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Rhizogenesis in Shrub rose cultivated in vitro

Year 2024, Volume: 25 Issue: 2, 187 - 196, 15.10.2024
https://doi.org/10.23902/trkjnat.1464147

Abstract

The study of the reproduction characteristics of roses of the garden class Shrub, the defini-tion of the dependence of the hormonal determination of explant rhizogenesis on the con-centrations of phytohormones that are part of the nutrient medium, are relevant and has both scientific and practical interest. This study presents the results of studies of hormonal de-termination of rhizogenesis in explants of cultivars of roses of the garden class Shrub: Gärt-nerfreude, Lavender Dream, Pomponella, Red Cascade, Sommerabend cultivated in vitro on nutrient medium containing growth regulators. It has been established that of the nutrient medium modified by the addition of 0.2-1.0 mg/l α-naphthylacetic acid (α-NAA), the most effective was the medium with the content of α-NAA 0.5 mg/l, the content of macro- and microelements half of the Murashige and Skoog prescriptions, and a decrease in the sucrose content to 2.0%. On this medium, the frequency of rhizogenesis averaged 61.2% for the studied cultivars. Hormonal determination of rhizogenesis and efficiency of root formation in vitro in the Shrub rose regenerants depended on the genotype of the plant: cv. Lavender Dream (66.0%) and cv. Sommerabend (67.0%) had the highest rhizogenesis ability. The use of the universal growth regulator Humifield in combination with 0.5 mg/l α-NAA contribut-ed to an increase in the rooting rate of the studied rose cultivars up to 70.0-86.0%.

References

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Year 2024, Volume: 25 Issue: 2, 187 - 196, 15.10.2024
https://doi.org/10.23902/trkjnat.1464147

Abstract

Çalı güllerinin üreme özelliklerinin incelenmesi ve eksplant kök oluşumunun hormonal kontrolünün kullanılan besleyici ortamın fitohormon konsantrasyonuna bağlı olduğunun tanımlanması önemlidir ve hem bilimsel hem de pratik açıdan üzerinde durulan konulardır. Bu çalışmada, büyüme düzenleyicileri içeren besin ortamı üzerinde in vitro olarak yetiştiri-len çalı gülü kültivarlarının (Gärtnerfreude, Lavender Dream, Pomponella, Red Cascade, Sommerabend) eksplantlarında kök oluşumunun hormonal belirlenmesine yönelik deneme-lerin sonuçları sunulmuştur. 0,2-1,0 mg/l a-naftilasetik asit (a-NAA) ilavesiyle modifiye edilen besin ortamlarından en etkilisinin, 0,5 mg/l a-NAA içerikli, sukroz oranı %2’ye kadar düşürülmüş ortam olduğu tespit edilmiştir. Bu ortamda, çalışılan kültivarlar için kök oluşu-mu sıklığı ortalama %61,2 olarak belirlenmiştir. Kullanılan kültivarlardaki kök oluşumunun in vitro etkinliğindeki hormonal katkının kültivarların genotiplerine bağlı olduğu belirlen-miştir: Lavender Dream kültivarı (%66,0) ve Sommerabend kültivarı (%67,0) en yüksekkök oluşumu yeteneği sergilemişlerdir. Bir büyüme düzenleyicisi olan Humifield'in 0,5 mg/l α-NAA ile kombinasyon halinde kullanılması, incelenen gül kültvarlarında köklenme oranının %70,0-86,0'a kadar artmasına katkıda bulunmuştur.

References

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There are 65 citations in total.

Details

Primary Language English
Subjects Plant Biotechnology
Journal Section Research Article/Araştırma Makalesi
Authors

Larysa Koldar This is me 0000-0002-6756-4172

Iryna Denysko This is me 0000-0002-2385-9331

Alla Konopelko 0000-0002-5214-6170

Yevhen Mazur This is me 0009-0009-8045-0768

Early Pub Date October 11, 2024
Publication Date October 15, 2024
Submission Date April 5, 2024
Acceptance Date October 2, 2024
Published in Issue Year 2024 Volume: 25 Issue: 2

Cite

APA Koldar, L., Denysko, I., Konopelko, A., Mazur, Y. (2024). Rhizogenesis in Shrub rose cultivated in vitro. Trakya University Journal of Natural Sciences, 25(2), 187-196. https://doi.org/10.23902/trkjnat.1464147
AMA Koldar L, Denysko I, Konopelko A, Mazur Y. Rhizogenesis in Shrub rose cultivated in vitro. Trakya Univ J Nat Sci. October 2024;25(2):187-196. doi:10.23902/trkjnat.1464147
Chicago Koldar, Larysa, Iryna Denysko, Alla Konopelko, and Yevhen Mazur. “Rhizogenesis in Shrub Rose Cultivated in Vitro”. Trakya University Journal of Natural Sciences 25, no. 2 (October 2024): 187-96. https://doi.org/10.23902/trkjnat.1464147.
EndNote Koldar L, Denysko I, Konopelko A, Mazur Y (October 1, 2024) Rhizogenesis in Shrub rose cultivated in vitro. Trakya University Journal of Natural Sciences 25 2 187–196.
IEEE L. Koldar, I. Denysko, A. Konopelko, and Y. Mazur, “Rhizogenesis in Shrub rose cultivated in vitro”, Trakya Univ J Nat Sci, vol. 25, no. 2, pp. 187–196, 2024, doi: 10.23902/trkjnat.1464147.
ISNAD Koldar, Larysa et al. “Rhizogenesis in Shrub Rose Cultivated in Vitro”. Trakya University Journal of Natural Sciences 25/2 (October 2024), 187-196. https://doi.org/10.23902/trkjnat.1464147.
JAMA Koldar L, Denysko I, Konopelko A, Mazur Y. Rhizogenesis in Shrub rose cultivated in vitro. Trakya Univ J Nat Sci. 2024;25:187–196.
MLA Koldar, Larysa et al. “Rhizogenesis in Shrub Rose Cultivated in Vitro”. Trakya University Journal of Natural Sciences, vol. 25, no. 2, 2024, pp. 187-96, doi:10.23902/trkjnat.1464147.
Vancouver Koldar L, Denysko I, Konopelko A, Mazur Y. Rhizogenesis in Shrub rose cultivated in vitro. Trakya Univ J Nat Sci. 2024;25(2):187-96.

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