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Apo E Genotyping İn Tuberculosis Patients From Malatya

Year 2015, Volume: 22 Issue: 3, 180 - 183, 14.12.2015

Ceren Acar Aslı Giray-kurt R.Dilara Vaizoğlu G.Harika Gözükara Zeynep Arıca Bekir Durmaz Üner Kayabaş Barış Otlu

Abstract

Objectives: The aim of this study is to genotype the Apo E polymorphism and to show the variations in our tuberculosis patients and healthy volunteers who are from an area which has a high tuberculosis prevalance in Turkey. By comparing the data from patients and healthy individuals we aimed to understand whether host related factors like Apo E genotype of the patients are taking a role in the development of infection.

Material and Methods: In this study 60 Tuberculosis (TB) patients and 160 healthy individuals collected by Turgut Ozal Medical Center and Malatya Public Health Office were included. DNA was isolated from peripheral blood samples. Apo E genotyping was performed by using a commercial Real-Time PCR kit. The Fisher’s exact test and Likelihood Ratio test was used to compare genotype and allele frequencies.

Results: Unlike others our study revealed no significant association between tuberculosis and Apo E genotypes. The data was analysed by comparing patient and healthy subject results.

Conclusion: This is the first study from Turkey that focuses on the association of Apo E and tuberculosis infection. Although others found, we couldn’t find an association between tuberculosis and Apo E genotypes in our subject group. Although all the patients that could be reached in Malatya were included, the main limitation was the number of patients. By looking at bigger populations we may have more informative data.

Key Words: TB; SNP; Apo E; Genotyping.

Keywords

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References

  • Gunal S, Yang Z, Agarwal M, Koroglu M, Arızı Z K, Durmaz R. Demographic and microbial characteristics of extrapulmonary tuberculosis cases diagnosed in Malatya, Turkey, 2001-2007. BMC Public Health 2011;8:11:154.
  • Ma X, Liu Y, Gowen BB, Graviss EA, Clark AG, Musser JM. Full-exon resequencing reveals toll-like receptor variants contribute to human susceptibility to tuberculosis disease. PLoS One 2007;19:2(12):e1318.
  • Martens GW, Arikan MC, Lee J, Ren F, Vallerskog T, Kornfeld H. Hypercholesterolemia impairs immunity to tuberculosis. Infect Immun 2008;76(8):3464-72.
  • Otlu B, Durmaz R, Gunal S, Sola C, Zozio T, Rastogi N. Beijing/W and major spoligotype families of Mycobacterium tuberculosis strains isolated from tuberculosis patients in Eastern Turkey. New Micribiol 2009;32(3):255-63.
  • Agarwal M, Gunal S, Durmaz R, Yang Z. Integration of Mycobacterium tuberculosis drug susceptibility testing and genotyping with epidemiological data analysis to gain insight into the epidemiology of drug-resistant tuberculosis in Malatya, Turkey. J Clin Microbiol 2010;48(9):3301-5.
  • Alieke GV, de Bont N, Netea MG, Demacker PN, van der Meer JW, Stalenhoef AF et al. Apolipoprotein–E-deficient mice exhibit an increased susceptibility to disseminated candidiasis. Med Myecol 2004; 42:341-8.
  • de Bont N, Netea MG, Demacker PN, Kullberg BJ, van der Meer JW, Stalenhoef AF. Apolipoprotein–E-deficient mice have an impaired immune response to Klebsiella pneumonia. Eur J Clin Investig 2000;30:818-22.
  • Roselaar SE, and Daugherty A. Apolipoprotein–E-deficient mice have an impaired innate immune responses to Listeria monocytogenes in vivo. J Lipid Res 1998; 39:1740-3.
  • Minihane AM, Jofre-Monseny L, Olano-Martin E, Rimbach G. Apo E genotype, cardiovascular risk and responsiveness to dietary fat manipulation . Proc Nutr Soc 2007 66(2):183-97.
  • Strickland DK, Gonias SL, Argraves WS. Diverse roles for the LDL receptor family,Trends Endocrinol Metab. 2002 13(2):66-74.
  • Farivar TN, Moud BS, Sargazi M, Moeenrezakhanlou A. Apolipoprotein E Polymorphism in Tuberculosis Patients. J Appl Sci 2008;8(4):719-22.
  • Wozniak MA, Maude RJ, Innes JA, Hawkey PM, Itzhaki RF. Apolipoprotein E-epsilon2 confers risk of pulmonary tuberculosis in woman from the Indian subcontinent-a preliminary study . J Infect 2009; 59(3):219-22.
  • Gregor MF and Hotamisligil GS. Inflammatory mechanisms in obesity. Annu Rev Immunol 2011; 29:415-45.
  • Van Kerkhove MD, Vandemaele KAH, Shinde V, Jaramillo Gutierez G, KoukounariA, Donnely CA et al. Risk factors for severe outcomes following 2009 influenza (H1N1) infection : a global pooled analysis. PLoS Med 2011;8:e1001053.
  • Dooley KE, Chaisson RE. Tuberculosis and diabetes mellitus: convergence of two epidemics. Lancet Infect Dis 2009;737-46.
  • Brown MS, Goldstein JL. Koch’s postulates for cholesterol. Cell 1992;71:187-8.

