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OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY

Year 2018, Volume: 19 Issue: 2, 159 - 173, 15.10.2018
https://doi.org/10.23902/trkjnat.439763

Abstract

Bu çalışmada, daha önce sünmüş ekmekten izole edilmiş
olan Bacillus amyloliquefaciens
FE-K1’in ekstraselüler peptidaz üretiminin, merkezi kompozit tasarıma (MKT)
dayalı yanıt yüzey yöntemi (YYY) ile optimize edilmesi amaçlanmıştır. Yanıt
yüzey yönteminde sıcaklık (20-45°C), enzim üretim ortamının başlangıç pH değeri
(pH 5-9) ve inokülasyon seviyesi (%1-5, v/v) faktör olarak kullanılmış,
fermentasyon süresi her deneme için ayrı ayrı belirlenmiştir. Sonuçlar optimum
peptidaz üretiminin 33,4°C, pH 6,62 ve %2,3 inokülasyon seviyesinde elde
edildiğini göstermiştir. Optimum koşullar altında fermentasyon süresinin sadece
7 saat, ham enzimin aktivitesinin 49,17U/mL, spesifik aktivitesinin ise
504,77U/mg olduğu tespit edilmiştir.

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OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY

Year 2018, Volume: 19 Issue: 2, 159 - 173, 15.10.2018
https://doi.org/10.23902/trkjnat.439763

Abstract

In this study, it was aimed to optimise the extracellular peptidase production of Bacillus amyloliquefaciens FE-K1, previously isolated from ropy wholemeal bread, by using response surface methodology (RSM) based on central composite design (CCD). The temperature (20-45°C), initial pH of the enzyme production medium (pH 5-9) and inoculation level (1-5%, v/v) were used as the factors for RSM, and the fermentation time was determined for each trial separately. Results showed that the optimum peptidase production occurred at 33.4°C, pH 6.62 and 2.3% inoculation. It was determined that the fermentation time was only 7h, the crude enzyme had a peptidase activity of 49.17U/mL and a specific activity of 504.77U/mg under the optimised conditions.

References

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  • 25. Hammami, A., Bayoudh, A., Abdelhedi, O. & Nasri, M. 2018. Low-cost culture medium for the production of proteases by Bacillus mojavensis SA and their potential use for the preparation of antioxidant protein hydrolysate from meat sausage by-products. Annals of Microbiology, 68: 473-484.
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  • 27. Hanlon, G.W. & Hodges, N.A. 1981. Bacitracin and protease production in relation to sporulation during exponential growth of Bacillus licheniformis on poorly utilized carbon and nitrogen sources. Journal of Bacteriology, 147(2): 427-431.
  • 28. Harwood, C.R. & Cranenburgh, R. 2008. Bacillus protein secretion: an unfolding story. Trends in Microbiology, 16(2): 73-79.
  • 29. Hussain, F., Kamal, S., Rehman, S., Azeem, M., Bibi, I., Ahmed, T. & Iqbal, H.M.N. 2017. Alkaline protease production using response surface methodology, characterization and industrial exploitation of alkaline protease of Bacillus subtilis sp. Catalysis Letters, 147(5): 1204-1213.
  • 30. Jaswal, R.K., Kocher, G.S. & Virk, M.S. 2008. Production of alkaline protease by Bacillus circulans using agricultural residues: A statistical approach. Indian Journal of Biotechnology, 7: 356-360.
  • 31. Johnvesly, B. & Naik, G.R. 2001. Studies on production of thermostable alkaline protease from thermophilic and alkaliphilic Bacillus sp. JB-99 in a chemically defined medium. Process Biochemistry, 37: 139-144.
  • 32. Kim, M., Si, J.B., Reddy, L.V. & Wee, Y.J. 2016. Enhanced production of extracellular proteolytic enzyme excreted by a newly isolated Bacillus subtilis FBL-1 through combined utilization of statistical designs and response surface methodology. RSC Advances, 6: 51270-51278.
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  • 35. Kolkman, M., Mejldal, R., Goedegebuur, F., Babe, L.M., Kellett-Smith, A.H., Mulder, H., Bott, R.R. & Scotcher, M.C. 2016. Serine proteases of the Bacillus gibsonii-clade. US Patent No. US20160319266A1.
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There are 74 citations in total.

Details

Primary Language English
Journal Section Research Article/Araştırma Makalesi
Authors

Fundagül Erem 0000-0003-1562-0686

Mehmet İnan This is me 0000-0003-1806-7927

Muharrem Certel 0000-0002-1901-5590

Publication Date October 15, 2018
Submission Date July 2, 2018
Acceptance Date October 9, 2018
Published in Issue Year 2018 Volume: 19 Issue: 2

Cite

APA Erem, F., İnan, M., & Certel, M. (2018). OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY. Trakya University Journal of Natural Sciences, 19(2), 159-173. https://doi.org/10.23902/trkjnat.439763
AMA Erem F, İnan M, Certel M. OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY. Trakya Univ J Nat Sci. October 2018;19(2):159-173. doi:10.23902/trkjnat.439763
Chicago Erem, Fundagül, Mehmet İnan, and Muharrem Certel. “OPTIMISATION OF Bacillus Amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY”. Trakya University Journal of Natural Sciences 19, no. 2 (October 2018): 159-73. https://doi.org/10.23902/trkjnat.439763.
EndNote Erem F, İnan M, Certel M (October 1, 2018) OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY. Trakya University Journal of Natural Sciences 19 2 159–173.
IEEE F. Erem, M. İnan, and M. Certel, “OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY”, Trakya Univ J Nat Sci, vol. 19, no. 2, pp. 159–173, 2018, doi: 10.23902/trkjnat.439763.
ISNAD Erem, Fundagül et al. “OPTIMISATION OF Bacillus Amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY”. Trakya University Journal of Natural Sciences 19/2 (October 2018), 159-173. https://doi.org/10.23902/trkjnat.439763.
JAMA Erem F, İnan M, Certel M. OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY. Trakya Univ J Nat Sci. 2018;19:159–173.
MLA Erem, Fundagül et al. “OPTIMISATION OF Bacillus Amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY”. Trakya University Journal of Natural Sciences, vol. 19, no. 2, 2018, pp. 159-73, doi:10.23902/trkjnat.439763.
Vancouver Erem F, İnan M, Certel M. OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY. Trakya Univ J Nat Sci. 2018;19(2):159-73.

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