A Study on the Presence of Potato Cyst Nematodes: Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 and Globodera pallida Stone 1973 (Tylenchida: Heteroderidae) in Nevşehir Province, Turkey

Year 2019, , 125 - 129, 22.04.2019

Adem Özarslandan , Dilek Dınçer Refik Bozbuga Mustafa İmren İbrahim Halil Elekcıoğlu

https://doi.org/10.30910/turkjans.556586

Cited By: 2

Abstract

Potato (Solanum tuberosum L.) cyst nematodes (Globodera rostochiensis (Wollenweber)
and Globodera pallida (Stone) cause
significant yield losses and have been listed as quarantine pests for many
countries in the world including Turkey, and the European and Mediterranean
Plant Protection Organization (EPPO, A2). In Turkey, the current potato
production area is decreasing due to contamination by quarantine agents, and
new fields are under threat of invasion by new quarantine organisms. In this
study, samples were collected from Nevşehir province, Turkey, in potato growing
fields during the spring growing season in 2014-2015. Soil samples were washed
using Fenwick’s can and cysts were extracted and identified using molecular
markers. The species-specific molecular markers generated a 435 bp using
PITSr3, PITSp4 and ITS5 primers. The potato golden cyst nematode, G. rostochiensis was identified in
Nevşehir potato production areas and this is the first report for the Central
Anatolia Region. In addition, G.
rostochiensis was found in two samples of the same field in the surveys. The
average cyst nematode density was determined as 60 cysts in 250 g soil.

Keywords

Potato, cyst nematode, molecular identification, density

Nevşehir İlinde Patates Kist Nematodlarının Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 ve Globodera pallida Stone 1973 (Tylenchida: Heteroderidae) Belirlenmesi

Abstract

Patates
(Solanum tuberosum L.) kist
nematodları (Globodera rostochiensis
(Wollenweber) ve Globodera pallida
(Stone) önemli verim kayıplarına neden olmakta, Türkiye de dahil olmak üzere
dünyadaki birçok ülke, Avrupa ve Akdeniz Bitki Koruma Örgütü’nde (EPPO, A2)
karantina zararlıları olarak listelenmiştir. Türkiye'de mevcut patates üretim
alanı karantina zararlısı olarak bulaşmaya bağlı olarak azalmakta ve yeni
alanlar yeni karantina organizmaları tarafından istila tehdidi altında
bulunmaktadır. Bu çalışmada, Türkiye, Nevşehir ilinden 2014-2015 yıllarında
bahar mevsiminde patates yetiştirme alanlarında örnekler toplanmıştır. Toprak örnekleri Fenwick aletinde yıkanarak
bulaşık örneklerdeki kistler elde edilmiş ve kist popülasyonlarının moleküler olarak teşhisleri yapılmıştır. Kist popülasyonlarının
moleküler düzeyde türe özgü PITSr3,
PITSp4 ve ITS5 primerleri kullanılarak yapılan teşhiste söz konusu primerlerin
435 bp de bant oluşturduğu tespit edilmiştir. Bu çalışma sonucunda Nevşehir ili patates ekiliş alanlarında patates kist
nematodu G. rostochiensis saptanmış
olup, bu sonuç İç Anadolu Bölgesi için ilk kayıt olma özelliğini taşımaktadır.
Ayrıca, sürveylerde aynı tarlaya ait iki örnekte G. rostochiensis’e rastlanılmış,
250 g toprakta ortalama 60 kist yoğunluğunda olduğu tespit edilmiştir.

Keywords

Patates, kist nematodu, moleküler teşhis, yoğunluk

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