Farklı sitokinin ve oksin kaynaklarının Kalanchoe blossfeldiana mikroçoğaltımı üzerine etkisi

Year 2024, , 1182 - 1192, 12.10.2024

Rahma Bejaouı , Şeküre Şebnem Ellialtıoğlu , Ruhsar Yanmaz

https://doi.org/10.30910/turkjans.1531762

Abstract

Mikroçoğaltım, aseptik koşullar altında birçok farklı süs ve tıbbi bitki türünü vejetatif olarak çoğaltmak için yaygın olarak kullanılmaktadr. Bu teknoloji, mevsim ve zaman-mekan kısıtlamalarından bağımsız olarak yaygın pratik uygulamalara sahiptir. Bu çalışma, Kalanchoe blossfeldiana yaprak sapına yakın yaprak segmentleri eksplant olarak kullanılarak mikroçoğaltım için ticari çay şekeriyle desteklenmiş 18 adet farklı BAP, NAA, TDZ ve GA3 kombinasyonunu kullanarak in vitro çoğaltım ve yaşayabilir kültürler için verimliliğini bulmayı amaçlamamıştır. Sonuçlar, TDZ ve BAP destekli rejenerasyon kültürleri üzerinde rejenerasyon arasında net bir ayrım göstermiştir. Dolayısıyla, hem BAP - NAA hem de TDZ - NAA konbinasyonları mikroçoğaltım için uygun bulunmuştur. Sürgün rejenerasyon ortamından bağımsız olarak, sürgün rejenerasyon ortamının kaynak etkisi göstermiştir ve sürgünlerin köklenme yeteneğine yansımıştır. Bu bitkicikler 0,01-0,05 mg L-1 IBA'da köklendirilmiştir. Çalışmanın sonuçları, 0,5 mg L-1 IBA ile 1 ½ veya 1×MS ortamı kullanılarak in vitro koşullarda başarılı köklenme gösterilmiştir. Daha sonra bu köklendirilmiş bitkicikler, adaptasyon için 0,2 mg L-1 gibberellik asitle ve BAP'siz veya 0,1, 0,2, 0,5 mg L-1 TDZ'li ve 0,1 mg L-1 NAA'li veya 0,1 mg L-1 NAA'siz MS ortamına aktarılmıştır. Çalışmanın sonuçları, K. blossfeldiana bitki ıslah çalışmaları ve yetiştiricileri için büyük önem taşımaktadır.

Keywords

: PGR, ticari çoğaltım, in vitro, mikroçoğaltım, sürgün rejenerasyonu, Kalanchoe

The effect of different cytokinin and auxin source on micropropagation of Kalanchoe blossfeldiana

Abstract

Micropropagation is widely used for vegetative propagation of many different ornamental and medicinal plant species under aseptic conditions. This technology has widespread practical applications irrespective of seasonal and time-space constraints. The present study aimed to design in vitro propagation using 1 8 different combinations of BAP, NAA, TDZ, and GA3 supplemented with commercial tea sugar at 30 g l-1 for micropropagation using leaf segments close to petiole of Kalanchoe blossfeldiana as explants and to find its efficiency for viable cultures. The results showed clear discrimination between regeneration on TDZ and BAP-supplemented regeneration cultures. Thus, both BAP-NAA and TDZ-NAA combinations were found suitable for micropropagation. the source medium affected the shoot regeneration and the rooting ability, irrespective of the culture of any shoot regeneration medium. These plantlets were rooted in 0.01-0.05 mg l-1 IBA. The study showed successful rooting under in vitro conditions using 1 ½ or 1×MS medium with 0.5 mg L-1 IBA. These rooted plantlets were then transferred to MS medium with 0.2 mg L-1 gibberellic acid and without BAP or with 0.1, 0.2, 0.5 mg l-1 TDZ and 0.1 mg l-1 NAA or without 0.1 mg L-1 NAA for adaptation. The results of the study are of great importance for K. blossfeldiana plant breeding studies and growers.

Keywords

PGR, commercial multiplication, in vitro, micropropagation, shoot regeneration, Kalanchoe

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