Comparison of different extraction methods for total phenolic content and antioxidant activity of dried Diospyros lotus L fruits

Abstract

The yield, total phenolic content and antioxidant activity values of the extracts obtained from the dried fruits of Diospyros lotus L. plant by using Soxhlet (SXE), ultrasound assisted extraction (UBE) and hot solvent extraction (HME) techniques with methanol were investigated. The highest extraction yield was obtained from HME experiments with 50.67±0.63% and UBE with 49.50±1.05%, respectively. While the extract obtained by the UBE technique showed lower TPC value (1464±57 mg GAE/100 g original sample) compared to the extracts obtained from the other two techniques, it showed higher antioxidant activity values than that of HME technique. While these values were determined as 192.53±4.45 and 273.10±34.79 mg/mL (IC50, lower is better) for the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay, 0.65±0.04 and 0.25±0.04 g GAE/100 g original sample for cupric ion reducing antioxidant activity (CUPRAC) assay, respectively. Both the UBE and HME techniques have clearly demonstrated that they are more advantageous than conventional Soxhlet extraction for simplicity of application, reduced solvent consumption, extraction of thermally sensitive compounds and shortened extraction times.

Keywords

• Diospyros lotus L
• Soxhlet Extracion
• Ulrasound-Assisted Extraction
• Hot Solvent Extraction
• Total Phenolic Content
• Antioxidant Activity

References

1. D. Thompson, P. Moldéus, Cytotoxicity of butylated hydroxyanisole and butylated hydroxytoluene in isolated rat hepatocytes, Biochemical Pharmacology, 37, 1988, 2201–7. https://doi.org/10.1016/0006-2952(88)90582-5
2. J.M. Luque-Rodriquez, M.D. Luque De Castro, P. Perez-Juan, Extraction of fatty acids from grape seed by superheated hexane, Talanta, 68 (1), 2005, 126-30. https://doi.org/10.1016/j.talanta.2005.04.054
3. R.P.F.F. da Silva, T.A.P. Rocha-Santosa, A.C. Duarte, Supercritical fluid extraction of bioactive compounds, Trends Anal. Chem, 76, 2016, 40-51. https://doi.org/10.1016/j.trac.2015.11.013
4. K. Kumar, S. Srivastav, V.S. Sharanagat, Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products: A review, Ultrason Sonochem, 70, 2020, 105325. https://doi.org/10.1016/j.ultsonch.2020.105325
5. A.P. Da Fonseca Machado, J.L. Pasquel-Reátegui, G.F. Barbero, J. Martínez, Pressurized liquid extraction of bioactive compounds from blackberry (Rubus fruticosus L.) residues: a comparison with conventional methods, Food Res Int, 77 (3), 2015, 675-83. https://doi.org/10.1016/j.foodres.2014.12.042
6. S. B. Bagade, M. Patil, Recent Advances in Microwave Assisted Extraction of Bioactive Compounds from Complex Herbal Samples: A Review, Crit Rev Anal Chem, 51 (2), 2021, 138-49. https://doi.org/10.1080/10408347.2019.1686966
7. T. Baytop, Türkiye’de Bitkiler ile Tedavi: Geçmişte ve Bugün, 1999, İstanbul: Nobel Tıp Kitabevleri, s. 138, (in Turkish)
8. M.R. Loizzo, A. Said, R. Tundis, U.W. Hawas, K. Rashed, F. Menichini, N.G. Frega, F. Menichini, Antioxidant and antiproliferative activity of Diospyros lotus L. extract and isolated compounds, Plant Foods Hum Nutr, 64 (4), 2009, 264-70. https://doi.org/10.1007/s11130-009-0133-0

9. S.M. Nabavi, M.A. Ebrahimzadeh, S.F. Nabavi, M. Fazelian, B. Eslami, In vitro Antioxidant and Free Radical Scavenging Activity of Diospyros lotus and Pyrus boissieriana growing in Iran, Phcog Mag, 5 (18), 2009, 122-6.
10. H. Gao, N. Cheng, J. Zhou, B. Wang, J. Deng, W. Cao, Antioxidant activities and phenolic compounds of date plum persimmon ( Diospyros lotus L.) fruits, J Food Sci Technol, 51 (5), 2014, 950-6. https://doi.org/10.1007/s13197-011-0591-x
11. F.A. Ayaz, A. Kadioglu, Fatty acid compositional changes in developing persimmon (Diospyros lotus L.) fruit, New Zealand J Crop Hort Sci, 1999, 27 (3), 257-61. https://doi.org/10.1080/01140671.1999.9514104
12. K. Slinkard, V.L. Singleton, Total Phenol Analysis: Automation and Comparison with Manual Methods, Am J Enol Viticult, 1977, 28, 49–55.
13. M. Cuendet, K. Hostettmann, O. Potterat, W. Dyatmiko, Iridoid Glucosides with Free Radical Scavenging Properties from Fagraea blumei, Helv Chim Acta, 1997, 80 (4), 1144-52. https://doi.org/10.1002/hlca.19970800411
14. R. Apak, K. Güçlü, M. Özyürek, S.E. Karademir, Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method, J Agric Food Chem, 2004, 52, 7970-81. https://doi.org/10.1021/jf048741x
15. F. Akdeniz, M. Gündoğdu, Direct and alkali medium liquefaction of Laurocerasus officinalis Roem, Energy Convers Manage, 2007, 48, 189-92. https://doi.org/10.1016/j.enconman.2006.04.018
16. D.D. Gertenbach, Solid–liquid extraction technologies for manufacturing nutraceuticals from botanicals, In: Functional foods: Biochemical and processing aspects, J. Shi, G. Mazza, M. Le Maguer (Eds.), 2001, Boca Ratón, FL, CRC Press Inc.
17. S.S. Petrović, J. Ivanović, S. Milovanović, I. Žižović, Comparative analyses of the diffusion coefficient from thyme for different extraction processes, J. Serb. Chem. Soc, 2012, 77 (6), 799–813. https://doi.org/10.2298/JSC110616009P
18. A. Dobrinčić, M. Repajić , I.E. Garofulić, L. Tuđen, V. Dragović-Uzelac and B. Levaj, Comparison of different extraction methods for the recovery of olive leaves polyphenols, Processes, 2020, 8 (9), 1008. https://doi.org/10.3390/pr8091008
19. J.O. Chaves, M.C. de Souza, L.C. da Silva, D. Lachos-Perez, P.C. Torres-Mayanga, A.P.F. Machado, T. Forster-Carneiro, M. Vázquez-Espinosa, A.V. González-de-Peredo, G.F. Barbero, M.A. Rostagno, Extraction of Flavonoids From Natural Sources Using Modern Techniques, Front. Chem., 2020, 8, 864. https://doi.org/10.3389/fchem.2020.507887
20. N. Medina-Torres, T. Ayora-Talavera, H. Espinosa-Andrews, A. Sánchez-Contreras, N. Pacheco, Ultrasound assisted extraction for the recovery of phenolic compounds from vegetable sources, Agronomy, 2017, 7(3), 47. https://doi.org/10.3390/agronomy7030047
21. L.K. MacDonald-Wicks, L.G. Wood, M.L. Garg, Methodology for the determination of biological antioxidant capacity in vitro: a review, J. Sci. Food. Agric, 2006, 86 (13), 2046. https://doi.org/10.1002/jsfa.2603
22. M.M. Küçük, S. Ağırtaş, Liquefaction of Prangmites australis by supercritical gas extraction, Bioresour. Technol., 1999, 69 (2), 141-143. https://doi.org/10.1016/S0960-8524(98)00180-1