Ultrastructure of aedeagus and spermatheca of Chaetocnema major (Jacquelin du Val) (Chrysomelidae: Galerucinae: Alticini) by scanning electron microscope

Year 2023, Volume: 4 Issue: 2, 116 - 132, 14.11.2023

Havva Ekmekci , Huseyin Özdikmen , Neslihan Bal , Damla Amutkan Mutlu , Zekiye Suludere

Abstract

The paper presents unknown ultrastructure of aedeagus and spermatheca of Chaetocnema major (Jacquelin du Val) which is the type species of the subgenus Chaetocnema (Majoroides) Özdikmen and the single species of the subgenus occurring in Türkiye, are studied and figured under both stereo microscope and Scanning Electron Microscope. Aedeagi and spermathecae have so far only been studied in the genus Chaetocnema Stephens based on stereo microscopic studies. Since no ultrastructural studies with SEM have been conducted on male and female genitalia of Chaetocnema species so far, this is the first study for this purpose. Accordingly, this study mainly aims to reveal and obtain new diagnostic characters by ultrastructural and detailed examinations of the aedeagus and spermathecae of Chaetocnema major. As is known, spermathecae of the genus Chaetocnema, like most genera of the tribe Alticini, are partially homogeneous and do not provide good diagnostic characters at the species level. Studies on the aedeagus have revealed that, despite the partial homogeneity of the spermatheca, the structure of the aedeagus shows quite a wide diversity. On the other hand, for the genus Chaetocnema, sperm sheath morphology at the subspecies level appears to be more relevant and useful than external morphological features. While the results from the present study mainly support these views, the female genitalia (spermathecae) was found to be insignificant and non-diagnostic, but important for diagnosis at the aedeagus species level, and further, investigation revealed new diagnostic characters for both Ultrastructural morphologies aedeagus and spermathecae by examining in SEM.

Keywords

Aedeagus, Chaetocnema major, spermatheca, SEM, Türkiye, Ultrastructure.

Project Number

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Chaetocnema major (Jacquelin du Val) (Chrysomelidae: Galerucinae: Alticini)’ün aedeagus ve spermateka'sının taramalı elektron mikroskobu ile ince yapısı

Abstract

Bu makale, Chaetocnema major (Jacquelin du Val) türünün aedeagus ve spermatekasının bilinmeyen ultrastrüktürünü sunmaktadır. Chaetocnema (Majoroides) Özdikmen alt cinsinin tip türleri ve Türkiye'de bulunan tek türü hem stereo mikroskop hem de Taramalı Elektron Mikroskobu altında incelenmiş ve figüre edilmiştir. Aedeagi ve spermathecae şimdiye kadar sadece Chaetocnema Stephens cinsinde stereo mikroskobik çalışmalara dayanarak incelenmiştir. Chaetocnema türlerinin erkek ve dişi genital organları üzerinde bugüne kadar SEM ile ultrastrüktürel bir çalışma yapılmadığından, bu çalışma bu amaca yönelik ilk çalışmadır. Buna göre, bu çalışma Chaetocnema major'un aedeagus ve spermateka'sının ultrastrüktürel ve detaylı incelemeleri ile yeni diagnostik karakterleri ortaya çıkarmayı ve elde etmeyi amaçlamaktadır. Bilindiği gibi, Chaetocnema cinsinin spermatekaları, Alticini kabilesinin çoğu cinsi gibi, kısmen homojendir ve tür düzeyinde iyi teşhis karakterleri sağlamaz. Aedeagus üzerine yapılan çalışmalar, spermatekanın kısmi homojenliğine rağmen, aedeagusun yapısının oldukça geniş bir çeşitlilik gösterdiğini ortaya koymuştur. Öte yandan, Chaetocnema cinsi için, alt tür düzeyinde sperm kılıfı morfolojisi, dış morfolojik özelliklerden daha ilgili ve yararlı görünmektedir. Mevcut çalışmadan elde edilen sonuçlar temel olarak bu görüşleri desteklerken, dişi genital organın (spermateka) önemsiz ve tanısal olmadığı, ancak aedeagus tür düzeyinde tanı için önemli olduğu bulunmuştur ve ayrıca, SEM'de incelenerek hem Ultrastrüktürel morfolojiler aedeagus hem de spermathecae için yeni tanısal karakterler ortaya çıkarılmıştır.

Keywords

Anahtar Kelimeler: Aedeagus, Chaetocnema major, spermatheca, SEM, Türkiye, Ultrastructure.

Ethical Statement

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Supporting Institution

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Project Number

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Thanks

This study is derived from the first author's doctoral thesis.

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