Immunological Diagnostic of Phytophthora infestans from Host Tissues (Potato) by ELISA Method

Year 2017, Volume: 4 Issue: 4, 385 - 392, 26.10.2017

Touseef Hussaın Bir Pal Sıngh Firoz Anwar

Abstract

Oomycete
pathogens of the genus Phytophthora are the most destructive plant
pathogens known. They spread mainly through the movement of infested
soil, water and infected plants and plant material. Especially damaging as a
source of inoculum are those plants/seeds that are infected but do not show
signs of symptoms either because the disease has not yet progressed to the
stage where symptoms are evident, or due to suppression of symptom development
by the use of fungicides. In our indirect ELISA method, P.infestans
exhibited strong positive reaction with sporangia (2.256), mycelium (1.256) as
well as oospores (2.286) whereas no reaction with other fungal pathogens of
potato. P.infestans was detected by indirect ELISA in potato leaves
(1.212) and tubers (1.201). Our study was to confirm the detection of P.infestans
irrespective of inoculum present in the host tissues (0.435), by which planting
material by quarantine dept., horticultural dept. from one state to another. It
could be used as reliable routine diagnostic test to replace current
identification and isolation, detection methods.

Keywords

Phytophthora infestans, oospores, potato, ELISA

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