Rhizogenesis in Shrub rose cultivated in vitro

Larysa Koldar; Iryna Denysko; Alla Konopelko; Yevhen Mazur

doi:10.23902/trkjnat.1464147

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Rhizogenesis in Shrub rose cultivated in vitro

Year 2024, Volume: 25 Issue: 2, 187 - 196, 15.10.2024

Larysa Koldar Iryna Denysko Alla Konopelko , Yevhen Mazur

https://doi.org/10.23902/trkjnat.1464147

Abstract

The study of the reproduction characteristics of roses of the garden class Shrub, the defini-tion of the dependence of the hormonal determination of explant rhizogenesis on the con-centrations of phytohormones that are part of the nutrient medium, are relevant and has both scientific and practical interest. This study presents the results of studies of hormonal de-termination of rhizogenesis in explants of cultivars of roses of the garden class Shrub: Gärt-nerfreude, Lavender Dream, Pomponella, Red Cascade, Sommerabend cultivated in vitro on nutrient medium containing growth regulators. It has been established that of the nutrient medium modified by the addition of 0.2-1.0 mg/l α-naphthylacetic acid (α-NAA), the most effective was the medium with the content of α-NAA 0.5 mg/l, the content of macro- and microelements half of the Murashige and Skoog prescriptions, and a decrease in the sucrose content to 2.0%. On this medium, the frequency of rhizogenesis averaged 61.2% for the studied cultivars. Hormonal determination of rhizogenesis and efficiency of root formation in vitro in the Shrub rose regenerants depended on the genotype of the plant: cv. Lavender Dream (66.0%) and cv. Sommerabend (67.0%) had the highest rhizogenesis ability. The use of the universal growth regulator Humifield in combination with 0.5 mg/l α-NAA contribut-ed to an increase in the rooting rate of the studied rose cultivars up to 70.0-86.0%.

Keywords

micropropagation, roses, phytohormones, nutrient medium, determination, rooting

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Year 2024, Volume: 25 Issue: 2, 187 - 196, 15.10.2024

Larysa Koldar Iryna Denysko Alla Konopelko , Yevhen Mazur

https://doi.org/10.23902/trkjnat.1464147

Abstract

Çalı güllerinin üreme özelliklerinin incelenmesi ve eksplant kök oluşumunun hormonal kontrolünün kullanılan besleyici ortamın fitohormon konsantrasyonuna bağlı olduğunun tanımlanması önemlidir ve hem bilimsel hem de pratik açıdan üzerinde durulan konulardır. Bu çalışmada, büyüme düzenleyicileri içeren besin ortamı üzerinde in vitro olarak yetiştiri-len çalı gülü kültivarlarının (Gärtnerfreude, Lavender Dream, Pomponella, Red Cascade, Sommerabend) eksplantlarında kök oluşumunun hormonal belirlenmesine yönelik deneme-lerin sonuçları sunulmuştur. 0,2-1,0 mg/l a-naftilasetik asit (a-NAA) ilavesiyle modifiye edilen besin ortamlarından en etkilisinin, 0,5 mg/l a-NAA içerikli, sukroz oranı %2’ye kadar düşürülmüş ortam olduğu tespit edilmiştir. Bu ortamda, çalışılan kültivarlar için kök oluşu-mu sıklığı ortalama %61,2 olarak belirlenmiştir. Kullanılan kültivarlardaki kök oluşumunun in vitro etkinliğindeki hormonal katkının kültivarların genotiplerine bağlı olduğu belirlen-miştir: Lavender Dream kültivarı (%66,0) ve Sommerabend kültivarı (%67,0) en yüksekkök oluşumu yeteneği sergilemişlerdir. Bir büyüme düzenleyicisi olan Humifield'in 0,5 mg/l α-NAA ile kombinasyon halinde kullanılması, incelenen gül kültvarlarında köklenme oranının %70,0-86,0'a kadar artmasına katkıda bulunmuştur.

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Details

Primary Language	English
Subjects	Plant Biotechnology
Journal Section	Research Article/Araştırma Makalesi
Authors	Larysa Koldar This is me 0000-0002-6756-4172 Iryna Denysko This is me 0000-0002-2385-9331 Alla Konopelko 0000-0002-5214-6170 Yevhen Mazur This is me 0009-0009-8045-0768
Early Pub Date	October 11, 2024
Publication Date	October 15, 2024
Submission Date	April 5, 2024
Acceptance Date	October 2, 2024
Published in Issue	Year 2024 Volume: 25 Issue: 2

Cite

APA	Koldar, L., Denysko, I., Konopelko, A., Mazur, Y. (2024). Rhizogenesis in Shrub rose cultivated in vitro. Trakya University Journal of Natural Sciences, 25(2), 187-196. https://doi.org/10.23902/trkjnat.1464147
AMA	Koldar L, Denysko I, Konopelko A, Mazur Y. Rhizogenesis in Shrub rose cultivated in vitro. Trakya Univ J Nat Sci. October 2024;25(2):187-196. doi:10.23902/trkjnat.1464147
Chicago	Koldar, Larysa, Iryna Denysko, Alla Konopelko, and Yevhen Mazur. “Rhizogenesis in Shrub Rose Cultivated in Vitro”. Trakya University Journal of Natural Sciences 25, no. 2 (October 2024): 187-96. https://doi.org/10.23902/trkjnat.1464147.
EndNote	Koldar L, Denysko I, Konopelko A, Mazur Y (October 1, 2024) Rhizogenesis in Shrub rose cultivated in vitro. Trakya University Journal of Natural Sciences 25 2 187–196.
IEEE	L. Koldar, I. Denysko, A. Konopelko, and Y. Mazur, “Rhizogenesis in Shrub rose cultivated in vitro”, Trakya Univ J Nat Sci, vol. 25, no. 2, pp. 187–196, 2024, doi: 10.23902/trkjnat.1464147.
ISNAD	Koldar, Larysa et al. “Rhizogenesis in Shrub Rose Cultivated in Vitro”. Trakya University Journal of Natural Sciences 25/2 (October 2024), 187-196. https://doi.org/10.23902/trkjnat.1464147.
JAMA	Koldar L, Denysko I, Konopelko A, Mazur Y. Rhizogenesis in Shrub rose cultivated in vitro. Trakya Univ J Nat Sci. 2024;25:187–196.
MLA	Koldar, Larysa et al. “Rhizogenesis in Shrub Rose Cultivated in Vitro”. Trakya University Journal of Natural Sciences, vol. 25, no. 2, 2024, pp. 187-96, doi:10.23902/trkjnat.1464147.
Vancouver	Koldar L, Denysko I, Konopelko A, Mazur Y. Rhizogenesis in Shrub rose cultivated in vitro. Trakya Univ J Nat Sci. 2024;25(2):187-96.

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