Abstract
Investigation
the histopathological effects of thiourea dioxide on intestine tissue of
zebrafish were aimed.
Thioureadioxide
is an organosulfur compound which is a strong reducing agent. It is generally
used as decolorisation agent, bleach in textile industry. In the other
industrial fields such as leather processing, photographic, paper, pulp and
board industry it has a wide range of use. Industrial waste products have been
contaminated aquatic environment day after day.
After one-week
adaptation period zebrafish divided into three group (n=10) as one control and
2 experimental groups (3mM, 4.5mM). For investigating the effects of thiourea
dioxide, intestine tissues were dissected after 5 day of the exposure. Tissues
were fixed with 10% neutral buffered formalin and dehydration were carried out
in an ascending series of ethanol. Tissues were cleared in xylene, embedded in
paraffin wax and cut into 5 µm sections on a microtome. The sections were
stained with hematoxylin (H&E). Results were evaluated with light
microscope.
In control group
normal intestine histology have been observed. In 3 mM thiourea dioxide exposed
group, hypertrophy and edema at muscularis externa, degeneration and necrosis
at villi structure, deterioration and vacuolization at lamina propria and
increase in the number of goblet cells were observed. In 4.5 mM thiourea
dioxide exposed group, severe edema and hemorrhage in muscularis externa,
degeneration at villi structure and vacuolization at mucosa layer were
monitored.
Keywords
HISTOPATHOLOGICAL EFFECTS OF THIOUREA DIOXIDE ON INTESTINE TISSUE OF ZEBRAFISH (Danio rerio
References
- Cansu AKBULUT, Büşra FİDAN, Mustafa ZENGİN, Nazan Deniz YÖN
- Sakarya University Science and Letters Faculty Department of Biology, Sakarya, Turkey Sakarya University Science and Letters Faculty Department of Chemistry, Sakarya, Turkey
Abstract
References
- Cansu AKBULUT, Büşra FİDAN, Mustafa ZENGİN, Nazan Deniz YÖN
- Sakarya University Science and Letters Faculty Department of Biology, Sakarya, Turkey Sakarya University Science and Letters Faculty Department of Chemistry, Sakarya, Turkey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
