Hipospadias Hastalarının Sünnet Derisinde Epidermal Büyüme Faktörü Reseptörünün Ekspresyonu

Year 2015, Volume: 22 Issue: 2, 81 - 85, 09.12.2015

Necla Gürbüz Sema Uğuralp Feral Öztürk Hale Kırımlıoğlu

Abstract

Amaç: Hipospadiasın etyolojisi genellikle belirsiz ve multifaktöriyeldir. Epidermal Büyüme Faktörü (EGF, Epidermal Growth Factor) akciğer
ve barsak matürasyonunu hızlandırır, hücre proliferasyonunu kolaylaştırır, doku farklılaşmasını ve onkojenezi düzenler, epitel yüzeylerinin
normal rejenerasyonu ve iyileşmesinde çok önemli rol oynar. Periüretral deride yetersiz EGF ekspresyonu bildirilmiştir.
Çalışmamızın amacı hipospadiaslı çocukların sünnet derisindeki ve sünnet olan çocukların sünnet derisindeki EGFR ekspresyonlarını
karşılaştırmaktı.
Gereç ve Yöntem: Yedi sağlıklı çocuğun (sünnet sırasında alınmıştır) ve 7 hipospadiaslı çocuğun sünnet derileri epidermal büyüme faktörü
reseptörü (EGFR) ekspresyonunun tespiti için boyanmıştır.
Bulgular: Gruplar arasında sünnet derilerinin epidermisinde, düz kasında, stromasında, kan damarlarında ve fibroblastlarındaki EGFR
ekspresyonu açısından anlamlı fark yoktu (p>0.05). Ancak hipospadias grubunda mast hücrelerinin sayısı artmıştı (p

Keywords

Hipospadias, EGF, EGFR, Mast Hücresi

Epidermal Growth Factor Receptor Expression in the Foreskin of Hypospadias Patients

Abstract

Objectives: The etiology of hypospadias is often obscured and multifactorial. Epidermal growth factor (EGF) accelerates lung and intestinal maturation, promotes cell proliferation, regulates tissue differentiation, and modulates organogenesis and it plays an essential role in the normal regeneration and healing of epithelial surfaces. Inadequate EGFR expression has been reported in periüretral skin.

The aim of our study is to compare the EGFR expression in the dorsal foreskin of children with hypospadias and in foreskin of children undergoing circumcision.

Materials and Methods: The foreskins of 7 healthy children (obtained during circumcision) and 7 children with hypospadias were stained for epidermal growth factor receptor (EGFR) expression.

Results: The EGFR expression in the epidermis, smooth muscle, stroma, blood vessels, and fibroblasts of prepuces were not significantly different between the groups (p>0,05). But the number of the mast cells were increased in the hypospadias group (p<0,05).

Discussion: EGFR expression did not differ between the dorsal foreskins of children with hypospadias and foreskins of children undergoing circumcision. But the numbers of mast cells were increased in the foreskins of children with hypospadias. Further cellular studies are required to uncover the mechanism underlying the increase in mast cell number.

Keywords

Hypospadias, EGF, EGFR, Mast Cell

References

• Dolk H, Vrijheid M, Scott JE, Addor MC, Botting B, de Vigan C, et al. Toward the effective surveillance of hypospadias. Environ Health Perspect 2004; 112:398-402.
• Paulozzi LJ, Erickson JD, Jackson RJ. Hypospadias trends in two U.S. surveillance systems. Pediatrics 1997;100 :831-34.
• Van der Zanden LF, Van Rooij IA, Feitz WF, Franke B, Knoers NV, Roeleveld N. Aetiology of hypospadias: a systematic review of genes and environment. Hum Reprod Update. 2012;18:260-83.
• Albers N, Ulrichs C, Gliier S, Hiort O, Gernot HG, Sinnecker GHG, et al. Etiologic classification of severe hypospadias: implications for prognosis and management. J Pediatr 1997;13:386-92.
• Aarskog D. Maternal progestins as a possible cause of hypospadias. N Engl J Med 1979;300:75-8.
• Gill GN, Bertics PJ, Santon JB. Epidermal growth factor and its receptor. Mol Cell Endocrinol 1987;51:86-96.
• Vinter-Jensen L. Pharmacological effects of epidermal growth factor (EGF) with focus on the urinary and gastrointestinal tracts. APMIS Suppl 1999;93:1-42.
• Barrow RE, Wang CZ, Evans MJ, Herndon DN. Growth factors accelerate epithelial repair in sheep trachea. J Lung 1993;171;335-44.
• Uguralp S, Akin M, Karabulut A Bay, Harma B, Kiziltay A, TR Kıran, et al. Reduction of peritoneal adhesions by sustained and local administration of epidermal growth factor. Pediatr Surg International 2008;24:191-97.
• Hong JP, Kim YW, Jung HD, Jung KI. The effect of various concentrations of human recombinant epidermal growth factor on split-thickness skin wounds. Int Wound J 2006;3:123-30.
• Repertinger SK, Campagnaro E, Fuhrman J, El-Abaseri T, Yuspa SH, Hansen LA. EGFR enhances early healing after cutaneous incisional wounding. J Invest Dermatol 2004;123:982-89.
• Levine E, Cupp AS, Miyashiro L, Skinner MK. Role of transforming growth factor-alpha and the epidermal growth factor receptor in embryonic rat testis development. Biol Reprod 2000;62:477-90.
• Cupp AS, Skinner MK. Expression, action, and regulation of transforming growth factor alpha and epidermal growth factor receptor during embryonic and perinatal rat testis development. J Androl 2001;22:1019-29.
• Kassab M, Abd-Elmaksoud A, Ali MA. Localization of the epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in the bovine testis. J Mol Histol 2007;38:207-214.
• Baskin LS, Himes K, Colborn T. Hypospadias and Endocrine Disruption: Is there a connection? Enviromental health perspectives 2001;11:1175-83.
• Yang XF, Han XB, Rong DW, Li DB. Impact of the hCG on epidermal growth factor in the phallic tissue of hypospadias mice. Zhonghua Nan Ke Xue 2007;13:1072-74.
• Gupta C, Jaumotto J. Epidermal growth factor binding in the developing male reproductive duct and its regulation by testosterone. Endocrinology 1993;133:1778-82.
• Bai S, Wilson EM. Epidermal growth factor-dependent phosphorylation and ubiquitinylation of MAGE- 11 regulates its interaction with the androgen receptor. Moll Cell Biol 2008;28:1947-63.
• El-galley RES, Cohen ESC, Petros JA, Woodard J, Galloway NTM. Epidermal growth factor (EGF) and EGF receptor in hypospadias. British Journal of Urology 1997;79:116-19.
• 2. Metcalfe DD, Baram D, Mekori YA. Mast cells. Physiol Rev 1997;77:1033-79.
• Meininger CJ, Zetter BR. Mast cell and angiogenesis. Semin Cancer Biol 1992;3:73-9.
• Qu Z, Liebler JM, Powers MR, Galey T, Ahmadi P, Huang XN, et al. Mast cells are a major source of basic fibroblast growth factor in chronic inflammation and cutaneous hemangioma. Am J Pathol 1995;147:564-73.
• Toda S, Sugihara H. Reconstruction of thyroid follicles from isolated porcine follicle cells in three-dimensional collagen gel culture. Endocrinology 1990; 126:2027-34.
• Hermanowicz A, Debek W, Dzienis-Koronkiewicz E, Chyczewski L. Topography and morphometry of intestinal mast cells in children with Hirschsprung’s disease. Folia Histochem Cytobiol. 2008;46:65-8.