Karyometric analysis of nasal polyps

Year 2006, Volume: 16 Issue: 6, 251 - 254, 02.12.2006

Altan Yıldırım Öztürk Özdemir Eylem Gül Beyazıt Yıldırım Şahende Elagöz Tanfer Kunt

Abstract

Objectives: Nuclear characteristics of epithelial cells in nasal polyps were analyzed.Patients and Methods: The slides of 35 patients who undervvent surgery for nasal polyposis and 18 slides of normal mucosa were examined. The specimens were stained with hematoxylineosin. Tissue sections were analyzed by a CCD camera and the Karyotype Program of Human Cytogenetics Nomenclature System on a Macintosh Computer OS 9 . Fifty epithe­lial cells in each slide were randomly selected and transferred to the automated karyotyping system and photographed. The diameters of the nuclei were mea- sured and some peculiar nuclear features were exam- ined, including unsmooth appearance of the nuclear membrane, anaphase-bridge, and binucleation.Results: There were significant differences between nasal polyp and normal mucosa groups in terms of nuclear diameter, perimeter, and volüme, with ali variables being greater in the nasal polyp group p=0.001 . Unusual nuclear features differed significantly unsmooth nuclear membrane appearance, p<0.001; anaphase-bridge, p=0.041; binucleation, p=0.018 , as well.Conclusion: Epithelial cells of nasal polyps exhibit nuclear instability.

Keywords

Karyometry, nasal polyp

Nazal poliplerin karyometrik incelemesi

Abstract

Amaç: Bu çalışmada nazal polip epitel hücrelerininçekirdek yapıları incelendi.Hastalar ve Yöntemler: Nazal polipozis nedeniyleameliyat edilen 35 hastanın kesitleri ve 18 normalmukoza kesiti çalışıldı. Kesitler hematoksilen-eozinile boyandı. Doku bölümleri CCD kamera ve MacOS 9 Karyotype Program of Human CytogeneticsNomenclature System yardımı ile incelendi. Her birkesitten 50 epitel hücresi rastgele seçildi ve otomatik karyotip sistemine aktarılarak fotoğraflandı. Çekirdek çapları ölçüldü. Ayrıca, sıra dışı çekirdek yapıları olan düzensiz çekirdek membranı, anafazköprüsü ve çift çekirdekli hücre oranları incelendi.Veriler nazal polip ve normal mukoza grupları arasında karşılaştırıldı.Bulgular: Nazal polip epitel hücresi çekirdeği ilenormal mukoza çekirdeği arasında çekirdek çapı,çevresi ve hacmi açısından anlamlı farklılık bulundu;bu değişkenlerin hepsi polip grubunda artış gösterdi p=0.001 . Sıra dışı çekirdek yapıları bakımından daiki grup arasında belirgin farklılık vardı düzensiz çekirdek membranı, p

Keywords

Karyometri, nazal polip

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