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Türkiye'de Ticari Fasulye (Phaseolus vulgaris L.) Çeşitlerinde Bean common mosaic virus ve Bean common mosaic necrosis virus Etmenlerine Dayanıklılıkla İlişkili Genlerin Karakterizasyonu

Year 2018, Volume: 5 Issue: 4, 613 - 619, 18.10.2018
https://doi.org/10.30910/turkjans.471371

Abstract

Ülkemizde önemli bir üretim
potansiyeline sahip olan fasulyede Bean
common mosaic virus
(BCMV), Bean
common mosaic necrosis
virus
(BCMNV) viral hastalık etmenleri önemli verim kayıplarına yol açmaktadır. Son
yıllarda yaygınlaşan moleküler markörler yardımıyla dayanıklılık genlerinin
tespiti çalışmaları virüs inokulasyonu gerekmeksizin bitkilerde söz konusu
genlerin belirlenebilmesini mümkün kılmaktadır. Bu çalışmada ülkemizde yer alan
43 tescilli fasulye çeşidinde BCMV ve BCMNV virüslerine karşı muhtemel
dayanıklılık 3 farklı gen (
bc-12, I, bc-3)
ile ilişkili 4 farklı markör (SBD-5, SW-13, ROC11/420-350, eIFE4) yardımıyla incelenmiştir. Elde edilen verilere göre 43
fasulye çeşidinin BCMV ve BCMNV etmenlerine karşı dayanıklılıkta rol oynayan
bazı genlere sahip olduğu belirlenmiştir. Elde edilen markör destekli tarama
sonuçlarının virüs inokulasyonu ve patojenisite çalışmaları ile desteklenmesi
öngörülmüştür. Söz konusu markörler yardımıyla yerel fasulye çeşitlerinin ve
çeşit tescil adaylarının virüslere dayanıklılık genleri değerlendirilerek
tescil özelliklerinin güçlendirilebileceği düşünülmektedir.

