Morphometric Effects of Testosterone Supplementation on Certain Extremity Bones in Young Swim-Trained Rats

Year 2018, , 140 - 144, 30.12.2018

Abdullah Kılcı Sefa Lok

https://doi.org/10.15314/tsed.466885

Cited By: 2

Abstract

There has been a dramatic
increase in the use of doping agents today. Used by athletes to increase
strength, endurance and speed, AAS lead to various negative effects on the human
body despite enhancing performance and strength. The purpose of this study was
to investigate the morphometric effects of testosterone supplementation on
certain extremity bones in young swim-trained rats. The study was conducted
with a total of 24 30-day-old male Wistar rats obtained from Selcuk University
Experimental Medicine Research and Application Center. The rats were divided into
four equal groups of six: control (C), exercise (E), testosterone (T) and
testosterone+exercise (TE) groups. The appropriate weekly dose was adjusted for
the rats in the testosterone-treated group according to their body weights. The
front and back extremity bones of the materials were dissected and the
uncovered humerus and femur bones were dried. The length, corpus thickness, cortex
cortical thickness and medulla diameter points of each bone were determined and
the morphometric measurements were taken. The results were presented as
Mean±SD. Data were analyzed through comparison between the groups by using
ANOVA and Duncan test. The significance level was set at P<0.05. The femur
and humerus lengths of the TE, T, E, and C groups were compared and the respective
lengths were femur; 32.24±1.04 for the C group, 32.23±0.28 for the E group,
31.12±0.73 for the TE group and 30.93±0.72 for the T group, humerus; 25,74±0,77
for the C group, 25,66±0,25 fot the E group, 24,68±0,53 for the TE group and
24,58±0,41 for the T group. The femur and humerus bones of the rats in the
groups given testosterone supplementation (TE and T) were significantly shorter
than those of the rats in the other two groups (p<0.05). However, there were
not any statistical differences among the TE, T, E, and C groups in terms of
cortex, corpus and medullary diameter measurements of the femur and humerus
bones (p>0.05).  The results of the study showed that
testosterone supplementation stopped the growth of femur and humerus by causing
premature epiphyseal closure in them. Also, even exercise did not reduce the
adverse effects of testosterone supplementation. Although some athletes think
that prohibited agents used as AAS affect performance positively, these agents
should not be used because of their adverse effects on athletes’ health and
because they are against sports ethics.

Keywords

Testosterone, Exercise, Femur, Humerus

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