Ceren Acar1, Aslı Giray-Kurt1, R.Dilara Vaizoğlu1, G.Harika Gözükara2, Zeynep Arıca3, Bekir Durmaz3, Üner Kayabaş4, Barış Otlu5

Year 2015, Volume: 22 Issue: 3, 180 - 183, 14.12.2015

Ceren Acar Aslı Giray-kurt R.Dilara Vaizoğlu G.Harika Gözükara Zeynep Arıca Bekir Durmaz Üner Kayabaş Barış Otlu

Abstract

Amaç: Bu çalışmadaki amacımız, ülke geneline göre tüberkülozlu hasta prevelansı yüksek olan bölgemizde, tüberküloz tanısı konmuş hasta populasyonunda ve sağlıklı gönüllülerde Apo E polimorfizmlerini genotiplendirmek ve varyasyonları göstermektir. Bu veriler ve normal bireylere ait veriler karşılaştırılarak olası Apo E-tübeküloz bağlantısına yönelik bir profil çıkarılması hedeflenmektedir. Gereç ve Yöntem: Çalışmaya Turgut Özal Tıp Merkezi Mikrobiyoloji Anabilim Dalı ve Malatya İl Sağlık Müdürlüğü tarafından toplanan 60 tüberkülozlu (TB) hasta ve 160 sağlıklı birey dahil edildi. Periferal kan örneklerinden DNA izole edildi. Apo E genotiplendirmesi, Real-Time PCR temelli hazır kit kullanılarak gerçekleştirildi. Genotip ve allel frekanslarını karşılaştırmak için Fisher kesin ki-kare testi ve olabilirlik oranı kullanılmıştır. Bulgular: Önceki çalışmaların aksine bizim çalışmamıza dahil ettiğimiz populasyonda tüberküloz ve Apo E genotipleri arasında bir bağlantı bulunamamıştır. Veriler hasta ve normallerin karşılaştırılması ve hasta ve sağlıklı bireylerin cinsiyetleri arasındaki farklılıkların karşılaştırılması ile analiz edilmiştir ve istatistiki açıdan anlamlı bir sonuca ulaşılamamıştır. Sonuç: Bu çalışma, Apo E genotipleri ve tüberküloz arasındaki bağlantıyı araştıran Türkiye’de yapılmış ilk çalışmadır. Diğer populasyonlarda bağlantı bulunmuş olmasına rağmen biz tüberküloz ve Apo E allelleri ve genotipleri arasında bir bağlantı bulunamamıştır. Çalışmaya, Malatya ilinde ulaşılabilen tüm hastalar dahil edilmiş olmasına rağmen kısıtlayıcı etkenlerden bir tanesi hasta sayısının az olmasıdır. Daha büyük örnek grubu ile çalışarak daha bilgilendirici bir sonuca ulaşılabileceği düşünülmektedir