References

  • Bozoğlu, H. 1995. Kuru Fasulyede Bazı Tarımsal Özelliklerin Genotip x Çevre İnteraksiyonu ve Kalıtım Derecelerinin Belirlenmesi Üzerine Bir Araştırma. Yayınlanmamış Doktora Tezi. 19 May. Ünv. Fen Bil. Enst., 99 s.
  • Collmer, C.W., Marston, M.F., Taylor, J.C., Jahn, M. 2000. The I gene of bean: a dosage-dependent allele conferring extreme resistance, hypersensitive resistance, or spreading vascular necrosis in response to the potyvirus Bean common mosaic virus. Molecular plant-microbe interactions, 13(11): 1266-1270.
  • Deligöz, İ., Sökmen, M.A. 2013. Bazı fasulye genotiplerinin Bean common mosaic virus (BCMV) ve Bean common mosaic necrosis virus (BCMNV)'a dayanıklılık durumlarının kalitatif, kantitatif ve moleküler yöntemlerle belirlenmesi. Bitki Koruma Bülteni, 53(2): 101-113
  • Deligöz, İ., Sarı, S., Karaağaç, O. 2015. Karadeniz Tarımsal Araştırma Enstitüsü tarafından geliştirilen taze fasulye ıslah hatları ve bazı ticari çeşitlerin Bean common mosaic virus (BCMV)’a dayanıklılık durumlarının araştırılması. Derim, 32(1): 1-10.
  • Drijfhout, E. 1978. Genetic Interaction Between Phaseolus vulgaris and Bean Common Mosaic Virus with Implications for Strain Identification and Breeding for Resistance. Centre for Agricultural Publication and Documents, Wageningen, the Netherlands. Feng, X., Guzmán, P., Myers, J.R., Karasev, A.V. 2017. Resistance to Bean common mosaic necrosis virus Conferred by the bc-1 Gene Affects Systemic Spread of the Virus in Common Bean. Phytopathology, 107(7): 893-900.
  • Feng, X., Myers, J.R., Karasev, A.V. 2015. Bean common mosaic virus isolate exhibits a novel pathogenicity profile in common bean, overcoming the bc-3 resistance allele coding for the mutated EIF4E translation initiation factor. Phytopathology 105: 1487-1495. Fourie, D., Miklas, P., Ariyaranthe, H. 2004. Genes conditioning halo blight resistance to races 1, 7, and 9 occur in a tight cluster. Annual Report-Bean Improvement Cooperative, 47: 103-104.
  • Haley, S.D., Afanador, L., Kelly, J.D. 1994. Identification and application of a random amplified polymorphic DNA marker for the I gene (potyvirus resistance) in common bean. Phytopathology, 84(2): 157-160.
  • Johnson, W.C., Guzmán, P., Mandala, D., Mkandawire, A.B.C., Temple, S., Gilbertson, R.L., Gepts, P. 1997. Molecular tagging of the BC-3 gene for introgression into Andean common bean. Crop Science, 37(1): 248-254.
  • Larsen, R.C., Druffel, K.L., Wyatt, S.D. 2011. The complete nucleotide sequences of bean common mosaic necrosis virus strains NL-5, NL-8 and TN-1. Arch. Virol., 156: 729-732.
  • Melotto, M., Afanador, L., Kelly, J.D. 1996. Development of a SCAR marker linked to the I gene in common bean. Genome, 39(6): 1216-1219.
  • Miklas, P.N., Stone, V., Daly, M.J., Stavely, J.R., Steadman, J.R., Bassett, M.J., Delorme, R., Beaver, J.S. 2000. Bacterial, fungal, and viral disease resistance loci mapped in a recombinant inbred common bean population ('Dorado'/XAN 176). J. Am. Soc. Hort. Sci., 125: 476-481.
  • Naderpour, M., Lund, O. S., Larsen, R., Johansen, E. 2010. Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying BC‐3 is associated with the homozygotic presence of a mutated eIF4E allele. Molecular plant pathology, 11(2): 255-263.
  • Pasev, G., Kostova, D., Sofkova, S. 2014. Identification of genes for resistance to Bean common mosaic virus and Bean common mosaic necrosis virus in snap bean (Phaseolus vulgaris L.) breeding lines using conventional and molecular methods. Journal of Phytopathology, 162(1): 19-25.
  • Strausbaugh, C.A., Miklas, P.N., Singh, S.P., Myers, J.R., Forster, R.L. 2003. Genetic characterization of differential reactions among host group 3 common bean cultivars to NL-3 K strain of Bean common mosaic necrosis virus. Phytopathology, 93(6): 683-690. TÜİK, 2017. Türkiye İstatistik Kurumu. http://www.tuik.gov.tr/ (Erişim tarihi: 25.07.2018).
  • Vetten, H.J., Lesemann, D.E., Maiss, E. 1992. Serotype A and B strains of bean common mosaic virus are two distinct potyviruses. In Potyvirus Taxonomy, pp. 415-431, Springer, Vienna.
Year 2018, Volume: 5 Issue: 4, 613 - 619, 18.10.2018
https://doi.org/10.30910/turkjans.471371

