Abstract
Objectives
Cefazolin, a first generation
cephalosporin β-lactam antibiotic, is commonly used for
prophylaxis during cesarean delivery, and in treatment of pulmonary infections
and many surgical procedures. The aim of this work was to develop a simple,
fast and accurate liquid-chromatography-mass spectrometry method for
determination of serum cefazolin.
Methods
Mass spectrometric analyses were
performed using an Shimadzu LC-20-AD (Kyoto, Japan) coupled with a ABSCIEX API
3200 triple quadrupole mass spectrometer (USA) equipped with an electrospray
ion source (ESI) operating in positive mode.
Results
The standard curves for cefazolin was
linear within the range of 0.39-200 µg/ml. Total run time was 5 minutes.
Chromatographic separation was performed on a C18 column
mm, 5 µm, Phenomenex Luna)
formic acid in water and MeOH
at a flow rate of 0.8 ml/min.
Discussion
Serum cefazolin measurement can be
easily performed by LC-MS/MS system to identify the risk of the patients with
therapeutic drug monitoring. This method performs this drug analysis with high
throughput.
Conclusions
Exact determination of cefazolin
provides reliable levels in clinical studies to avoid toxic effects.
Keywords
Antibiotic levels, mass spectrometry,
cefazolin, toxicity.
Acknowledgments: No conflict of
interest
References
- Ali Unlu*, Sedat Abusoglu, Abdullah Sivrikaya
- Department of Biochemistry, SelcukUniversity Faculty of Medicine, Konya, Turkey
Abstract
References
- Ali Unlu*, Sedat Abusoglu, Abdullah Sivrikaya
- Department of Biochemistry, SelcukUniversity Faculty of Medicine, Konya, Turkey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | November 1, 2017 |
| Published in Issue | Year 2017 Volume 2, Issue 1(4) |