Studying Structural Differentiation of Plant Parts of Sideritis pisidica Boiss. & Heldr. Using FTIR Spectroscopy

Year 2017, Volume: 4 Issue: 4, 461 - 467, 26.10.2017

Muharrem Ergun , Zahide Süslüoğlu Aydın Şükrü Bengü

Abstract

Fourier
Transform Infrared Spectroscopy (FTIR) is a novel tool for exploring
compositional structures of diverse organic or inorganic compounds employed by
many researches from physicists to horticulturists due to its time-saving and
easy-to-use application. FTIR was performed to differentiate structural
differentiation of plant parts of wild-grown Sideritis pisidica Boiss. & Heldr. from Turkey. Some minerals
Na, Cr, Zn, Fe, Mg, Mn, Co, Cu and Ni; protein, lipid, soluble sugar and
moisture contents in the stalk, leaf and blossom of the plant were analyzed
whether the FTIR spectra may predict major structural compositions. As expected
most of the minerals were presented higher amount in the leaf followed by the
blossom and stalk. The blossom was however found be richer in Ca, Cr, Ni and Zn
than leaf and stalk. Total protein contents of the leaf were higher than those
of the stalk or the leaf. Crude lipid amounts were however more prominent in
the stalk followed by the blossom and leaf respectively, unlike the total
protein contents. The blossom was found be richer in moisture content than the
leaf and stalk followed, respectively. The FTIR spectra of the plant parts
clearly revealed that protein and total soluble sugar contents are varied in
stalk, leaf or blossom of Sideritis
pisidica Boiss. & Heldr. interpreted from the peak intensities. The
spectra however did not reveal the crude lipid or moisture contents of the
plant parts.

Keywords

Herbal tea, Mountain tea, FTIR, Sideritis spp, Dedegöl Mountain

FTIR Spektroskopisi Kullanarak Sideritis pisidica Boiss. & Heldr. Bitki Aksamlarındaki Yapısal Farklılığın Araştırılması

Abstract

Fourier dönüşümlü
kızılötesi spektroskopisi (FTIR) kısa sürede ve kolay uygulanabilmesi nedeni
ile fizik bilim insanlarından bahçe bitkileri bilim insanlarına kadar birçok
araştırmacının başvurduğu değişik organik ve inorganik maddelerin yapılarını
araştırmak amacıyla kullanılan yenilikçi bir yöntemdir. Bu çalışmada, FTIR,
Türkiye’de doğal olarak yetişen yabani Sideritis
pisidica Boiss. & Heldr. bitki aksamlarındaki farklılığı ortaya koymak
için kullanılmıştır. Bitkinin gövdesi, yaprakları ve çiçeklerinde bulunan Na,
Cr, Zn, Fe, Mg, Mn, Co, Cu ve Ni gibi bazı mineraller ile protein, yağ, çözünür
şeker ve nem içeriği, FTIR spektrumlarının ana yapısal içerikleri tahmin edip
edemeyeceği amacıyla analiz edilmiştir. Tahmin edildiği gibi, çoğu minerallerin
miktarı yaprakta yüksek miktarda bulunmuş ve yaprağı sırası ile çiçek ve gövde
takip etmiştir. Bununla birlikte çiçek, yaprak ve gövdeye göre Ca, Cr ve Zn
mineralleri bakımından daha zengin bir içeriğe sahip olmuştur. Yaprak, gövde ve
çiçeğe göre daha fazla protein içermiştir. Toplam proteinin aksine, ham yağ
içeriği gövdede en yüksek bulunmuş ve gövdeyi sırası ile çiçek ve yaprak takip
etmiştir. Pik içeriği dikkate alındığında, FTIR spektrumları Sideritis pisidica Boiss. & Heldr.
bitkisinin gövde, yaprak ve çiçekte bulunan protein veya toplam çözünür şeker
içeriklerindeki farklığı açıkça ortaya koymuştur. Fakat spektrumlar bitki
aksamlarındaki ham yağ veya nem içeriğini tahmin edememiştir.

 

Keywords

Bitkisel çay, dağ çayı, FTIR, Sideritis spp, Dedegöl Dağı

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