GENOTOXIC AND ANTIGENOTOXIC EFFECTS OF CYNARIN AGAINST MITOMYCIN-C INDUCED SISTER CHROMATID EXCHANGES

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 173 - 173, 16.02.2017

Esra Erıkel Deniz Yüzbaşıoğlu Fatma Ünal

Öz

Artichoke is a
plant that is cultivated for its head and leaves which can be eaten as a
vegetable. Numerous studies on artichokes have showed that it has
health-protective effect such as hepatoprotective, antioxidant,
hypocholesterolemic and anticarcinogenic activities. Cynarin is a polyphenol
that is derivative of di-caffeoylquinic acids in artichoke. It has strong
antioxidant activity. The present study was planned for the assessment of
potential in vitro genotoxic and antigenotoxic effect of cynarin against
mitomycin-C (MMC) by using sister chromatid exchange (SCE) assay in human
lymphocytes. Peripheral lymphocytes were incubated with different
concentrations of cynarin (6.25, 12.50, 25.00, 50.00, 100.00 µg/mL) alone and
simultaneously with 0.2 µg/mL MMC for 24 and 48 hours. A negative, a solvent
(50% methanol) and a positive control (MMC) were also maintained. Cynarin did
not significantly increased the SCE/cell frequency at all the concentrations
alone compared to control groups at both 24 h and 48 h (except 100 µg/mL).
Simultaneous treatment of Cynarin and MMC significantly reduced the frequency
of SCEs/cell in the three concentrations (12.50, 25.00 and 50.00 µg/mL)
compared to positive control in both application times. Our results suggested that
cynarin may have antigenotoxic potential especially at highest concentrations. 

Anahtar Kelimeler

GENOTOXIC AND ANTIGENOTOXIC EFFECTS OF CYNARIN AGAINST MITOMYCIN-C INDUCED SISTER CHROMATID EXCHANGES

Kaynakça

• Esra ERIKEL, Deniz YÜZBAŞIOĞLU, Fatma ÜNAL
• Gazi University, Science Faculty, Department of Biology, Genetic Toxicology Laboratory, 06500, Ankara, TURKEY