Abstract

References

  • Bozoğlu, H. 1995. Kuru Fasulyede Bazı Tarımsal Özelliklerin Genotip x Çevre İnteraksiyonu ve Kalıtım Derecelerinin Belirlenmesi Üzerine Bir Araştırma. Yayınlanmamış Doktora Tezi. 19 May. Ünv. Fen Bil. Enst., 99 s.
  • Collmer, C.W., Marston, M.F., Taylor, J.C., Jahn, M. 2000. The I gene of bean: a dosage-dependent allele conferring extreme resistance, hypersensitive resistance, or spreading vascular necrosis in response to the potyvirus Bean common mosaic virus. Molecular plant-microbe interactions, 13(11): 1266-1270.
  • Deligöz, İ., Sökmen, M.A. 2013. Bazı fasulye genotiplerinin Bean common mosaic virus (BCMV) ve Bean common mosaic necrosis virus (BCMNV)'a dayanıklılık durumlarının kalitatif, kantitatif ve moleküler yöntemlerle belirlenmesi. Bitki Koruma Bülteni, 53(2): 101-113
  • Deligöz, İ., Sarı, S., Karaağaç, O. 2015. Karadeniz Tarımsal Araştırma Enstitüsü tarafından geliştirilen taze fasulye ıslah hatları ve bazı ticari çeşitlerin Bean common mosaic virus (BCMV)’a dayanıklılık durumlarının araştırılması. Derim, 32(1): 1-10.
  • Drijfhout, E. 1978. Genetic Interaction Between Phaseolus vulgaris and Bean Common Mosaic Virus with Implications for Strain Identification and Breeding for Resistance. Centre for Agricultural Publication and Documents, Wageningen, the Netherlands. Feng, X., Guzmán, P., Myers, J.R., Karasev, A.V. 2017. Resistance to Bean common mosaic necrosis virus Conferred by the bc-1 Gene Affects Systemic Spread of the Virus in Common Bean. Phytopathology, 107(7): 893-900.
  • Feng, X., Myers, J.R., Karasev, A.V. 2015. Bean common mosaic virus isolate exhibits a novel pathogenicity profile in common bean, overcoming the bc-3 resistance allele coding for the mutated EIF4E translation initiation factor. Phytopathology 105: 1487-1495. Fourie, D., Miklas, P., Ariyaranthe, H. 2004. Genes conditioning halo blight resistance to races 1, 7, and 9 occur in a tight cluster. Annual Report-Bean Improvement Cooperative, 47: 103-104.
  • Haley, S.D., Afanador, L., Kelly, J.D. 1994. Identification and application of a random amplified polymorphic DNA marker for the I gene (potyvirus resistance) in common bean. Phytopathology, 84(2): 157-160.
  • Johnson, W.C., Guzmán, P., Mandala, D., Mkandawire, A.B.C., Temple, S., Gilbertson, R.L., Gepts, P. 1997. Molecular tagging of the BC-3 gene for introgression into Andean common bean. Crop Science, 37(1): 248-254.
  • Larsen, R.C., Druffel, K.L., Wyatt, S.D. 2011. The complete nucleotide sequences of bean common mosaic necrosis virus strains NL-5, NL-8 and TN-1. Arch. Virol., 156: 729-732.
  • Melotto, M., Afanador, L., Kelly, J.D. 1996. Development of a SCAR marker linked to the I gene in common bean. Genome, 39(6): 1216-1219.
  • Miklas, P.N., Stone, V., Daly, M.J., Stavely, J.R., Steadman, J.R., Bassett, M.J., Delorme, R., Beaver, J.S. 2000. Bacterial, fungal, and viral disease resistance loci mapped in a recombinant inbred common bean population ('Dorado'/XAN 176). J. Am. Soc. Hort. Sci., 125: 476-481.
  • Naderpour, M., Lund, O. S., Larsen, R., Johansen, E. 2010. Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying BC‐3 is associated with the homozygotic presence of a mutated eIF4E allele. Molecular plant pathology, 11(2): 255-263.
  • Pasev, G., Kostova, D., Sofkova, S. 2014. Identification of genes for resistance to Bean common mosaic virus and Bean common mosaic necrosis virus in snap bean (Phaseolus vulgaris L.) breeding lines using conventional and molecular methods. Journal of Phytopathology, 162(1): 19-25.
  • Strausbaugh, C.A., Miklas, P.N., Singh, S.P., Myers, J.R., Forster, R.L. 2003. Genetic characterization of differential reactions among host group 3 common bean cultivars to NL-3 K strain of Bean common mosaic necrosis virus. Phytopathology, 93(6): 683-690. TÜİK, 2017. Türkiye İstatistik Kurumu. http://www.tuik.gov.tr/ (Erişim tarihi: 25.07.2018).
  • Vetten, H.J., Lesemann, D.E., Maiss, E. 1992. Serotype A and B strains of bean common mosaic virus are two distinct potyviruses. In Potyvirus Taxonomy, pp. 415-431, Springer, Vienna.
There are 15 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Mehmet Zahit Yeken This is me

Göksel Özer This is me

Ali Çelik This is me

Vahdettin Çiftçi This is me

Publication Date October 18, 2018
Submission Date September 7, 2018
Published in Issue Year 2018 Volume: 5 Issue: 4

Cite

APA Yeken, M. Z., Özer, G., Çelik, A., Çiftçi, V. (2018). Türkiye’de Ticari Fasulye (Phaseolus vulgaris L.) Çeşitlerinde Bean common mosaic virus ve Bean common mosaic necrosis virus Etmenlerine Dayanıklılıkla İlişkili Genlerin Karakterizasyonu. Türk Tarım Ve Doğa Bilimleri Dergisi, 5(4), 613-619. https://doi.org/10.30910/turkjans.471371