Keywords

TB SNP Apo E Genotipleme

References

  • Gunal S, Yang Z, Agarwal M, Koroglu M, Arızı Z K, Durmaz R. Demographic and microbial characteristics of extrapulmonary tuberculosis cases diagnosed in Malatya, Turkey, 2001-2007. BMC Public Health 2011;8:11:154.
  • Ma X, Liu Y, Gowen BB, Graviss EA, Clark AG, Musser JM. Full-exon resequencing reveals toll-like receptor variants contribute to human susceptibility to tuberculosis disease. PLoS One 2007;19:2(12):e1318.
  • Martens GW, Arikan MC, Lee J, Ren F, Vallerskog T, Kornfeld H. Hypercholesterolemia impairs immunity to tuberculosis. Infect Immun 2008;76(8):3464-72.
  • Otlu B, Durmaz R, Gunal S, Sola C, Zozio T, Rastogi N. Beijing/W and major spoligotype families of Mycobacterium tuberculosis strains isolated from tuberculosis patients in Eastern Turkey. New Micribiol 2009;32(3):255-63.
  • Agarwal M, Gunal S, Durmaz R, Yang Z. Integration of Mycobacterium tuberculosis drug susceptibility testing and genotyping with epidemiological data analysis to gain insight into the epidemiology of drug-resistant tuberculosis in Malatya, Turkey. J Clin Microbiol 2010;48(9):3301-5.
  • Alieke GV, de Bont N, Netea MG, Demacker PN, van der Meer JW, Stalenhoef AF et al. Apolipoprotein–E-deficient mice exhibit an increased susceptibility to disseminated candidiasis. Med Myecol 2004; 42:341-8.
  • de Bont N, Netea MG, Demacker PN, Kullberg BJ, van der Meer JW, Stalenhoef AF. Apolipoprotein–E-deficient mice have an impaired immune response to Klebsiella pneumonia. Eur J Clin Investig 2000;30:818-22.
  • Roselaar SE, and Daugherty A. Apolipoprotein–E-deficient mice have an impaired innate immune responses to Listeria monocytogenes in vivo. J Lipid Res 1998; 39:1740-3.
  • Minihane AM, Jofre-Monseny L, Olano-Martin E, Rimbach G. Apo E genotype, cardiovascular risk and responsiveness to dietary fat manipulation . Proc Nutr Soc 2007 66(2):183-97.
  • Strickland DK, Gonias SL, Argraves WS. Diverse roles for the LDL receptor family,Trends Endocrinol Metab. 2002 13(2):66-74.
  • Farivar TN, Moud BS, Sargazi M, Moeenrezakhanlou A. Apolipoprotein E Polymorphism in Tuberculosis Patients. J Appl Sci 2008;8(4):719-22.
  • Wozniak MA, Maude RJ, Innes JA, Hawkey PM, Itzhaki RF. Apolipoprotein E-epsilon2 confers risk of pulmonary tuberculosis in woman from the Indian subcontinent-a preliminary study . J Infect 2009; 59(3):219-22.
  • Gregor MF and Hotamisligil GS. Inflammatory mechanisms in obesity. Annu Rev Immunol 2011; 29:415-45.
  • Van Kerkhove MD, Vandemaele KAH, Shinde V, Jaramillo Gutierez G, KoukounariA, Donnely CA et al. Risk factors for severe outcomes following 2009 influenza (H1N1) infection : a global pooled analysis. PLoS Med 2011;8:e1001053.
  • Dooley KE, Chaisson RE. Tuberculosis and diabetes mellitus: convergence of two epidemics. Lancet Infect Dis 2009;737-46.
  • Brown MS, Goldstein JL. Koch’s postulates for cholesterol. Cell 1992;71:187-8.
There are 16 citations in total.

Details

Primary Language Tr
Journal Section Articles
Authors

Ceren Acar This is me

Aslı Giray-kurt This is me

R.Dilara Vaizoğlu This is me

G.Harika Gözükara This is me

Zeynep Arıca This is me

Bekir Durmaz This is me

Üner Kayabaş This is me

Barış Otlu This is me

Publication Date December 14, 2015
Published in Issue Year 2015 Volume: 22 Issue: 3

Cite

APA Acar, C., Giray-kurt, A., Vaizoğlu, R., Gözükara, G., et al. (2015). Apo E Genotyping İn Tuberculosis Patients From Malatya. Journal of Turgut Ozal Medical Center, 22(3), 180-183.
AMA Acar C, Giray-kurt A, Vaizoğlu R, Gözükara G, Arıca Z, Durmaz B, Kayabaş Ü, Otlu B. Apo E Genotyping İn Tuberculosis Patients From Malatya. J Turgut Ozal Med Cent. December 2015;22(3):180-183.
Chicago Acar, Ceren, Aslı Giray-kurt, R.Dilara Vaizoğlu, G.Harika Gözükara, Zeynep Arıca, Bekir Durmaz, Üner Kayabaş, and Barış Otlu. “Apo E Genotyping İn Tuberculosis Patients From Malatya”. Journal of Turgut Ozal Medical Center 22, no. 3 (December 2015): 180-83.
EndNote Acar C, Giray-kurt A, Vaizoğlu R, Gözükara G, Arıca Z, Durmaz B, Kayabaş Ü, Otlu B (December 1, 2015) Apo E Genotyping İn Tuberculosis Patients From Malatya. Journal of Turgut Ozal Medical Center 22 3 180–183.
IEEE C. Acar, A. Giray-kurt, R. Vaizoğlu, G. Gözükara, Z. Arıca, B. Durmaz, Ü. Kayabaş, and B. Otlu, “Apo E Genotyping İn Tuberculosis Patients From Malatya”, J Turgut Ozal Med Cent, vol. 22, no. 3, pp. 180–183, 2015.
ISNAD Acar, Ceren et al. “Apo E Genotyping İn Tuberculosis Patients From Malatya”. Journal of Turgut Ozal Medical Center 22/3 (December 2015), 180-183.
JAMA Acar C, Giray-kurt A, Vaizoğlu R, Gözükara G, Arıca Z, Durmaz B, Kayabaş Ü, Otlu B. Apo E Genotyping İn Tuberculosis Patients From Malatya. J Turgut Ozal Med Cent. 2015;22:180–183.
MLA Acar, Ceren et al. “Apo E Genotyping İn Tuberculosis Patients From Malatya”. Journal of Turgut Ozal Medical Center, vol. 22, no. 3, 2015, pp. 180-3.
Vancouver Acar C, Giray-kurt A, Vaizoğlu R, Gözükara G, Arıca Z, Durmaz B, Kayabaş Ü, Otlu B. Apo E Genotyping İn Tuberculosis Patients From Malatya. J Turgut Ozal Med Cent. 2015;22(3):180-3.
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