Kerevit Kabuklarından Elde Edilen Kitosanın Krem Formülasyonlarındaki Antimikrobiyal Etkileri

Year 2024, , 206 - 216, 01.12.2024

Yavuz Mazlum , Selin Sayın , Betül Aydın , Mehmet Naz , Metin Yazıcı , Şerife Akküçük

https://doi.org/10.52998/trjmms.1471661

Abstract

Bu çalışmanın amacı, kitosan bazlı bir kremin antimikrobiyal özelliklerinin araştırılmasıdır. Bunun için, kitosan ilave edilmiş bir kremin antimikrobiyal etkileri kontrol grubu ile karşılaştırılmıştır. Ana materyal olarak, çalışma kapsamında Eğirdir Gölü'nden dondurulmuş bir şekilde temin edilen kerevitlerden ekstrakte edilen kitosan kullanılmıştır. Çalışma, kontrol (F1) ve muamele (F2) grupları arasında bir karşılaştırmayı içermektedir. Her iki krem formülasyonu da bakteriyel inhibisyon sergilerken, yalnızca F1 formülasyonu C. albicans için canlı mikroorganizmalarda önemli bir azalma göstermiştir. Sitotoksisite değerlendirmesi, örneklerin sitotoksik etkilerinde konsantrasyona bağlı bir artış olduğunu ortaya koymuştur. Özellikle, F1 formülasyonunun sağlıklı hücreler üzerinde F2 formülasyonuna kıyasla daha yüksek bir toksisite sergilediği gözlenmiştir. Sonuç olarak, sitotoksik etkilerinin altında yatan mekanizmaları anlamak ve formülasyonlarını biyouyumluluğu artıracak şekilde optimize etmek için daha fazla araştırma yapılması gerekmektedir. Ayrıca, bu çalışmada geliştirilen kitosan bazlı krem, test edilen bakterilere karşı dikkate değer antimikrobiyal etkinlik göstermiştir.

Keywords

Kitosan, Kerevit, Antimikrobiyal etki, Sitotoksisite, Deri sağlığı

Project Number

This study was not supported by any organization. There is no project number.

Antimicrobial Effects of Chitosan Extracted from Crayfish Shells in Cream Formulations

Abstract

The objective of the present study was to investigate the antimicrobial properties of a chitosan-based cream. To achieve this, the antimicrobial effects of a cream enriched with chitosan were compared with those of a control group. Chitosan, sourced from crayfish obtained frozen from Eğirdir Lake, Eğirdir, Isparta, served as the primary material. The study involved a comparison between control (F1) and treatment (F2) groups. While both cream formulations exhibited bacterial inhibition, only the F1 formulation demonstrated a significant reduction in viable microorganisms for C. albicans. The cytotoxicity assessment revealed a concentration-dependent increase in the cytotoxic effects of the samples. Notably, the F1 formulation exhibited higher toxicity to healthy cells compared to the F2 formulation. In conclusion, further investigation is necessary to understand the mechanisms underlying their cytotoxic effects and to optimize their formulations to enhance biocompatibility. Moreover, the chitosan-based cream developed in this study demonstrated notable antimicrobial efficacy against the tested bacteria.

Keywords

Chitosan, Crayfish, Antimicrobial effect, Cytotoxicity, Skin health

Ethical Statement

Ethics committee permission is not required for this study.

Supporting Institution

This study was not supported by any organization

Project Number

This study was not supported by any organization. There is no project number.

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