Abstract
In vitro clonal propagation
and acclimatization of the tissue culture plant of large
cardamom has been established at Agriculture Research Station Pakhribas,
Dhankuta, Nepal. The rhizome buds were collected from the screen house and then
cultured in Murashige and Skoog (MS) medium enriched with 9 different
concentrations of BAP (6-benzylaminopurine) and IBA (indole-3-butyric acid)
namely 0.5 mg/L (BAP), 1.0 mg/L (BAP), 1.5 mg/L (BAP), 2.0 mg/L (BAP), 1 mg/L (IBA), 0.5 mg/L (BAP)+ 1 mg/L (IBA),
1.0 mg/L (BAP)+ 1 mg/L (IBA), 1.5 mg/L (BAP)+ 1 mg/L (IBA) and 2.0 mg/L (BAP)+
1 mg/L (IBA). This study showed that the explants grown in MS medium
supplemented with 1.0 mg / L BAP + 1.0 mg / L IBA showed the maximum root
induction rate. Buds produced roots in the same medium. The roots planted in
the screen house were transplanted for the hardening process. Then these
hardened plants were transferred to the netted nursery for further
multiplication process. This protocol developed could be used for developing
the superior quality plants of large cardamom.
References
- Balachandran, S.M., Baht, S.R., Chandel, K.P.S. 1990. In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.). Plant Cell Reports. 8: 521-524.
- Bhat, S.R., Chandel, K.P.S., Kacka, A. 1994. In vitro induction of rhizomes in ginger (Zingiber officinale Rosc.). Indian J. Exp. Biol. 32: 340-344.
- Hashim, H.M., Ibrahim, H., Rahim, Z. 1998. Preliminary studies on some nutritional composition of the edible gingers. In: Advance in Biochemistry and Biotechnology in Asia and Oceania. Proceedings of the 7th Federation of Asia and Oceania Biochemists Symposium (FAOB). El-2.
- Manohari, C., Backiyarani, S., Jebasingh, T., Somanath, A., Usha, R. 2008. Efficient plant regeneration in small cardamom (Elettaria cardamomum Maton.) through somatic embryogenesis. Indian Journal of Biotechnology. 7: 407-409.
- Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
- Pradhan, S., Pradhan, S., Basistha, B.C., Subba, K.B. 2014. In vitro micropropagation of Amomum subulatum (zingiberaceae), a major traditional cash crop of Sikkim Himalaya. Int. J. LifeSc. Bt& Phar. Res. 3(2): 169-180.
- Sajina, A., Mini, P.M., John, C.Z., Nirmalbabu, K., Ravindran, P.N., Peter, K.V. 1997. Micropropagation of large cardamom (Amomum subulatum Roxb.). Journal of Spices and Aromatic Crops 6(2): 145-148.
- Sharma, T.R., Singh, B.M. 1995. In vitro microrhizome production in Zingiber officinales Rosc. Plant cell Rep. 15: 274-277.
- Smith, M.K., Hamill, S.D. 1996. Field evaluation of micropropagated and conventionally propagated ginger in subtropical Queensland. Australian J. Exp. Agricul. 36: 347-354.
- Swar, S., Pant, B. 2004. Micropropagation of Cymbidium iridiodes D. Don. In: Proceeding 4th National Conference on Science and Technology, March 23-26, RONAST, Kathmandu, Nepal.
Abstract
In vitro clonal propagation
and acclimatization of the tissue culture plant of large
cardamom has been established at Agriculture Research Station Pakhribas,
Dhankuta, Nepal. The rhizome buds were collected from the screen house and then
cultured in Murashige and Skoog (MS) medium enriched with 9 different
concentrations of BAP (6-benzylaminopurine) and IBA (indole-3-butyric acid)
namely 0.5 mg/L (BAP), 1.0 mg/L (BAP), 1.5 mg/L (BAP), 2.0 mg/L (BAP), 1 mg/L (IBA), 0.5 mg/L (BAP)+ 1 mg/L (IBA),
1.0 mg/L (BAP)+ 1 mg/L (IBA), 1.5 mg/L (BAP)+ 1 mg/L (IBA) and 2.0 mg/L (BAP)+
1 mg/L (IBA). This study showed that the explants grown in MS medium
supplemented with 1.0 mg / L BAP + 1.0 mg / L IBA showed the maximum root
induction rate. Buds produced roots in the same medium. The roots planted in
the screen house were transplanted for the hardening process. Then these
hardened plants were transferred to the netted nursery for further
multiplication process. This protocol developed could be used for developing
the superior quality plants of large cardamom.
References
- Balachandran, S.M., Baht, S.R., Chandel, K.P.S. 1990. In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.). Plant Cell Reports. 8: 521-524.
- Bhat, S.R., Chandel, K.P.S., Kacka, A. 1994. In vitro induction of rhizomes in ginger (Zingiber officinale Rosc.). Indian J. Exp. Biol. 32: 340-344.
- Hashim, H.M., Ibrahim, H., Rahim, Z. 1998. Preliminary studies on some nutritional composition of the edible gingers. In: Advance in Biochemistry and Biotechnology in Asia and Oceania. Proceedings of the 7th Federation of Asia and Oceania Biochemists Symposium (FAOB). El-2.
- Manohari, C., Backiyarani, S., Jebasingh, T., Somanath, A., Usha, R. 2008. Efficient plant regeneration in small cardamom (Elettaria cardamomum Maton.) through somatic embryogenesis. Indian Journal of Biotechnology. 7: 407-409.
- Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
- Pradhan, S., Pradhan, S., Basistha, B.C., Subba, K.B. 2014. In vitro micropropagation of Amomum subulatum (zingiberaceae), a major traditional cash crop of Sikkim Himalaya. Int. J. LifeSc. Bt& Phar. Res. 3(2): 169-180.
- Sajina, A., Mini, P.M., John, C.Z., Nirmalbabu, K., Ravindran, P.N., Peter, K.V. 1997. Micropropagation of large cardamom (Amomum subulatum Roxb.). Journal of Spices and Aromatic Crops 6(2): 145-148.
- Sharma, T.R., Singh, B.M. 1995. In vitro microrhizome production in Zingiber officinales Rosc. Plant cell Rep. 15: 274-277.
- Smith, M.K., Hamill, S.D. 1996. Field evaluation of micropropagated and conventionally propagated ginger in subtropical Queensland. Australian J. Exp. Agricul. 36: 347-354.
- Swar, S., Pant, B. 2004. Micropropagation of Cymbidium iridiodes D. Don. In: Proceeding 4th National Conference on Science and Technology, March 23-26, RONAST, Kathmandu, Nepal.
Details
| Primary Language | English |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | July 26, 2018 |
| Submission Date | January 18, 2018 |
| Published in Issue | Year 2018 Volume: 5 Issue: 3 |
