Çorum Çevresindeki Yayla Topraklarından İzole Edilen Actinobacteria Üyelerinin Biyosentetik Genlerinin Taranması

Year 2021, , 280 - 285, 23.04.2021

Demet Tatar , Aysel Veyisoğlu

https://doi.org/10.30910/turkjans.715700

Abstract

Bu çalışmada, Çorum (Kargı ve Osmancık) çevresindeki yaylalardan izole edilen aktinomiset izolatlarının poliketit sentaz ve ribozomal olmayan peptit sentetaz biyosentetik sistemlerinin varlığı moleküler tekniklerle araştırılmıştır. Toplamda 15 tane Actinobacteria üyesinin sekonder metabolit biyosentetik genlerinin (PKS I-II/NRPS) varlığı PCR temelli incelenmiştir. Yapılan çalışmanın sonuçlarına göre, 15 tane aktinomiset izolatının 7 tanesi PKS-I, 11 tanesi PKS-II ve 14 tanesinin NRPS gen bölgesine sahip olduğu bulunmuştur. Sonuçlar değerlendirildiğinde izolatların nonribozomal peptid sentetaz (NRPS) gen kümelerini tip I-II poliketit sentazlar (PKS-I-II) gen kümelerinden daha fazla içerdiği görülmüştür. Ayrıca 5 izolatın üç gen kümesini bir arada bulundurduğu tespit edilmiştir. Sonuç olarak yaylalardan izole edilen aktinomisetlerin yüksek sekonder metabolit üretme kapasitesine sahip olduğu görülmüştür.

Keywords

Actinobacteria, Yayla Toprağı, PKS-I, PKS-II, NRPS, Biyosentetik Genler

Supporting Institution

Hitit Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir.

Project Number

ODMYO19003.18.001

Thanks

Çalışmaya olan katkılarından dolayı Ondokuz Mayıs Üniversitesi Moleküler Biyoloji ve Genetik Bölüm Başkanı Prof. Dr. Nevzat ŞAHİN’e şükranlarımı sunuyorum.

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