SONBAHAR MEVSİMİNDE İKİ ÖRNEKLEME METODU KULLANARAK EDİRNE İLİNİN DOĞUSUNDA HAVAYLA TAŞINAN FUNGAL KONSANTRASYONLARIN BELİRLENMESİ

Abstract

Gece ve gündüz saatlerinde fungal konsantrasyonlar değişiklik gösterebilir. Kuru sporlar havaya gündüz salınırken nem oranı daha yüksek olan sporlar gece salınmaktadırlar. Türkiye’de havayla taşınan funguslar ile ilgili çalışmalar çok azdır ve yalnızca bazı şehirlerde günlük ve haftalık incelemeler yapılmıştır. Bu çalışmada Edirne şehri günlük fungal konsantrasyonlarının belirlenmesi amaçlanmıştır. Örnekler yerden 11 m yükseklikten alınmıştır. Örneklemeler 11 Ekim 2002 – 15 Kasım 2002 tarihleri arasında, hergün aynı saatlerde olmak üzere birer haftalık zaman dilimlerinde sabah 08.00 ve akşam 20.00’de yapılmıştır. Fungus izolasyonu için yerçekimine dayalı petri plak metodu ve volumetrik örnekleme metodu kullanılmıştır. Sıcaklık ve nısbi nem iklimsel faktörlerin fungal sporları etkileyip etkilemedikleri istatistiki olarak incelenmiş ve fungus sayıları, nısbi nem ve sıcaklık arasında korelasyon bulunmuştur. 42 petri plağına izole edilen toplam 800 fungus kolonisi bulunma sıklıkları belirlenerek cins düzeyinde tanımlamaları yapılmıştır. Volumetrik metot ile m3 de 9337 cfu mikrofungus belirlenmiştir. En baskınları Alternaria, Penicillium ve Trichoderma olan 7 fungus cinsi teşhis edilebilmiştir.

Keywords

• Havayla taşınan funguslar, fungal konsantrasyon değişiklikleri, volumetrik metod

AIRBORNE FUNGAL CONCENTRATIONS IN EAST PATCH OF EDIRNE CITY (TURKEY) IN AUTUMN USING TWO SAMPLING METHODS

Abstract

Fungal spore concentrations vary during day and night. Dry spore types are released during the day, whereas wet spores that have more humidity ratio are released into the air at night. Studies on airborne fungi in Turkey are very rare, and they are daily and weekly monitored only in some cities. The aim of our study was to determine the daily fungal concentrations in Edirne, Turkey. All samples were taken from a level of 11 m. above ground. The sampling time was carried out bihourly in the day, from 8 am to 20 pm, once a week from October 11 to November 15, 2002. Two sampling methods were used for the isolation of fungi: A gravitational and a volumetric method. Statistical analysis was performed in order to determine the relationship between some environmental factors and airborne counts and some correlation were found between the fungal numbers, relative humidity, sampling hours and temperature. A total of 800 fungal colonies in 42 petri dishes were isolated, quantified to determine the frequency of occurrence and then identified as genus level. 9337 cfu/m3 microfungi were determined by volumetric sampling. Seven fungal genera could be determined, among them Cladosporium and Alternaria were found as the most predominant fungi, followed by Penicillium and Trichoderma.

Keywords

• Airborne fungi, airspora, biomass, diurnal differences, gravitational sampling volumetric sampling

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