Mass Spectrometric Quantitation of Serum Cephazolin

Yıl 2017, Volume 2, Issue 1(4), 61 - 61, 01.11.2017

Ali Unlu Sedat Abusoglu Abdullah Sivrikaya

Öz

Objectives

Cefazolin, a first generation
cephalosporin β-lactam antibiotic, is commonly used for
prophylaxis during cesarean delivery, and in treatment of pulmonary infections
and many surgical procedures. The aim of this work was to develop a simple,
fast and accurate liquid-chromatography-mass spectrometry method for
determination of serum cefazolin.

 

Methods

Mass spectrometric analyses were
performed using an Shimadzu LC-20-AD (Kyoto, Japan) coupled with a ABSCIEX API
3200 triple quadrupole mass spectrometer (USA) equipped with an electrospray
ion source (ESI) operating in positive mode.

Results

The standard curves for cefazolin was
linear within the range of 0.39-200 µg/ml. Total run time was 5 minutes.
Chromatographic separation was performed on a C18 column (4.6×50
mm, 5 µm, Phenomenex Luna) with a mobile phase consisting of 1%
formic acid in water and MeOH (10:90, V/V)
at a flow rate of 0.8 ml/min.

 

Discussion

Serum cefazolin measurement can be
easily performed by LC-MS/MS system to identify the risk of the patients with
therapeutic drug monitoring. This method performs this drug analysis with high
throughput.

 

Conclusions

Exact determination of cefazolin
provides reliable levels in clinical studies to avoid toxic effects.

 

Keywords

Antibiotic levels, mass spectrometry,
cefazolin, toxicity.

 

Acknowledgments: No conflict of
interest

Anahtar Kelimeler

Mass Spectrometric Quantitation of Serum Cephazolin

Kaynakça

• Ali Unlu*, Sedat Abusoglu, Abdullah Sivrikaya
• Department of Biochemistry, SelcukUniversity Faculty of Medicine, Konya, Turkey