monnier VM. Suppression of pentosidine formation in galactosemic rat lens by an inhibitor of aldose reductase. Diabetes. 1994 ;43:580-6.
  • Zorić L. Parameters of oxidative stress in the lens, aqueous humor and blood in patients with diabetes and senile cataracts. Srp Arh Celok Lek. 2003 ;131:137-42.
  • Corresponding Address: Dr. Sinan EMRE
  • Inonu University Medical Faculty,
  • Turgut Ozal Medical Center, Research Hospital,
  • Ophthalmology Department
  • TR-44280 Malatya, Turkey Tel : 422 341 06 60 Fax : 422 341 0728
  • E-Mail: emre@inonu.edu.tr & mdsinanemre@yahoo.com

Diyabet Hastalarının Lens Ön Kapsüllerinde Glutatyon Seviyesi ve Glutatyon Peroksidaz Aktivitesi

Year 2008, Volume: 15 Issue: 3, 139 - 143, 01.06.2008

Sinan Emre Burhan Ateş Ahmet Taşar Selim Doğanay

Abstract

Amaç: Bu çalışmada yaşa bağlı kataraktı bulunan hastalar ile sıkı kontrol altındaki diabet hastalarının lens ön kapsüllerinde ölçülen glutatyon (GSH) seviyesi ve glutatyon peroksidaz (GSH-Px) aktiviteleri değerlendirilmiştir. Yöntem: Çalışmaya tip II diabeti ve kataraktı bulunan hastalar ile yaş uyumlu olacak şekilde yaşa bağlı kataraktı bulunan hastalar dahil edildiler. Çalışma kapsamında sıkı diabet kontrolünde olan 18 hasta ile (Grup A), yaşa bağlı kataraktı bulunan 26 hastanın (Grup B) fakoemulsifikasyon yöntemi ile yapılan katarakt operasyonlarında alınan lens ön kapsülleri dahil edilmiştir. Örnekler çözünüp, santrifüje edilmelerinden sonra numunelerden GSH seviyeleri ve GSH-Px enzim aktiviteleri ölçülmüştür. Sonuçlar: Grup A ortalama yaşları 60.3 ± 8.3 yıl olan, 10 bayan ve 8 erkek hastadan oluşmaktaydı. Grup B ise ortalama yaşları 63.9 ± 12.2 yıl olan, 11 bayan ve 15 erkek hastadan oluşmaktaydı. Grup A ve B için lens ön kapsülünde ölçülen ortalama GSH seviyeleri sırasıyla 82.4±16.5 ve 97.5±12.5 nmol/mg iken ortalama GSH-Px aktivite düzeyleri sırasıyla 27.0±9.7 ve 36.9±9.3 mmol/mg protein idi. Her iki parametre içinde gruplar arasındaki fark istatistiksel olarak anlamlı düzeyde tespit edilmedi (p>0.05). Yorum: Diabetin anti-oksidan mekanizmalar üzerindeki zararlı etkisi iyi bilinmektedir. Bizim sonuçlarımızda GSH seviyesinin ve GSH-Px aktivitelerinin sıkı kontrollü diabet grubunda ve yaşa bağlı kataraktı grubu arasında yakın değerlerde çıkmış olması, diabetin sıkı kontrolünün diabetin lens üzerindeki etkilerini hafifletmek bakımından önemli olduğunu düşündürtmektedir. Anahtar kelimeler: Katarakt, Diabetes mellitus, Glutation (GSH), Glutation peroksidaz (GSH- Px), Sıkı kontrollü diabet

Keywords

Katarakt Diabetes mellitus Glutation (GSH) Glutation peroksidaz (GSH- Px) Sıkı kontrollü diabet

