GENOTOXIC AND ANTIGENOTOXIC EFFECTS OF CYNARIN AGAINST MITOMYCIN-C INDUCED SISTER CHROMATID EXCHANGES
Abstract
Artichoke is a
plant that is cultivated for its head and leaves which can be eaten as a
vegetable. Numerous studies on artichokes have showed that it has
health-protective effect such as hepatoprotective, antioxidant,
hypocholesterolemic and anticarcinogenic activities. Cynarin is a polyphenol
that is derivative of di-caffeoylquinic acids in artichoke. It has strong
antioxidant activity. The present study was planned for the assessment of
potential in vitro genotoxic and antigenotoxic effect of cynarin against
mitomycin-C (MMC) by using sister chromatid exchange (SCE) assay in human
lymphocytes. Peripheral lymphocytes were incubated with different
concentrations of cynarin (6.25, 12.50, 25.00, 50.00, 100.00 µg/mL) alone and
simultaneously with 0.2 µg/mL MMC for 24 and 48 hours. A negative, a solvent
(50% methanol) and a positive control (MMC) were also maintained. Cynarin did
not significantly increased the SCE/cell frequency at all the concentrations
alone compared to control groups at both 24 h and 48 h (except 100 µg/mL).
Simultaneous treatment of Cynarin and MMC significantly reduced the frequency
of SCEs/cell in the three concentrations (12.50, 25.00 and 50.00 µg/mL)
compared to positive control in both application times. Our results suggested that
cynarin may have antigenotoxic potential especially at highest concentrations.
References
- Esra ERIKEL, Deniz YÜZBAŞIOĞLU, Fatma ÜNAL
- Gazi University, Science Faculty, Department of Biology, Genetic Toxicology Laboratory, 06500, Ankara, TURKEY
Abstract
References
- Esra ERIKEL, Deniz YÜZBAŞIOĞLU, Fatma ÜNAL
- Gazi University, Science Faculty, Department of Biology, Genetic Toxicology Laboratory, 06500, Ankara, TURKEY
Details
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|---|---|
| Publication Date | February 16, 2017 |
| IZ | https://izlik.org/JA33KL74EJ |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |