Comparison of different extraction methods for total phenolic content and antioxidant activity of dried Diospyros lotus L fruits

Öz

The yield, total phenolic content and antioxidant activity values of the extracts obtained from the dried fruits of Diospyros lotus L. plant by using Soxhlet (SXE), ultrasound assisted extraction (UBE) and hot solvent extraction (HME) techniques with methanol were investigated. The highest extraction yield was obtained from HME experiments with 50.67±0.63% and UBE with 49.50±1.05%, respectively. While the extract obtained by the UBE technique showed lower TPC value (1464±57 mg GAE/100 g original sample) compared to the extracts obtained from the other two techniques, it showed higher antioxidant activity values than that of HME technique. While these values were determined as 192.53±4.45 and 273.10±34.79 mg/mL (IC50, lower is better) for the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay, 0.65±0.04 and 0.25±0.04 g GAE/100 g original sample for cupric ion reducing antioxidant activity (CUPRAC) assay, respectively. Both the UBE and HME techniques have clearly demonstrated that they are more advantageous than conventional Soxhlet extraction for simplicity of application, reduced solvent consumption, extraction of thermally sensitive compounds and shortened extraction times.

Anahtar Kelimeler

• Diospyros lotus L
• Soxhlet Extracion
• Ulrasound-Assisted Extraction
• Hot Solvent Extraction
• Total Phenolic Content
• Antioxidant Activity

Kaynakça

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