References

  • Özmen B, Özmen B, Erkin E, et al. Lens superoxide dismutase and catalase activities in diabetic cataract. Clinical Biochemistry 2002; 35: 69-72.
  • Godin DV, Wohaieb SA, Garnett ME, Goumeniouk AD. Antioxidant enzyme alterations in experimental and clinical diabetes. Mol Cell Biochem. 1988 ; 84:223- 31.
  • Mileva M, Bakalova R, Zlateva G. Low-Intensity Laser Irradiation Does not Affect the Oxidative Stress in Experimental Cataract. Med. Laser Appl. 2004; 19:150-4.
  • Obara Y. The oxidative stress in the cataract formation. Nippon Ganka Gakkai Zasshi. 1995 ; 99:1303-41.
  • Ganea E, Harding JJ. Glutathione-related enzymes and the eye. Curr Eye Res. 2006;31:1-11.
  • Harding JJ. Free and protein-bound glutathione in normal and cataractous human lenses. Biochem J. 1970 ;117:957-60.
  • George S, Jyothi M, Mathew B, Shashidhar S. Changes in glutathione, glutathione- linked enzymes and hexose monophosphate shunt enzymes in senile cataract. Indian J Physiol Pharmacol. 2003 ;47:191-6.
  • Donma O, Yorulmaz E, Pekel H, Suyugül N. Blood and lens lipid peroxidation and antioxidant status in normal individuals, senile and diabetic cataractous patients. Curr Eye Res. 2002 ;25:9-16.
  • Doganay S, Borazan M, Iraz M, Cigremis Y. The effect of resveratrol in experimental cataract model formed by sodium selenite. Curr Eye Res. 2006 ;31:147-53.
  • Doganay S, Turkoz Y, Evereklioglu C, et al. Use of caffeic acid phenethyl ester to prevent sodium-selenite-induced cataract in rat eyes. J Cataract Refract Surg. 2002 ;28:1457-62.
  • Stitt AW. Advanced glycation: an important pathological event in diabetic and age related ocular disease. Br J Ophthalmol. 2001 ;85:746-53.
  • Blakytny R, Harding JJ. Glycation (non-enzymic glycosylation) inactivates glutathione reductase. Biochem J. 1992 ;288 :303-7.
  • Ortwerth BJ, Olesen PR. Glutathione inhibits the glycation and crosslinking of lens proteins by ascorbic acid, Exp. Eye Res. 1988;47: 37–50.
  • Nagaraj RH, Prabhakaram M, Ortwerth BJ, Monnier VM. Suppression of pentosidine formation in galactosemic rat lens by an inhibitor of aldose reductase. Diabetes. 1994 ;43:580-6.
  • Zorić L. Parameters of oxidative stress in the lens, aqueous humor and blood in patients with diabetes and senile cataracts. Srp Arh Celok Lek. 2003 ;131:137-42.
  • Corresponding Address: Dr. Sinan EMRE
  • Inonu University Medical Faculty,
  • Turgut Ozal Medical Center, Research Hospital,
  • Ophthalmology Department
  • TR-44280 Malatya, Turkey Tel : 422 341 06 60 Fax : 422 341 0728
  • E-Mail: emre@inonu.edu.tr & mdsinanemre@yahoo.com
There are 21 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Sinan Emre This is me

Burhan Ateş This is me

Ahmet Taşar This is me

Selim Doğanay This is me

Publication Date June 1, 2008
Published in Issue Year 2008 Volume: 15 Issue: 3

Cite

APA Emre, S., Ateş, B., Taşar, A., Doğanay, S. (2008). Diyabet Hastalarının Lens Ön Kapsüllerinde Glutatyon Seviyesi ve Glutatyon Peroksidaz Aktivitesi. Journal of Turgut Ozal Medical Center, 15(3), 139-143.
AMA Emre S, Ateş B, Taşar A, Doğanay S. Diyabet Hastalarının Lens Ön Kapsüllerinde Glutatyon Seviyesi ve Glutatyon Peroksidaz Aktivitesi. J Turgut Ozal Med Cent. June 2008;15(3):139-143.
Chicago Emre, Sinan, Burhan Ateş, Ahmet Taşar, and Selim Doğanay. “Diyabet Hastalarının Lens Ön Kapsüllerinde Glutatyon Seviyesi Ve Glutatyon Peroksidaz Aktivitesi”. Journal of Turgut Ozal Medical Center 15, no. 3 (June 2008): 139-43.
EndNote Emre S, Ateş B, Taşar A, Doğanay S (June 1, 2008) Diyabet Hastalarının Lens Ön Kapsüllerinde Glutatyon Seviyesi ve Glutatyon Peroksidaz Aktivitesi. Journal of Turgut Ozal Medical Center 15 3 139–143.
IEEE S. Emre, B. Ateş, A. Taşar, and S. Doğanay, “Diyabet Hastalarının Lens Ön Kapsüllerinde Glutatyon Seviyesi ve Glutatyon Peroksidaz Aktivitesi”, J Turgut Ozal Med Cent, vol. 15, no. 3, pp. 139–143, 2008.
ISNAD Emre, Sinan et al. “Diyabet Hastalarının Lens Ön Kapsüllerinde Glutatyon Seviyesi Ve Glutatyon Peroksidaz Aktivitesi”. Journal of Turgut Ozal Medical Center 15/3 (June 2008), 139-143.
JAMA Emre S, Ateş B, Taşar A, Doğanay S. Diyabet Hastalarının Lens Ön Kapsüllerinde Glutatyon Seviyesi ve Glutatyon Peroksidaz Aktivitesi. J Turgut Ozal Med Cent. 2008;15:139–143.
MLA Emre, Sinan et al. “Diyabet Hastalarının Lens Ön Kapsüllerinde Glutatyon Seviyesi Ve Glutatyon Peroksidaz Aktivitesi”. Journal of Turgut Ozal Medical Center, vol. 15, no. 3, 2008, pp. 139-43.
Vancouver Emre S, Ateş B, Taşar A, Doğanay S. Diyabet Hastalarının Lens Ön Kapsüllerinde Glutatyon Seviyesi ve Glutatyon Peroksidaz Aktivitesi. J Turgut Ozal Med Cent. 2008;15(3):139-43.